Paper ID:7168

Antimicrobial, Antioxidant and cytotoxic potential of aerial parts of Monotheca buxifolia

Sajjad Ahmad1, Muhammad Nadeem2, Maimoona Gull3, Faiz Ullah Khan4, Muhammad Rafiq1, Ghusia Lutfullah3, Asma Shah3, Hasnain Nangyal5, Zahid Khan6 &Muhammad Saleem Khan7

1Department of microbiology, Quaid-i-Azam University, Islamabad, Pakistan.
2Department of Biotechnology, Quaid-i-Azam University, Islamabad, Pakistan.
3Center of Biotechnology and Microbiology, University of Peshawar, Pakistan
4Department of Pharmacy, Quaid -i-Azam University, Islamabad, Pakistan.
5Department of Botany, Hazara University KPK, Pakistan.
6Faculty of Pharmacy, Federal Urdu University of Arts, Science and Technology Karachi, Pakistan.
7Department of Zoology, Government College University, Faisalabad, Pakistan*
*Corresponding Author: - Muhammad Saleem Khan


Received 14/02/18, Revision 13/12/18, Accept to Publish 14/12/18

Abstract. In this study, different aerial parts of Monotheca Buxifolia were screened for their antibacterial, antifungal, phytotoxic and anti-oxidant activity. Extract of fruits was most active against P. mirabilis, MSSA and M. morganii strains. Moreover, methanolic extract of fruits demonstrated good antibacterial properties against VRSA, S. aureus and Acinetobacter. Despite good antibacterial properties Monotheca buxifolia had low antifungal activity. The pet ether extract of fruit had good cytotoxic, which was most active causing 60% of lethality, with LD50 of 3.307 against Artemia salina larvae. Highest scavenging activity was shown by the methanolic leaves extract in concentration dependent pattern. Findings of this study suggested that Monotheca Buxifolia had good antibacterial, antioxidant potential but  low antifungal properties.

Keywords: antibacterial; antifungal; Antioxidant; cytotoxic; Monotheca, buxifolia


[1]          Kafaru, E., Immense help formative workshop, Essent. Psychopharmacol.,  1, 1994

[2]          Betoni, J.E.C., Mantovani, R.P., Barbosa, L.N., Di Stasi, L.C. & Fernandes Junior, A., Synergism between Plant Extract and Antimicrobial Drugs Used on Staphylococcus Aureus Diseases, Mem. Inst. Oswaldo Cruz, 101(4), pp. 387-390, 2006.

[3]          Lewis, K. & Ausubel, F.M., Prospects for Plant-Derived Antibacterials, Nature Biotechnol, 24(12), pp. 1504-1507, 2006.

[4]          Lee, S.B., Cha, K.H., Kim, S.N., Altantsetseg, S., Shatar, S., Sarangerel, O. & Nho, C.W., The Antimicrobial Activity of Essential Oil from Dracocephalum Foetidum Against Pathogenic Microorganisms, J. Microbiol., 45(1), pp. 53-57, 2007.

[5]          Bonjar, S., Evaluation of Antibacterial Properties of Some Medicinal Plants used in Iran, J. Ethnopharmacol.,  94(2), pp. 301-305, 2004.

[6]          Khan, Novel Anti-Adherence Activity of Mulberry Leaves: Inhibition of Streptococcus Mutans Biofilm by 1-Deoxynojirimycin Isolated from Morus Alba, J. Antimicrob. Chemother., 62(4), pp. 751-757, 2008.

[7]          De Boer, H.J., Kool, A., Broberg, A., Mziray, W.R., Hedberg, I. & Levenfors, J.J., Anti-Fungal and Anti-Bacterial Activity of Some Herbal Remedies from Tanzania, J. Ethnopharmacol., 96(3), pp. 461-469, 2005.

[8]          Khan, N., Ahmed, M., Shaukat, S.S., Wahab, M. & Siddiqui, M.F., Structure, Diversity, and Regeneration Potential of Monotheca Buxifolia (Falc.) A. DC. Dominated Forests of Lower Dir District, Pakistan, Frontiers Agri. China, 5(1), pp. 106-121, 2011.

[9]          Shah, A., Khan Marwat, S., Gohar, F., Khan, A., Hayat Bhatti, K., Amin, M., Ud Din, M., Ahmad, M. & Zafar, M., Ethnobotanical Study of Medicinal Plants of Semi-Tribal Area of Makerwal & Gulla Khel (Lying Between Khyber Pakhtunkhwa and Punjab Provinces), Pakistan. 2013

[10]      Ullah, R., Hussain, Z., Iqbal, Z. & Hussain, I., Traditional Uses of Medicinal Plants in Darra Adam Khel NWFP Pakistan, J. Med. Plants Res., 4(17), pp. 1815-1821, 2010.

