Analysis of Protein Separation Mechanism in Charged Ultrafiltration Membrane

Danu Ariono, Putu Teta P. Aryanti, Anita Kusuma Wardani, I Gede Wenten

Abstract


The separation mechanism of proteins on a charged ultrafiltration membrane was analyzed using the extended Nernst–Planck (N-P) model. The model was solved numerically based on experimental data during ultrafiltration of bovine serum albumin/BSA and hemoglobin at various pH (between 5 and 8) to obtain the flux parameter (Jv). The flux parameter was used to determine the effective charge of the membrane (f) and the actual membrane porosity (Ak). These two parameters were then used to predict the transport mechanism of proteins through the charged membrane. Higher flux was obtained during the ultrafiltration of BSA compared to hemoglobin. The most effective separation of mixed proteins occurred at pH 5 (aalbumin= 5). In addition, the mobility of a single protein was lower than when it was mixed with other proteins that had different charges. The effective charges of the membranes were varied between 0.99996 to 1.0000, which means that the fixed charge on the membrane structure was higher than the concentration of proteins, thus the effective charge of the membrane was not influenced by the presence of protein charge at various pH. On the contrary, the value of Ak was influenced by the type and charge of the proteins. A decrease of negative charge along with an increase of solution pH increased the porosity of the membrane, thus reducing the rejection of proteins.

Keywords


BSA; charged membrane; Nernst–Planck model; protein separation; ultrafiltration

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References


Drioli, E., Stankiewicz, A.I. & Macedonio, F., Membrane Engineering in Process Intensification-An Overview, Journal of Membrane Science, 380(1), pp. 1-8. 2011

Bernardo, P. & Drioli, E., Membrane Gas Separation Progresses for process Intensification Strategy in the Petrochemical Industry, Petroleum Chemistry, 50(4), pp. 271-282, 2010.

Baker, R.W., Future Directions of Membrane Gas Separation Technology, Industrial & Engineering Chemistry Research, 41(6), pp. 1393-1411, 2002.

Khoiruddin, Aryanti, P.T.P, Hakim, A.N. & Wenten, I.G., The Role of Ion-exchange Membrane in Energy Conversion, in AIP Conference Proceedings, AIP Publishing, 1840(1), 090008, 2017.

Ariono, D., Aryanti, P.T.P., Subagjo, S. & Wenten, I.G., The Effect of Polymer Concentration on Flux Stability of Polysulfone Membrane, in AIP Conference Proceedings. AIP Publishing, 1788, 030048, 2017.

Wardani, A.K., Hakim, A.N., Khoiruddin & Wenten, I.G., Combined Ultrafiltration-Electrodeionization Technique for Production of High Purity Water, Water Science and Technology, pp. 2017173, 2017.

Wenten, I.G., Dharmawijaya, P.T., Aryanti, P.T.P., Mukti, R.R. & Khoiruddin, LTA Zeolite Membranes: Current Progress and Challenges in Pervaporation, RSC Advances, 7(47), pp. 29520-29539, 2017.

Sianipar, M., Kim, S.H., Khoiruddin, Iskandar, F. & Wenten, I.G., Functionalized Carbon Nanotube (CNT) Membrane: Progress and Challenges. RSC Advances. 7(81), pp. 51175-51198, 2017.

Mangindaan, D., Khoiruddin, K. & Wenten, I.G., Beverage Dealcoholization Processes: Past, Present, and Future, Trends in Food Science & Technology, 71, 36-45, 2017.

Strathmann, H., Electrodialysis, a Mature Technology with a Multitude of New Applications, Desalination, 264(3), pp. 268-288, 2010.

Aryanti, P.T.P., Sianipar, M., Zunita, M. & Wenten, I.G., Modified Membrane with Antibacterial Properties, Membrane Water Treatment, 8(5), pp. 463-481, 2017.

Himma, N.F., Prasetya, N., Anisah, S. & Wenten, I.G., Superhydrophobic Membrane: Progress in Preparation and its Separation Properties. Reviews in Chemical Engineering, DOI: 10.1515./revce-2017-0030, 2018.

Purwasasmita, M., Kurnia, D., Mandias, F. Khoiruddin & Wenten, I.G., Beer Dealcoholization using Non-porous Membrane Distillation. Food and Bioproducts Processing, 94, pp. 180-186. 2015.

Saxena, A., Tripathi, B.P., Kumar, M. & Shahi, V.K., Membrane-based Techniques for the Separation and Purification of Proteins: an Overview, Advances in Colloid and Interface Science, 145(1), pp. 1-22, 2009.

Striemer, C.C., Gaborski, T.R., McGrath, J.L. & Fauchet, P.M., Charge-and Size-based Separation of Macromolecules using Ultrathin Silicon Membranes, Nature, 445(7129), pp. 749, 2007.

Xu, Y. & Lebrun, R.E., Investigation of the Solute Separation by Charged Nanofiltration Membrane: Effect of pH, Ionic Strength and Solute Type. Journal of Membrane Science, 158(1), pp. 93-104, 1999.

Ma, J., Qin, L., Zhang, X. & Huang, H., Temporal Evolution of the Selectivity-permeability Relationship during Porous Membrane Filtration of Protein Solutions, Journal of Membrane Science, 514, pp. 385-397, 2016.

Jones, K.L. & O’Melia, C.R., Protein and Humic Acid Adsorption onto Hydrophilic Membrane Surfaces: Effects of pH and Ionic Strength, Journal of Membrane Science, 165(1), pp. 31-46, 2000.

