3BIO: Journal of Biological Science, Technology and Management 2020-10-06T03:39:21+07:00 Dr. Rudi Dungani Open Journal Systems <p><em><img class="imgdesc" src="" alt="" /></em></p> <p><em><strong>3BIO</strong></em> provides a venue to promote scientific discourse and foster scientific developments related to bio-sciences, bio-technology and bio-management.</p> <p><br /><em><strong>3BIO</strong></em> publishes articles discussing advances in the science, technology and management within the scope of basic and applied biological science. Emphasis is placed on a wide spectrum of topics, from genetic or molecular biological positions to those covering biochemical, chemical or bioprocess engineering aspects as well as all aspects of management related to strategy, entrepreneurship, innovation, information technology, and organizations as well as all functional areas of business, such as finance, marketing, and operations, provided that in each case the material is directly relevant to biological systems.</p> <p> </p> <p>To submit an article, please make an online submission by registering to this website (<a title="Registration page" href="" target="_blank" rel="noopener">registration link</a>) or login to your account. Click "author" and follow steps to start a new submission. The submitted manuscript must follow the 3BIO template. Download the Submission Guidelines and Template on the <a title="Submission Guidelines" href="" target="_blank" rel="noopener">Submission menu</a>.</p> <p>e-ISSN: 2655-8777</p> <p><a href="">View My Stats</a></p> The Protective Effect of Propolis Nanoemulsion (NEP) Against UVB Irradiation Induced Photoaging in Human Dermal Fibroblast (HDF) 2020-10-06T03:39:17+07:00 Anggraini Barlian Rini Agustin Ramadhani Eka Putra <div><p class="IOPKwd"><span lang="EN-US">The skin damage induced by UV irradiation can cause photoaging as a consequence of reactive oxygen species (ROS) generation. Propolis is one of the most abundant natural product sources of polyphenols and derived from various plant resins collected by honeybees. This study aimed to determine the due administration of the propolis nanoemulsion (NEP) as an anti-photoaging by observing the concentration of intracellular ROS and lipid peroxides in human dermal fibroblast (HDF) by exposure to UVB. The cytotoxic of NEP was tested by the MTT assay, intracellular ROS was measured using the DCFDA assay, lipid peroxide products were analyzed by the TBARS assay and cell nuclei was observed by DAPI staining. </span><span lang="HR">PSA analysis revealed that the sizes of NEP were about 131.9 to 131 nm</span><span lang="EN-US">. The optimum concentration of NEP was 0,1μg / ml. ROS production in HDF treatment of NEP significantly decreases (p &lt;0.05). Lipid peroxide products showed the same pattern as ROS concentration. The lipid peroxide concentration in the treatment NEP significantly decreases (p&lt;0.05). In conclusion, the propolis in NEP acts as an antioxidant and has the potential to reduce the production of ROS and lipid peroxides caused by exposure to UVB.</span></p></div> 2020-10-05T00:00:00+07:00 Copyright (c) Aging Process in Dermal Fibroblast Cell Culture of Green Turtle (Chelonia mydas) 2020-10-06T03:39:18+07:00 Anggraini Barlian Yemima D Riani <div><p class="IOPKwd"><span lang="EN-US">Senescence in green turtle is an interesting process to study because until now no clear explanation has been established about senescence at cellular or molecular level in this species. One of common markers used for detecting senescence is telomere shortening. Reduced telomerase activity may also cause senescence. The aims of this research are to establish and identify dermal fibroblast cell culture from green turtle and also to compare telomere length and telomerase activity from the cells subculture 5 and 14. Cells were identified with Rabbit Anti-Vimentin Polyclonal Antibody and Goat Polyclonal Antibody using confocal microscope. Telomere length was obtained using TeloTAGGG Telomere Length Assay (Roche), while telomerase activity was obtained using TeloTAGGG