[11]      Rehman, J., Khan, I.U., Farid, S., Kamal, S. & Aslam, N., Phytochemical Screening and Evaluation of In-Vitro Antioxidant Potential of Monotheca Buxifolia, E3 J. Biotechnol. Pharm. Res., 4, pp. 54-60, 2013.

[12]      Murad, W., Azizullah, A., Adnan, M., Tariq, A., Khan, K.U., Waheed, S. & Ahmad, A., Ethnobotanical Assessment of Plant Resources of Banda Daud Shah, District Karak, Pakistan,  J. Ethnobiol. Ethnomed., 9(1), pp. 1, 2013.

[13]      Jan, S., Khan, M.R., Rashid, U. & Bokhari, J., Assessment of Antioxidant Potential, Total Phenolics and Flavonoids of Different Solvent Fractions of Monotheca Buxifolia Fruit, Osong Public Health Res. Perspect., 4(5), pp. 246-254, 2013.

[14]      Ullah, I., Khan, J.A., Adhikari, A., Khan, A., Hannan, P.A., Wadood, A. & Farooq, U., Bioassay-guided Isolation of New Urease Inhibitory Constituents from Monotheca buxifolia (Falc.) Fruit and Their Molecular Docking Studies, Rec. Nat. Prod., 10(6), 2016.

[15]      Kuete, V., Tangmouo, J., Beng, V.P., Ngounou, F. & Lontsi, D., Antimicrobial Activity of The Methanolic Extract from The Stem Bark of Tridesmostemon Omphalocarpoides (Sapotaceae), J. Ethnopharmacol,  104(1), pp. 5-11, 2006

[16]      Marwat, S.K., Usman, K., Khakwani, A.A., Ghulam, S., Anwar, N., Sadiq, M. & Khan, S.J., Medico-Ethnobotanical Studies of Edible Wild Fruit Plants Species From the Flora of North Western Pakistan (DI Khan District), J. Med. Plants Res., 5(16), pp. 3679-3686, 2011.

[17]      Shekhawat N, V., Investigation of Anti-Inflammatory, Analgesic and Antipyretic Properties of Madhuca Indica, int. J. Mol. Med. Adv. Sci,  6(2), pp. 26-30., 2010.

[18]      Nasrin, M., Dash, P.R. & Saha, M.R., Investigation of Analgesic and Neuropharmacological Activities of Methanolic Bark Extract of Mimusops Elengi, Int. J. Pharm. Sci. Res., 2(8), pp. 2050, 2011.

[19]      Araujo-Neto, V., R. BomfimI, R., O.B. Oliveira, V., M.P.R. Passos, A., P.R. Oliveira, J., A. Lima, C., S. Mendes, S., S. EstevamI S. & M. Thomazzi, M., Therapeutic Benefits of Sideroxylon Obtusifolium (Humb. Ex Roem. & Schult.) TD Penn., Sapotaceae, in Experimental Models of Pain and Inflammation, Rev. Bras. Farmacogn, 20(6), pp. 933-938, 2010.

[20]      Purnima, A., Koti, B., Thippeswamy, A., Jaji, M., Swamy, A.V., Kurhe, Y. & Sadiq, A.J., Antiinflammatory, Analgesic and Antipyretic Activities of Mimusops Elengi Linn, Indian J. Pharm. Sci., 72(4), pp. 480, 2010.

[21]      Koné, W.M., Vargas, M. & Keiser, J., Anthelmintic Activity of Medicinal Plants Used in Côte d’Ivoire for Treating Parasitic Diseases, Parasitol. Res., 110(6), pp. 2351-2362, 2012.

[22]      Karmakar, U.K., Sultana, R. & Biswas, N.N., Antioxidant, Analgesic and Cytotoxic Activities of Mimusops elengi Linn Leaves, Int. J. Pharm. Sci. Res., 2(11), pp. 2791, 2011.

[23]      Cowan, S.T., Barrow, G., Steel, K.J. & Feltham, R., Cowan and Steel's Manual for the Identification of Medical Bacteria, Cambridge university press, 2004.

[24]      Carron, R., Maran, J., Montero-Fernandozaigo, L. & Dominguez, A., Plantes Medicinales et Phytotherapi, 1987.

[25]      Makhija, I.K., Ram, H.A., Shreedhara, C., Kumar, S.V. & Devkar, R., In Vitro Antioxidant Studies of Sitopaladi Churna, a Polyherbal Ayurvedic Formulation, Free Rad. Antioxidants, 1(2), pp. 37-41, 2011.

