Antihyperglycemic Activity of Piper crocatum Leaves and Cinnamomum burmannii Bark Mixture Extract in Streptozotocin-induced Diabetic Rats
Keywords:antihyperglycemic agents, Cinnamomum burmannii, glucose, insulin, lipid profile, Piper crocatum.
AbstractIndonesia presently has the fourth largest diabetic mellitus prevalence of all countries in the world. In a previous study, a mixture extract of Piper crocatum leaves and Cinnamomum burmannii bark showed in vitro antihyperglycemic activity. It acted as inhibitor of the a-glucosidase enzyme and had no toxic effect when it was administered orally to male and female rats for 28 days. In the present study, mixture extracts of P. crocatum leaves and C. burmannii bark were used to observe antihyperglycemic activity in diabetic rats induced with streptozotocin. Mixture extracts of P. crocatum leaves and C. burmannii bark were orally given to diabetic Sprague Dawley rats at various doses for 16 days. The results showed that the treatment led to a reduction of the blood glucose level, an increase in blood insulin level up to 170.75% at 1260 mg/kg body weight, maintaining the blood lipid level of the diabetic rats at a normal level, and an increase of pancreatic ÃŸ cells in the islets of Langerhans up to 2.2-fold at 1260 mg/kg body weight. The mixture extracts of P. crocatum and C. burmannii have antihyperglycemic activity, which enhances the number of pancreatic Î² cells.
American Diabetes Association, Standards of Medical Care in Diabetes-2011, Diabetes Care, 35(1), pp. 564-571, 2012.
King, H., Aubert, R.E. & Herman, W.H., Global Burden of Diabetes 1995-2025: Prevalence, Numerical Estimates and Projections, Diabetes Care, 21(9), pp. 1414-1431, 1998.
Boyle, J.P., Honeycutt, A.A., Narayan, K.M., Hoergoer, T.J., Geiss, L.S., Chen, H. & Thompson, T.J., Projection of Diabetes Burden through 2050: Impact of Changing Demography and Disease Prevalence in the U.S., Diabetes Care, 24(11), pp. 1936-1940, 2001.
Rates, S.M., Plants as A Source of Drugs, Toxicon, 39(5), pp. 603-613, 2001.
Safithri, M. & Fahma, F., Potency of Piper crocatum Decoction as an Antihiperglycemia in Rat Strain Sprague Dawley, Hayati Journal of Bioscience, 15(1), pp. 45-48, 2008.
Satyanarayana, T., Katyayani, B.M., Hema, L.E., Anjana, A.M. & Chinna, E. M., Hypoglycemic and Anti-hyperglycemic Effect of Alcoholic Extract of Euphorbia Leucophylla in Normal and in Alloxan Induced Diabetic Rats, Pharmacoognosy Magazine, 2(8), pp. 244-255, 2006.
Battu, G.R., Mamidipalli, S.N., Parimi, R., Viriyala, R.K., Patchula, R.P. & Mood, L.R., Hypoglycemic and Anti-Hyperglycemic Effect of Alcoholic Extract of Benincasa Hispida in Normal and in Alloxan Induced Diabetic Rats, Pharmacoognosy Magazine, 3(10), pp. 101-105, 2007.
Tapas, A.R., Sakarkar, D.M. & Kakde, R.B., Flavonoids as Nutraceuticals: A Review, Tropical Journal of Pharmaceutical Research, 7(3), pp. 1089-1099, 2008.
Shan, B., Cai, Y.Z., Brooks, J.D. & Corke, H., The In-vitro Antibacterial Activity of Dietary Spice and Medicinal Herb Extracts, International Journal of Food Microbiology, 117(1), pp. 112-119, 2007.
Preuss, H.G., Echard, B., Polansky, M.M. & Anderson, R., Whole Cinnamon and Aqueous Extracts Ameliorate Sucrose-induced Blood Pressure Elevations in Spontaneously Hypertensive Rats, Journal of the American College of Nutrition, 25(2), pp. 144-150, 2006.
Khan, A., Safdar, M., Khan, M.M.A., Khattak, K.N. & Anderson, R.A., Cinnamon Improves Glucose and Lipids of People with Type 2 Diabetes, Diabetes Care, 26(12), pp. 3215-3218, 2003.
Falah, S., Safithri, M., Katayama, T. & Suzuki, T., Hypoglycemic Effect of Mahagony (Swietenia macrophylla king) Bark Extracts in Alloxan-induced Diabetic Rats, Wood Research Journal, 2(1), pp. 89-94, 2010.
Mallick, C., Chatterjee, K., Guha Biswas, M. & Ghosh, D., Antihyperglycemic Effects of Separate and Composite Extract of Root of Musa Paradisiaca and Leaf of Coccinia Indica in Streptozotocin-induced Diabetic Male Albino Rat, Afr. J. Trad. CAM, 4, pp. 362-371.
Odutuga, A.A., Dairo, J.O., Minari, J.B. & Bamisaye, F.A., Antidiabetic Effect of Morinda lucida Steam Bark Extracts on Alloxan-induced Diabetic Rats, Research Journal of Pharmacology, 4(3), pp. 78-82, 2010.
Paranagama, P.A., Wimalasena, S., Jayatilake, G.S., Jayawardena, A.L., Senanayake, U.M. & Mubarak, A.M., A Comparison of Essential Oil Constituents of Bark, Leaf, Root and Fruit of Cinnamon (Cinnamomum zeylanicum Blum) Grown in Sri Lanka, J. Natn. Sci. Foundation Sri Lanka, 29, pp. 147-153, 2001.
Schmidt, E., Jirovetz, L., Buchbauer, G., Eller, G.A., Stoilova, I., Krastanov, A.I., Stoyanova, A. & Geissler, M., Composition and Antioxidant Activities of the Essential Oil of Cinnamon (Cinnamomun zeylanicum Blume) leaves from Sri Lanka, Journal of Essential Oil Bearing Plants, 9(2), pp. 170-182, 2006.
Jadhav, R. & Puchchakayala G., Hypoglycemic and Antidiabetic Activity of Flavonoids: Boswellic Acid, Ellagic Acid, Quercetin, Rutin on Streptozotocin-Nicotinamide Induced Type 2 Diabetic Rats, International Journal of Pharmacy and Pharmaceutical Sciences, 4(2), 2012.
Wisetmuen, E., Pannangpetch, P., Kongyingyoes, B., Kukongviriyapan., U., Yutanawiboonchai, W. & Itharat, A., Insulin Secretion Enhancing Activity of Roselle Calyx Extract in Normal and Streptozotocin-induced Diabetic Rats, Pharmacognosy Research, 5(2), pp. 65-71, 2013.
Singh, N., & Gupta, M., Regeneration of Cells in Islets of Langerhans of Pancreas of Alloxan Diabetic Rats by Acetone Extract of Momordica Charantia (Linn.) (Bitter Gourd) Fruits, Indian Journal of Experimental Biology, 45, pp. 1055-1062, 2007.
Szkdelski, T., The Mechanism of Alloxan and Streptozotocin Action in Cells of the Rat Pancreas, Physiological Research., 50(1), pp. 536-546, 2001.
Karaca, T., Yoruk, M., Yoruk, I.H. & Uslu, S., Effect of Extract Green Tea and Gingseng on Pancreatic Beta Cells and Levels of Serum Glucose, Insulin, Cholesterol, Triglycerides In Rats with Experimentally Streptozotocin Induced Diabetes: A Histochemical And Immunohistochemical Study, Journal of Animal Veterinary Advances, 9(1), pp. 102-107, 2010.