Paper ID:8823

Cytotoxic Activities of Extracts and Isolated Compounds of Some Potential Sumatran Medicinal Plants against MCF-7 and HSC-3 Cell Lines

Meiridha Mutiara Andania1, Friardi Ismed1, Muhammad Taher2, Solachuddin Jauhari Arief Ichwan3, Amri Bakhtiar1 & Dayar Arbain1

1Faculty of Pharmacy/Sumatran Biota Laboratory, Andalas University, Padang 25163, West Sumatra, Indonesia

2Department of Pharmaceutical Technology, Faculty of Pharmacy, International Islamic University Malaysia, 25200, Kuantan, Pahang, Malaysia

3Department of Basic Medical Sciences, Faculty of Dentistry, International Islamic University Malaysia, 25200, Kuantan, Pahang, Malaysia

E-mail: d.arbain@phar.unand.ac.id

Received September 19th, 2018, Revised March 11th, 2019, Accepted for publication April 5th, 2019.

Abstract. In continuation of our study of Sumatran plants and the bioactivity of their constituents, Stereocaulon halei Lamb (Stereocaulaceae) was extracted and isolated to obtain atranorin (1), lobaric acid (6), and methyl-β-orcinol carboxylate (9). We also prepared methanolic extracts of some Sumatran medicinal plants, i.e. Alpinia denticulata, Alpinia malaccensis, Alpinia submutica, Curcuma zedoaria, Centella asiatia, Mangifera indica, Piper crocatum, Mussaenda frondosa, together with previously isolated compounds, i.e. crocatin A (2) and crocatin B (3) from P. crocatum, curcumin (4) from Curcuma longa, demethoxymatteucinol (5) from Sphaerostephanos polycarpus, mangiferin (7) from M. indica, methyl caffeate (8) from Balanophora elongata, tiliroside (10) from Guazuma ulmifolia, and usnic acid from Usnea sp (11). These extracts and compounds were tested for their cytotoxicity against MCF-7 and HSC-3 cell lines by MTT assay (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide). It was found that the IC50 value of atranorin (1), lobaric acid (6), methyl-β-orcinol carboxylate (9), and methanolic extract of A. submutica towards the MCF-7 cell line was 208.20 µM, 172.05 µM, 382.60 µM and 70.95 µg/mL, respectively. The IC50 value of lobaric acid (6) and methyl-β-orcinol carboxylate (9) against the HSC-3 cell line was 88.92 µM and 260.09 µM, respectively. The other extracts did not show significant cytotoxicity.

Keywords: cytotoxic; extracts; isolated compounds; MCF-7, HSC-3; Sumatran medicinal plants.

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