Optimization of Extraction Method and Characterization of Phycocyanin Pigment from Spirulina platensis

Elin Julianti, Susanti Susanti, Marlia Singgih, Laida Neti Mulyani

Abstract


Spirulina platensis is a microalgae species that contains phycocyanin, a blue pigment that is widely used in the food, cosmetic and pharmaceutical industries. The objective of this study was to isolate phycocyanin from dried biomass of Spirulina platensis. The optimum extraction was through freeze-thawing extraction with 0.01 M phosphate buffer pH 7. Purification was done using 50% ammonium sulphate precipitation, dialysis, and gel filtration chromatography. The purity ratio of the purified product was 1.729 (A615/A280). Characterization of isolate, carried out using SDS-PAGE, gave molecular weights of around 15-16 Kda and using infrared spectrophotometry the similarity with the standard phycocyanin spectrum was obtained. Both methods confirmed that the isolate was phycocyanin.


Keywords


freeze-thawing; microalgae; phycocyanin; purity ratio; SDS-PAGE; Spirulina platensis

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References


Sarada, R., Manoj, G.P & Ravinshkar, G.A., Phycocyanin from Spirulina sp: Influence of Processing of Biomass on Phycocyanin Yield, Analysis of Efficacy of Extraction Methods and Stability Studies on Phycocyanin, Process Biochem, 34(8), pp. 795-801, 1999. DOI:10.1016/S0032-9592(98)00153-8

Moraes, C.C., Sala, L., Cerveira, G.P. & Kalil, S.J., C-Phycocyanin Extraction from Spirulina platensis Wet Biomass, Braz. J. Chem. Eng. 28(1), pp. 45-49, 2011. DOI:10.1590/S0104-66322011000100006

Bennett, A. & Bogorad, L., Complementary Chromatic Adaption in A Filamentous Blue-green Alga, J. Cell. Biol., 58(2), pp. 419-435, 1973. DOI:10.1083/jcb.58.2.419

Pirenantyo, P. & Limantara L., Pigmen Spirulina as Anti-cancer Substance, Indonesian Journal of Cancer, 2(4), pp. 155-163, 2008. DOI: 10.33371/ijoc.v2i4.61

Ferreira, H.A., Torres-Duran, P.V. & Juarez-Oropeza, M.A., Hepatoprotective Effects of Spirulina maxima in Patients with Nonalcoholic Fatty Liver Disease: A Case Series, J. Med. Case. Reports., 4(103), 2010. DOI:10.1186/1752-1947-4-103

Gantar, M., Simovic, D., Djils, S., Gonzalez, W.W. & Miksovska J., Isolation, Characterization and Antioxidative Activity of C-phycocyanin from Limnothrix sp. Strain 37-2-1, J. Biotechnol., 159(1-2), pp. 21-26, 2012. DOI:10.1016/j.jbiotec.2012.02.004

Patel, A., Mishra, S., Pawar, R. & Ghosh, P.K., Purification and Characterization of C-Phycocyanin from Cyanobacterial Species of Marine and Freshwater Habitat, Protein. Expr. Purif., 40(2), pp. 248-255, 2005. DOI:10.1016/j.pep.2004.10.028

Liu, Y., Xu, L., Cheng, N., Lin, L. & Zhang, C., Inhibitory Effect of Phycocyanin from Spirulina platensis on the Growth of Human Leukemia K562 Cells, J. Appl. Phycol., 12(2), pp. 125-130, 2000. DOI:10.1023/A: 1008132210772

Sekar, S. & Chandramohan, M., Phycobiliproteins as a Commodity: Trends in Applied Research, Patents, and Commercialization, J. Appl. Phycol., 20(2), pp. 113-136, 2008. DOI:10.1007/s10811-007-9188-1

Su, C., Liu, C., Yang, P., Syu, K. & Chiuh, C., Solid-liquid Extraction of Phycocyanin from Spirulina platensis: Kinetic Modeling of Influential Factors, Sep. Purif. Technol., 123, pp. 64-68, 2014. DOI: 10.1016/ j.seppur.2013.12.026

Abalde, J., Betancourt, L., Torres, E., Cid, A. & Barwell C., Purification and Characterization of Phycocyanin from The Marine Cyanobacterium Synechococcus sp. IO9201, Plant Sci., 136(1), pp. 109-120, 1998. DOI:10.1016/S0168-9452(98)00113-7

Reis, A., Mendes, A., Lobo-Fernandes, H., Empis, J.A. & Novais, J.M., Production, Extraction, and Purification of Phycobiliproteins from Nostoc Sp., Bioresour. Technol., 66(3), pp. 181-187, 1998. DOI:10.1016/ S0960-8524(98)00064-9

Majdoub, H., Ben, M.M., Chaubet, F., Roudesli, M.S. & Maaroufi, R.M., Anticoagulant Activity of a Sulfated Oligosaccharide from The Green Alga Arthrospira Plantensis, Biochim. Biophys. Acta., 1790(10), pp. 1377-1381, 2009. DOI: 10.1016/j.bbagen.2009.07.013

Rito-Palomares, M., Nuñez, L. & Amador, D., Practical Application of Aqueous Two-Phase Systems for The Development of a Prototype Process for C-Phycocyanin Recovery from Spirulina maxima, J. Chem. Technol. Biotechnol., 76(12), pp. 1273-1280, 2001. DOI:10.1002/jctb. 507

Kumar, D., Dhar, D.W., Pabbi, S., Kumar, N. & Walia, S., Extraction, and Purification of C-Phycocyanin from Spirulina Platensis (CCC540), Ind. J. Plant. Physiol., 19(2), pp. 184-188, 2014. DOI: 10.1007/s40502-014-0094-7

Hegelund, F., Larsen, R.W. & Palmer, M.H, The High Resolution Infrared Spectrum of Pyrrole Between 900 and 1500 cm-1 Revisited, J. Mol. Spectrosc., 252(1), pp. 93-97, 2008. DOI: 10.1016/j.jms.2008.06. 010




DOI: http://dx.doi.org/10.5614%2Fj.math.fund.sci.2019.51.2.6

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