Paper ID:9078

Effects of Root Extracts of Ruellia tuberosa L. on Kidneys of Diabetic Rats

Anna Safitri,1,3* Arie Srihardyastutie,1 Anna Roosdiana,1 Aulanni’am,1,2 Eva Nur Laili Octaviana,1

1Chemistry Department, Brawijaya University, Malang, 65145
2Faculty of Veterinary Medicine, Brawijaya University, Malang, 65145
3Research Center for Smart Molecules of Natural Genetic Resources (SMONAGENES), Brawijaya University, Malang 65145


Received, September 25th, 2017, Revised 1 November 20th, 2017, Accepted 22 November 2018

Abstract. The aim of this research is to study antidiabetic activity of root extract of Ruellia tuberosa L. on rats (Rattus novergicus) induced multiple-low dose streptozotocin. The parameters investigated were blood glucose levels, protease activities, and histopathologic profiles on the kidneys. Three groups of rats, including control, diabetic, and treatment groups were used. The R. tuberosa L. extracts were given orally for treatment in the dose of 250 mg/kg body weight per day for 21 consecutive days. The induced diabetes group treated with root extracts of R. tuberosa L. (group III) had 60.3% lower glucose levels (119 mg/dL) compared to the group with induced diabetes alone. Moreover, the antidiabetic activity of R. tuberosa L extracts were evidenced by the 55% lower protease activity in the treatment group, compared to the untreated diabetic group. Finally, a positive effect from root extract of R. tuberosa L. was shown from the less damaged renal glomerulus and smaller gap between extracellular matrices and epithelium cells in histopathologic profiles of the kidneys’ in the extract treatment group. These results show the potential of root extract of R. tuberosa L. as a natural product anti-diabetic agent.

Keywords: diabetes, diabetic rats, kidneys, protease activities, Ruellia tuberosa L.


 [1]               Guthrie, R. A. & Guthrie, D. W. Pathophysiology of diabetes mellitus, Crit. Care Nursing Quart., 27, pp. 113-125, 2004.

[2]               Paneni, F., Beckman, J. A., Creager, M. A., Cosentino, F., Diabetes and vascular disease: pathophysiology, clinical consequences, and medical therapy: part I, Eur. Heart J., 32, pp. 2436-2443, 2013.

[3]               Gupta, R. K., Diabetes: A Forecast for 2030. Int. J. Eng. Tech. Science Res.4, pp. 93-97,  2017.

[4]               Ministry of Health Republic of Indonesia, Diabetic in Indonesia, Jakarta, Vol 2017, 2009.

[5]               Bell, D. S. H., Combine and conquer: advantages and disadvantages of fixed-dose combination therapy, Diabet. Obes. Metab. 15, pp. 291-300, 2012.

[6]               Herman, W. H, in Diabetes Mellitus in Developing Countries and Underserved Communities, Dagogo-Jack, S., Ed.; Springer International Publishing, 2016.

[7]               Rao, M. U., Sreenivasulu, M., Chengaiah, B., Reddy, K. J., Chetty, C. M, Herbal medicines for diabetes mellitus: A review. Int. J. PharmTech Res., 2, pp.1883-1892,  2010.

[8]               Modak, M, Dixit, P., Londhe, J., Saroj, G., Ghaskadbi, Devasagayam, T. P. A., 2007. Indian Herbs and Herbal Drugs Used for the Treatment of Diabetes, J. Clin. Biochem. Nutr., 40, pp. 163–173, 2007.

[9]               Manikandan, A., Victor, A., Doss, A., Effect of 50% hydroethanolic leaf extracts of Ruellia tuberosa L. and Dipteracanthus patulus (Jacq.) on non-enzymic antioxidants and other biochemical parameters in liver, kidney, serum of alloxan induced diabetic Swiss albino rats, J. Biomed. Sci. Res., 2, pp. 190-201, 2010.

[10]           Manikandan, A., Victor, A., Doss, A., Evaluation of biochemical contents, nutritional value, trace elements, SDS-PAGE, and HPTLC profiling in the leaves Ruellia tuberosa L and Dipteracanthus patulus (Jacq). J. Chem. Pharm. Res., 2, pp. 295-303, 2010.

[11]           Pitocco, D., Tesauro, M., Alessandro, R., Ghirlanda, G., Cardillo, C., Oxidative stress in diabetes: implications for vascular and other complications, Int. J. Mol. Sci., 14, pp. 21525-21550, 2013.

[12]           Rochete, L., Zeller, M., Cottin, Y., Vergely, C., Diabetes, oxidative stress and therapeutic strategis., Biochim. Biophys. Acta, 1840, pp. 2709-2729, 2014.

[13]           Martin, C. L., Albers, J. W., Pop-Busui, R., Neuropathy and related findings in the diabetes control and complications trial/epidemiology of diabetes intervention and complications study, Diabet. Care, 37, pp. 31-38, 2014.

[14]           de Broer, I. H., Kidney disease and related findings in the diabetes control and complications trial/epidemiology of diabetes interventions and complication study, Diabet. Care, 37, pp. 24-30, 2014.

[15]           Sting, C., Soderquist, M., Karlsson, O., Boren, M., Luider, T. M., Uncovering effects of ex vivo protease activity during proteomics and and peptidomics sample extraction in rat brain tissue by oxygen-18 labelling, J. Proteom. Res, 13, 2807-2817, 2014.

[16]           Origin version 6.1, Microcal origin Inc., Northampton, MA, USA, 1999.

[17]           Wang, Z., Yang, Y., Xiang, X., Zhu, Y., Men, J., He, M., Estimation of the normal range of blood glucose in rats, J. Hygiene Res., 39, pp. 133-137, 2010.

[18]           Musante, L., Tataruch, D., Gu, D., Liu, X., Forsblom, C., Groop, P.-H., Holthofer, H., Protease and protease inhibitors of urinary extracellular vesicles in diabetic nephropathy, J. Diabet. Res., 2015, 14 pages.

[19]           Andersen, H., Friis, U. G., Hanse, P. B., L., Svenningsen, H. P., Henriksen, J. E., Jensen, B. L., Diabetic nephropathy is associated with increased urine excretion of proteases plasmin, prostasin, and urokinase and activation of amiloride-sensitive current in collecting duct cells, Nephr. Dialysis Transplant., 30, pp 781-798, 2015.

[20]           Bi, L., Tian, X., Dou, F., Hong, L., Tang, H., Wang, S., New antioxidant and antiglycation active triterpenoid saponins from the root bark of Aralia taibaiensis, Fitoterapia, 83, 234-240, 2012.

[21]           Eom, H. J., Kang, H. R., Kim, H. K., Jung, E. B., Park, H. B., Kang, K. S., Bioactivy-guided isolation of antioxidants triterpenoids from Betula platypylla var japonica bark, Bioorg. Chem., 66, 97-101, 2016.

[22]           Grove, K. J., Voziyan, P. A., Spraggins, J. M., Wang, S., Paueksakon, P., Harris, R. C., Hudson, B. G., Caprioli, R. M., Diabetic nephropathy induces alterations in the glomerular and tubule lipid profiles, J. Lipid Res., 55, pp. 1375-1385, 2014.

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