Smith, S.C., Ahmed, F., Gutierrez, K.M. & Rodrigues, D.F., A Comparative Study of Lysozyme Adsorption with Graphene, Graphene Oxide, and Single-walled Carbon Nanotubes: Potential Environmental Applications, Chemical Engineering Journal, 240, pp. 147-154, 2014.

Tercinier, L., Ye, A., Singh, A., Anema, S.G. & Singh, H., Effects of Ionic Strength, pH and Milk Serum Composition on Adsorption of Milk Proteins on to Hydroxyapatite Particles, Food Biophysics, 9(4), pp. 341-348, 2014.

Li, L., Shi, X., Guo, X., Li, H. & Xu, C., Ionic Protein–lipid Interaction at the Plasma Membrane: What Can the Charge Do?, Trends in Biochemical Sciences, 39(3), pp. 130-140, 2014.

Low, S.C., Shaimi, R., Thandaithabany, Y., Lim, J.K., Ahmad, A.L. & Ismail, A., Electrophoretic Interactions between Nitrocellulose Membranes and Proteins: Biointerface Analysis and Protein Adhesion Properties, Colloids and Surfaces B: Biointerfaces, 110, pp. 248-253, 2013

Chakrabarty, T. & Shahi, V.K., Modified Chitosan-based, pH-responsive Membrane for Protein Separation, RSC Advances, 4(95), pp. 53245-53252, 2014.

Mulder, J., Basic Principles of Membrane Technology, Springer Science & Business Media, 1996.

Galama, A., Post, J., Stuart, M.C. & Biesheuvel, P., Validity of the Boltzmann Equation to Describe Donnan Equilibrium at the Membrane–solution Interface, Journal of Membrane Science, 442, pp. 131-139, 2013.

Moučka, F., Nezbeda, I. & Smith, W.R., Chemical Potentials, Activity Coefficients, and Solubility in Aqueous NaCl Solutions: Prediction by Polarizable Force Fields, Journal of Chemical Theory and Computation, 11(4), pp. 1756-1764, 2015.

Wei, Z. & Semiat, R., Applying a Modified Donnan Model to Describe the Surface Complexation of Chromate to Iron Oxyhydroxide Agglomerates with Heteromorphous Pores, Journal of Colloid and Interface Science, 506, pp. 66-75, 2017.

Schultz, S.G., Basic Principles of Membrane Transport, 2, CUP Archive, 1980.

Samson, E. & Marchand, J., Numerical Solution of the Extended Nernst–Planck Model, Journal of Colloid and Interface Science, 215(1), pp. 1-8, 1999.

Tsuru, T., Nakao, S-I. & Kimura, S., Calculation of Ion Rejection by Extended Nernst–Planck Equation with Charged Reverse Osmosis Membranes for Single and Mixed Electrolyte Solutions, Journal of Chemical Engineering of Japan, 24(4), pp. 511-517, 1991.

Afonso, M.D. & de Pinho, M.N., Transport of MgSO4, MgCl2, and Na2SO4 Across an Amphoteric Nanofiltration Membrane, Journal of Membrane Science, 179(1), pp. 137-154, 2000.

Bowen, W.R. & Mukhtar, H., Characterisation and Prediction of Separation Performance of Nanofiltration Membranes, Journal of Membrane Science, 112(2), pp. 263-274, 1996.

Dresner, L., Some Remarks on the Integration of the Extended Nernst–Planck Equations in the Hyperfiltration of Multicomponent Solutions, Desalination, 10(1), pp. 27-46, 1972.

Schlögl, R., Membrane Permeation in Systems Far from Equilibrium, Berichte der Bunsengesellschaft für physikalische Chemie, 70(4), pp. 400-414, 1966.

Kobatake, Y. & Kamo, N., Transport Processes in Charged Membranes, Prog. Polymer Sci. Japan, 5, pp. 257-302, 1973.

Bailey, M.P., Rocks, B.F. & Riley, C., Homogeneous Fluoroimmunoassay Using Lucifer Yellow VS: Determination of Albumin Plasma, Annals of Clinical Biochemistry, 21(1), pp. 59-63, 1984.

Warawa, J., Finlay, B.B. & Kenny, B., Type III secretion-dependent hemolytic activity of enteropathogenic Escherichia coli, Infection and Immunity, 67(10), pp. 5538-5540, 1999.

Abramson, H.A., Moyer, L.S. & Gorin, M.H., Electrophoresis of Proteins and the Chemistry of Cell Surfaces, Electrophoresis of Proteins and the Chemistry of Cell Surfaces, 1942.

Wang, K.Y. & Chung, T-S., The Characterization of Flat Composite Nanofiltration Membranes and their Applications in the Separation of Cephalexin, Journal of Membrane Science, 247(1), pp. 37-50, 2005.

Hilal, N., Al-Abri, M., Al-Hinai, H. & Abu-Arabi, M., Characterization and Retention of NF Membranes using PEG, HS and Polyelectrolytes. Desalination, 221(1), pp. 284-293, 2008.

Zdunek, A.D. & Selman, J.R., Mass Transfer and Complexation in Concentrated Zinc/potassium Halide Electrolytes-II, Complexation And Migration Effects, Electrochimica Acta, 34(10), pp. 1461-1471, 1989.




DOI: http://dx.doi.org/10.5614%2Fj.eng.technol.sci.2018.50.2.4

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