Robusta Coffee (Coffeacanephora) Decreasing IL-1α (Interleukin-1α) Expression and Increasing the Number of Fibroblasts in Healing Process in Dental Pulp in Wistar Rats

I Dewa Ayu Ratna Dewanti, I Dewa Ayu Susilawati, Pujiana Endah Lestari, Ristya Widi Endah Lestari, Erawati Wulandari, Roedy Budirahardjo, Dyah Setyorini, Sunlip Wibisono

Abstract


Fibroblasts and IL-1α are important components of inflammation and healing in dental caries. This healing allegedly could be enhanced by robusta coffee. The aim of this study was to investigate the effect of varying concentrations of robusta coffee on IL-1α expression and the number of fibroblasts. Wistar rats were used, divided into 4 groups and 3 subgroups (7-, 14‑, and 21-day rats) with each group containing 4 animals: Control group: untreated group; 25% Coffee Group: cavities + treated with 25% coffee bean paste; 50% Coffee Group: cavities + treated with 50% coffee bean paste; 75% Coffee Group: cavities + treated with 75% coffee bean paste. The 7-, 14-, and 21-day rats were sacrificed serially for analysis of the number of dental pulp fibroblasts by staining with HE and IL-1α expression by immunohistochemistry. The resulting data were analyzed descriptively and using ANOVA, followed by an LSD test. The higher the concentration of robusta coffee, the more the expression of IL-1α decreased and the more the number of fibroblasts increased. It is suspected that immunomodulatory proteins inhibit IL-1α expression. It was concluded that robusta coffee could decrease IL-1α expression and increase the number of fibroblasts during the healing process in the dental pulp of Wistar rats.

Keywords


dental pulp; fibroblasts; IL-1α; robusta coffee; Wistar rat

Full Text:

PDF

References


Lockhart, P.B., Brennan, M.T., Thornhill, M., Michalowicz, B.S., Noll, J., Bahrani-Mougeot, F.K. & Sasser, H.C., Poor Oral Hygiene as A Risk Factor for Infective Endocarditis-Related Bacteremia, J. Am. Dent. Assoc., 140(10), pp. 1238-1244, 2009.

Nomura, R., Nakano Knemoto H., Fujita, K., Inagaki S., Takahashi, T., Taniguchi, K., Takeda, M. & Yoshioka, Isolation and Characterization of Streptococcus mutans in Heart Valve and Dental Plaque Specimens From A Patient with Infective Endocarditis, J. Med. Microbiol, 55(8), pp. 1135-1140, 2006.

Health Research and Development Agency, Basic Health Research 2007 (Riset Kesehatan Dasar/RISKESDAS 2007), Ministry of Health, Republic of Indonesia, December, 2008. (Text in Indonesian)

Rahardjo, A., Maharani, D.A., A Review of Indonesia’s Dental Health - Past, Present and Future, Int. J. Clin. Prev. Dent., 10(3), pp. 121-126, 2014.

Ng, N., Kaye, E.K. & Garcia R.I., Coffee Consumption and Periodontal Disease in Males, Journal of Periodontology, 85(8), pp. 1042-1049, 2014. DOI: 10.1902/jop.2013.130179

Scalbert, A. & Williamson G., Dietary Intake and Bioavailability of Polyphenols, J. Nutr., 130(8), pp. 2073S-2085S, 2000.

Dupas, C.J., Marsset-Baglieri, A.C., Ordonaud, C.S., Ducept, F.M.G. & Maillard, M.N., Coffee Antioxidant Properties: Effects of Milk Addition and Processing Conditions, Issue Journal of Food Science, 71(3), pp. S253-S258, 2006.

Natella, F., Nardini, M., Belelli, F., Pignatelli,P., Di Santo,S., Ghiselli,A., Violi,F. & Scaccini C., Effect of Coffee Drinking on Platelets: Inhibition of Aggregation and Phenols Incorporation, Br J Nutr. 2008 Dec., 100(6): pp. 1276-82, 2008. DOI: 10.1017/S0007114508981459

Kallmyer, T. & Foster, J., 19 Good Health Reasons to Drink Coffee, Caffeine Informer, https://www.caffeineinformer.com/7-good-reasons-to-drink-coffee/, (23 January 2017)

Vu, L., Coffee can Decrease Tooth Decay, Web Dental, http://www. webdental.com/profiles/blogs/coffee-can-decrease-tooth-decay, (23 Janu-ary 2017).