[26]      Giustarini, D., Rossi, R., Milzani, A. & Dalle‐Donne, I., Nitrite and Nitrate Measurement by Griess Reagent in Human Plasma: Evaluation of Interferences and Standardization, Methods Enzymol., 440, pp. 361-380, 2008.

[27]      Ullah, R., Ibrar, M., Shah, S. & Hameed, I., Phytotoxic, Cytotoxic and Insecticidal Activities of Calendula Arvensis L, J. Biotechnol. Pharm. Res., 3(6), pp. 104-11, 2012.

[28]      Diastuti, H., Syah, Y.M., Juliawaty, L.D. & Singgih, M., Antibacterial Curcuma Xanthorrhiza Extract and Fractions, J. Math. Fund. Sci., 46(3), pp. 224-234, 2014.

[29]      Adnan, M., Bibi, R., Mussarat, S., Tariq, A., & Shinwari, Z.K., Ethnomedicinal and Phytochemical Review of Pakistani Medicinal Plants Used as Antibacterial Agents Against Escherichia Coli, Ann. Clin. Microbiol. Antimicrob., 13(1), pp. 1, 2014.

[30]      Ahmad, I. & Beg, A.Z., Antimicrobial and Phytochemical Studies on 45 Indian Medicinal Plants against Multi-Drug Resistant Human Pathogens, J. Ethnopharmacol., 74(2), pp. 113-123, 2001.

[31]      Irobi, O. & Daramola, S., Bactericidal Properties of Crude Extracts of Mitracarpus Villosus, J. Ethnopharmacol., 42(1), pp. 39-43, 1994.

[32]      Tsuchiya, H., Sato, M., Miyazaki, T., Fujiwara, S., Tanigaki, S., Ohyama, M., Tanaka, T. & Iinuma, M., Comparative Study on the Antibacterial Activity of Phytochemical Flavanones Against Methicillin-Resistant Staphylococcus Aureus, J. Ethnopharmacol., 50(1), pp. 27-34, 1996.

[33]      Jesonbabu, J., Spandana, N. & Lakshmi, K.A., In Vitro Antimicrobial Potentialities of Chloroform Extracts of Ethanomedicinal Plant against Clinically Isolated Human Pathogens, Int. J. Pharm. Pharm. Sci, 4(3), pp. 624-626, 2012.

[34]      Valle, D.L., Andrade, J.I., Puzon, J.J.M., Cabrera, E.C. & Rivera, W.L., Antibacterial Activities of Ethanol Extracts of Philippine Medicinal Plants Against Multidrug-Resistant Bacteria, Asian Pac. J. Trop. Biomed., 5(7), pp. 532-540, 2015.

[35]      Rath, S. & Padhy, R.N., Antibacterial Efficacy of Five Medicinal Plants against Multidrug-Resistant Enteropathogenic Bacteria Infecting Under-5 Hospitalized Children, J. Integr. Med., 13(1), pp. 45-57, 2015.

[36]      Cowan, M.M., Plant Products as Antimicrobial Agents, Clin. Microbiol. Rev., 12(4), pp. 564-582, 1999.

[37]      Noumedem, J.A., Mihasan, M., Lacmata, S.T., Stefan, M., Kuiate, J.R. & Kuete, V., Antibacterial Activities of the Methanol Extracts of Ten Cameroonian Vegetables against Gram-Negative Multidrug-Resistant Bacteria, BMC Complement Altern. Med., 13(1), pp. 1, 2013.

[38]      Nair, R. & Chanda, S., Activity of Some Medicinal Plants against Certain Pathogenic Bacterial Strains, Indian J. Pharmacol., 38(2), pp. 142, 2006.

[39]      Mahesh, B. & Satish, S., Antimicrobial Activity of Some Important Medicinal Plant against Plant and Human Pathogens, World J. Agri. Sci., 4(5), pp. 839-843, 2008.

[40]      Mahmoud, D., Hassanein, N., Youssef, K. & Zeid, A., Antifungal Activity of Different Neem Leaf Extracts and The Nimonol Against Some Important Human Pathogens, Braz. J. Microbiol., 42(3), pp. 1007-1016, 2011.

[41]      Kelly, F.J., Use of Antioxidants in the Prevention and Treatment of Disease, J. Int. Fed. Clin. Chem., 10(1), pp. 21-3, 1998.

[42]      Pellati, F., Benvenuti, S., Magro, L., Melegari, M. & Soragni, F., Analysis of Phenolic Compounds and Radical Scavenging Activity of Echinacea SPP. J. Pharm. Biomed. Anal., 35(2), pp. 289-301, 2004.

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