Paper ID:7112

Optimization of Extraction Method and Characterization of Phycocyanin Pigment from Spirulina platensis

Elin Julianti1, Susanti1, Marlia Singgih1 & Laida Neti Mulyani2

1School of Pharmacy, Institut Teknologi Bandung, Jalan Ganesha 10, Bandung 40132, Indonesia

2Department of Pharmacy, Faculty of Mathematics and Sciences, Sriwijaya University, Jl. Palembang-Indralaya KM 32, Ogan Ilir, South Sumatra, Indonesia

E-mail: elin_julianti@fa.itb.ac.id

Received February 8th, 2018, Revised January 23rd, 2019, Accepted for publication January 24th, 2019

Abstract. Spirulina platensis is a microalgae species that contains phycocyanin, a blue pigment that is widely used in the food, cosmetic and pharmaceutical industries. The objective of this study was to isolate phycocyanin from dried biomass of Spirulina platensis. The optimum extraction was through freeze-thawing extraction with 0.01 M phosphate buffer pH 7. Purification was done using 50% ammonium sulphate precipitation, dialysis, and gel filtration chromatography. The purity ratio of the purified product was 1.729 (A615/A280). Characterization of isolate, carried out using SDS-PAGE, gave molecular weights of around 15-16 Kda and using infrared spectrophotometry the similarity with the standard phycocyanin spectrum was obtained. Both methods confirmed that the isolate was phycocyanin.

Keywords: freeze-thawing; microalgae; phycocyanin; purity ratio; SDS-PAGE; Spirulina platensis.

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