Namboodiripad, P. & Srividya, K., Can Coffee Prevent Caries? An In-Vitro Study, The Internet Journal of Dental Science, 7(2), 2009. DOI: 10.5580/101c

Namboodiripad, P.C.A. & Kori, S., Can Coffee Prevent Caries? J.Conserv. Dent., 12(1), pp. 17-21, 2009.

Dewanti Ratna, I.D.A., Potential of Coffee Seeds as Immunomodulator to Prevent and Overcome Dental Caries, 9th International Dental Federation - Indonesia Dental Association Joint Meeting Yogyakarta, Indonesia, November 16th – 17th, 2013

Endah, L.P., Dewanti Ratna, I.D.A. & Budirahardjo, R., Robusta Coffee Beans Increase Levels of TNF-Α as A Response to Streptoccus Mutans, 1st International Conference on Medicine and Health Sciences, pp. 162-168, Aug 31 st – Sep 1st, 2016.

Dewanti, Ratna I.D.A, Robusta Coffee Beans Decrease of Inflammation in Dental Caries, 1st International Conference on Medicine and Health Sciences, pp. 173-176, Aug 31st – Sep 1st, 2016.

Di Palo, N.C. & Shayakhmetov, D.M, Interleukin 1a and the Inflammatory Process, Nature Immunology, 17, pp. 906-913, 2016.

Rider, P., Carmi, Y., Guttman, O., Braiman, A., Cohen, I., Voronov, E., White, M.R., Dinarello, C.A., Apte, R.N., IL-1α and IL-1β Recruit Different Myeloid Cells and Promote Different Stages of Sterile Inflammation, J. Immunol., 187(9), pp. 4835-4843, 2011. DOI: 10.4049/ jimmunol.1102048

Suwara, M.I., Green, N.J., Borthwick, L.A., Mann, J., Mayer-Barber, K.D., Barron, L., Corris, P.A., Farrow, S.N., Wynn T.A., Fisher A.J. & Mann, D.A., IL-1α Released from Damaged Epithelial Cells is Sufficient and Essential to Trigger Inflammatory Responses in Human Lung Fibroblasts Open, Mucosal Immunology, 7, pp. 684-693, 2014. DOI:10.1038/mi.2013.87

Nishi, O.,Nishi, K. & Ohmoto, Y., Synthesis of Interleukin-1, Interleukin-6, and Basic Fibroblast Growth Factor by Human Cataract Lens Epithelial Cells, Journal of Cataract & Refractive Surgery, 22(Suppl. 1), pp. 852-858, 2017. DOI:10.1016/S0886-3350(96)80174-X

Luger, T.A. & Schwarz, T., The Role of Cytokines and Neuroendocrine Hormones in Cutaneous Immunity and Inflammation. Allergy, 50(4), pp. 292-302, 1995.

Sri, M. & Suharyanto, E., Coffee, Steeping, and Health, Coffee and Cocoa Research Center Indonesia, Jember, 2015.

Ardo, S., Response of Rat Dental Pulp to Propolis as Direct Pulp Material, Indonesia Dental Journal, Special Edition KPPIKG XIV, pp. 57-61, 2006. (Text in Indonesian)




DOI: http://dx.doi.org/10.5614%2Fj.math.fund.sci.2019.51.1.6

Refbacks

  • There are currently no refbacks.


View my Stats

Creative Commons License
This work is licensed under a Creative Commons Attribution-NoDerivatives 4.0 International License.

 

ITB Journal Publisher, LPPM ITB, Center for Research and Community Services (CRCS) Building, 6th & 7th Floor, Jalan Ganesha 10, Bandung 40132, Indonesia, Phone: +62-22-86010080, Fax.: +62-22-86010051; E-mail: jmfs@lppm.itb.ac.id