Flavonoid Compounds from the Herb of Krokot (Lygodium microphyllum) and their antioxidant activity against DPPH

Hadi Kuncoro, Kindi Farabi, Laode Rijai, Euis Julaeha, Unang Supratman, Yoshihito Shiono


Six flavonoid compounds, kaempferol (1), quercetin (2), acacetin (3), quercetin-3-O-β-D-glucopyranoside (4), kaempferol-3-O-β-D-glucopyranoside (5), and isorhamnetin-3-O-β-D-glucopyranoside (6), were isolated from the methanol extract of the Lygodium microphyllum herb. The chemical structures of compounds 1-6 were identified on the basis of spectroscopic evidence and comparison with previously reported spectral data. Compounds 1-6 were isolated for first time from this plant. Their antioxidant activity against DPPH (2,2-Diphenyl-1-picrylhydrazyl) was evaluated. Among the compounds, quercetin (2) showed high antioxidant activity with IC50 values of 6.94 ± 0.03 μg/mL.


Antioxidant; DPPH; flavonoids; Lygodium microphyllum; Lygodiaceae

Full Text:



Zeng-Fu, L., Huil, H., Hang-Yi, Z. & Jun-Chen, Z., Review on the Extraction of Flavonoids from Fern, Journal of Sanming University, 25(2), pp. 200-203, 2008.

Guo-Gang, Z., Ying-Cui, H., Hong-Xia, L., Lin-Xia, Z. & Li-Juan, C., The Research of Lygodium, Intechopen, Shanghai, 2012.

Zheng, X.L. & Xing, F.W., Ethnobotanical Study on Medicinal Plants around Mt. Yinggeling, Hainan Island, China, Journal of Ethnopharmacology, 124(2), pp. 197-210, 2009.

Zhang, L., Yin, Z. & Ye, W., Flavonoids from Lygodium Japonicum, Biochemical Systematics and Ecology, 34, pp. 885-886, 2006.

Ye, W., Fan, C., Zhang, L., Yin, Z. & Zhao, S., A New Phenolic Glycoside from the Roots of Lygodium Japonicum, Fitoterapia, 78(7-8), pp. 600-601, 2007.

Chen, L., Zhang, G., He, J., Jin Guan, J., Pan, C., Mi, W. & Wang, Q., New Naphthoquinone from the Root of Lygodium Japonicum (Thunb.) Sw. Journal of Natural Medicine, 64, pp. 114-116, 2010.

Zhu, L., Zhang, G., Chen, L., Wang, S., Li, P. & Li, L., A New Ecdysteroside from Lygodium japonicum (Thunb.) Sw, Journal of Natural Medicinal, 63, pp. 215-219, 2009.

Katja, D.G., Sonda, A.A., Harneti, D.P., Mayanti, T. & Supratman, U., 7-hydroxy-6-metoxy cumarin (schopoletin) from the Bark of Chisocheton celebicus (Meliaceae), Jurnal Kimia Indonesia, 1, pp. 10-14, 2017. (Text in Indonesian)

Duan, Y., Dai, He, Y.R., Kurihara, H., Li, Y. & Yao, X., A New Phenylpropanoid Glucoside from the Aerial Parts of Lygodium Japonicum, Journal of Asian Natural Products Research, 14(3), pp. 286-292, 2012.

Lei, J., Xiao, Y., Huang, J., Tang, M. & Deng, P., Two New Kaempferol Glycosides from Androsace Umbellata, Helvetica Chimica Acta, 92(7), pp. 1439-1444, 2009.

Chaturvedula, V.S.R. & Prakash, I., Isolation of Stigmasterol and β-sitosterol from the Dichloromethane Extract of Rubus Suavissimus, International Current Pharmaceutical Journal, 1(9), pp. 239-242, 2012.

Mouffok, S., Haba, H., Lavaud, C., Long, C. & Benkhaled, M., Chemical Constituents of Centaurea omphalotricha Coss. & Durieu ex Batt. & Trab, Records of Natural Products, 6(3), pp. 292-295, 2012.

Gomes, R.A., Ramirez, R.R.A., Maciel, J.K.S., Agra, M.F., Souza, M.F.V. & Falcao-silva, V.S., Phenolic Compounds from Sidastrum micranthum (A. St.-Hil.) fryxell and Evaluation of Acacetin and 7,4'-Di-O-methylisoscutellarein as Modulator of Bacterial Drug Resistence, Quimica Nova, 34(8), pp. 283-286, 2011.

Islam, M., Al-Amin, M.D., Siddiqi, M.M.A., Akter, S., Haque, M.M., Sultana, N. & Chowdhury, A.S., Isolation of Quercetin-3-O-beta-D-Glucopyranoside from the Leaves of Azadirachta Indica and Antimicrobial and Cytotoxic Screening of the Crude Extracts, Dhaka University Journal of Science, 60(1), pp. 11-14, 2012.

Sianturi, J., Farabi, K., Mayanti, T., Harneti, D., Darwati, Supratman, U., Awang, K. & Hayashi, H., Antioxidant Constituents from the Bark of Aglaia eximia (Meliaceae), Makara Journal of Science, 20(1), pp. 1-6, 2016.

Liu, H., Mou, Y., Zhao, J., Wang, J., Zhou, L., Wang, M., Wang, D., Han, J., Yu, Z. & Yang, F., Flavonoids from Halostachys caspica and their Antimicrobial Antioxidant Activities, Molecules, 15(11), pp. 7933-7945, 2010.

DOI: http://dx.doi.org/10.5614%2Fj.math.fund.sci.2018.50.2.7


  • There are currently no refbacks.

View my Stats

Creative Commons License
This work is licensed under a Creative Commons Attribution-NoDerivatives 4.0 International License.


ITB Journal Publisher, LPPM ITB, Center for Research and Community Services (CRCS) Building, 6th & 7th Floor, Jalan Ganesha 10, Bandung 40132, Indonesia, Phone: +62-22-86010080, Fax.: +62-22-86010051; E-mail: jmfs@lppm.itb.ac.id