Paper ID:9078

Effects of Root Extracts of Ruellia tuberosa L. on Kidneys of Diabetic Rats

Anna Safitri,1,3* Arie Srihardyastutie,1 Anna Roosdiana,1 Aulanni’am,1,2 Eva Nur Laili Octaviana,1

1Chemistry Department, Brawijaya University, Malang, 65145
2Faculty of Veterinary Medicine, Brawijaya University, Malang, 65145
3Research Center for Smart Molecules of Natural Genetic Resources (SMONAGENES), Brawijaya University, Malang 65145

Email: a.safitri@ub.ac.id

Received, September 25th, 2017, Revised 1 November 20th, 2017, Accepted 22 November 2018

Abstract. The aim of this research is to study antidiabetic activity of root extract of Ruellia tuberosa L. on rats (Rattus novergicus) induced multiple-low dose streptozotocin. The parameters investigated were blood glucose levels, protease activities, and histopathologic profiles on the kidneys. Three groups of rats, including control, diabetic, and treatment groups were used. The R. tuberosa L. extracts were given orally for treatment in the dose of 250 mg/kg body weight per day for 21 consecutive days. The induced diabetes group treated with root extracts of R. tuberosa L. (group III) had 60.3% lower glucose levels (119 mg/dL) compared to the group with induced diabetes alone. Moreover, the antidiabetic activity of R. tuberosa L extracts were evidenced by the 55% lower protease activity in the treatment group, compared to the untreated diabetic group. Finally, a positive effect from root extract of R. tuberosa L. was shown from the less damaged renal glomerulus and smaller gap between extracellular matrices and epithelium cells in histopathologic profiles of the kidneys’ in the extract treatment group. These results show the potential of root extract of R. tuberosa L. as a natural product anti-diabetic agent.

Keywords: diabetes, diabetic rats, kidneys, protease activities, Ruellia tuberosa L.

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