Electrophoretic Separation of the Soluble Protein of Sclerotinia sclerotiorum, S. trifoliorum and S. Minor

Buddy Dani Kosasih

Abstract


Abstract. Soluble proteins extracted from 3 species of Sclerotinia sclerotiorum, S. trifoliorum and S. minor were separated on polyacrylamide gel electrophoresis. The three species of Sclerotinia could be distinguished by their protein patterns.


Ringkasan. Ekstrak protein dari Sclerotinia sclerotiorum, S. trifiliorum dan S. Minor, sebagai jamur penyebab penyakit sayur-sayuran, telah dapat dipisahkan dengan cara “polyacrylamide gel electrophoresis”. Berdasarkan pola-pola protein yang dibentuk, maka ketiga species tersebut dapat dibedakan, walaupun masih ada beberapa protein yang homolog yang dipunyai oleh ketiga species tersebut.


Full Text:

PDF

References


Chang, L.O., SRB, A.M. and Steward, F.C. 1962. Electrophoretic separation of the soluble proteins of Neurospora. Nature, 193: 756 – 759.

Cheung, D.S.M. and Marshall, K.S. 1969. Antigenic and some kinetic properties of the p-diphenol oxidase isoenzymes of Trameter versicolor. Biochim. Biophys. Acta, 178: 177 – 180.

Clare, B.G. and Zentmeyer, G.A. 1966. Strarch-gel electrophoresis of proteins from species of Phytophthora. Phytopathology, 56: 1334 – 1335.

-----, Flentje, N.T. and Atkinson, M.R. 1968. Electrophoretic patterns of oxidoreductases and other proteins as criteria in fungal taxonomy. Aust. J. Biol. Sci. 21: 275 – 295.

Durbin, R.D. 1966. Comparative gel-electrophoretic investigation of the protein patterns of Septoria species. Nature, 210: 1186 – 1187.

Gill, H.S. and Powell, D. 1968. Polyacrylamide gel (disc) electrophoresis of physiologic races A-1 to A-8 of Phytophtthora fragariae. Phytopathology, 58: 722 – 723.

Glynn, A.N. and J. Reid. 1969. Electrophoretic patterns of soluble fungal proteins and their possible use as taxonomy criteria in the genus Fusarium. Can. J. Bot. 47: 1823 – 1831.

Kulik, M.M. and A.G. Brooks. 1970. Electrophoretic studies of soluble proteins from Aspergillus spp. Mycologia. 62: 365 – 376.

Lowry, O.H., Rosebrough, N.J., Farr, A.L. and Randall, R.J. 1951. Protein measurement with the Folin Phenol Reagent. J. Biol. Chem, 193: 265 – 275.

Macko, V., Honold, G.R. and Stachmann, M.A. 1967, Soluble Proteins and multiple enzyme forms in early growth of wheat Phytochemistry, 6: 465 – 471.

Milton, J.M., Rogers, W.G. and Isaac, I. 1971. Application of acrylamide gel electrophoresis of soluble fungal proteins to taxonomy of Verticillium species. Trans, mycci. Soc. 56 (1): 61 – 65.

Shipton, W.A., and Fleischmann, G. 1969. Taxonomic significance of protein patterns of rust species and formal specialis obtained by disc electrophoresis. Can. J. Bot. 47: 1351 – 1358.

Whitney, P.J., Vaughan, J.G. and Heale, J.B. 1968, A disc electrophoretic study of the proteins of Certicillium albo-atrum, Verticillium dahliae and Fusarium oxysporum with reference to their taxonomy. J. Exptl. Bot. 19: 415 – 426.


Refbacks

  • There are currently no refbacks.


View my Stats

Creative Commons License
This work is licensed under a Creative Commons Attribution-NoDerivatives 4.0 International License.

 

ITB Journal Publisher, LPPM ITB, Center for Research and Community Services (CRCS) Building, 6th & 7th Floor, Jalan Ganesha 10, Bandung 40132, Indonesia, Phone: +62-22-86010080, Fax.: +62-22-86010051; E-mail: jmfs@lppm.itb.ac.id