Telomerase PCR ElisaPlus. Primary cell culture from green turtle skin showed fibroblastic morphology and immunocytochemistry results using vimentin antibody proved that the culture was fibroblast cell. Measurement of telomere length and telomerase activity showed that telomere length and telomerase activity of subculture 14 was greater than subculture 5. However, based on morphology, green turtle fibroblast skin cell culture showed senescent morphology. Therefore, possible aging mechanism that the green turtle fibroblast skin cell culture underwent did not go through both telomere shortening and reduced telomerase activity.</span></p></div> 2020-10-05T00:00:00+07:00 Copyright (c) Production of Bioethanol and Crude Cellulase Enyzme Extract from Napier Grass (Pennisetum purpureum S.) through Simultaneous Saccharification and Fermentation 2020-10-06T03:39:19+07:00 Taufikurahman Taufikurahman Sherly - Xie <div><p class="IOPKwd"><span lang="HR">Napier grass (<em>Pennisetum purpureum </em>S.) has been recognized for its high amounts of cellulose and hemicellulose which can be utilized for bioethanol production. Bioconversion of Napier grass to bioethanol can be performed by filamentous fungi. A fungi, <em>Neurospora sitophila</em>, can synthesize and secrete hydrolytic enzymes to breakdown the cellulose and hemicellulose into various monomeric sugars and simultaneously convert it to bioethanol. This study investigated the effect of Napier grass substrate concentrations and cultivation time on cellulase enzyme and bioethanol production by <em>N. sitophila</em>. The pretreatment of Napier grass was carried out using 1.5% (w/v) NaOH solution for 120 hours at 30 °C. After the pretreatment process, simultaneous saccharification and fermentation was conducted at 30 °C; pH 6.8-7.0 and agitation speed 130 Rpm with various of Napier grass substrate concentrations (10, 20 and 30 g l<sup>-1</sup>) and cultivation period within 24; 48; 72; 96 and 120 hours. The results show that the highest enzyme activity is 0.28 FPU/ml which was achieved at 120 hours of cultivation and 20 g/L substrate concentration of Napier grass. The highest ethanol content was obtained by 96 hours of simultaneous saccharification and fermentation (1.25 g l<sup>-1</sup>) using 20 g l<sup>-1</sup> substrate concentration and the maximum ethanol yield is 0.30 g/g cellulose conducted at 30 g l<sup>-1</sup> substrate concentration and 96 hours of fermentation.</span></p></div> 2020-10-05T00:00:00+07:00 Copyright (c) Increase of Silica Recovery by Lignocellulose-degrading Enzyme Complex from Cow Rumen Fluid in a Reactive Extraction of Oryza sativa L. Husk 2020-10-06T03:39:19+07:00 Muhammad Rizki Fajar Ilhamy Khairul Hadi Burhan Robert Manurung <span lang="EN-GB">Silica is one of the beneficial nutrients and has various essential roles to cope with stresses situation in the graminaceous plant. The availability of Si on earth is abundant however the form that can be readily uptake by the plant is limited, and without external addition, the silica content in the cultivated land would be depleted and decreasing plant growth, recycle Si from the decaying plant is a better alternative to protect from that situation. Rice husk is a leftover paddy by-product having significant Si content that can be reused. This study offered a biological way to treat the rice husk using rumen fluid as a better alternative to the high energy consuming thermal procedure. Rice husk was macerated in rumen fluid, the kinetic and model of lignocellulose degradation were evaluated. The study in Si release in liquid media i.e. 0.05 M hydrochloric acid, 0.1 citric acid and aquades, and Si extraction in alkaline solution 1 M KOH using the rumen-treated husk showed that this method could be a better alternative to develop high Si content of solid and liquid fertilizer, respectively.</span> 2020-10-05T00:00:00+07:00 Copyright (c) Risk Assesment and Management Recommendations of Invasive Species in Papandayan Mountain Nature Reserve, West Java 2020-10-06T03:39:20+07:00 Anggi Sella Nyuanti Rina Ratnasih Irwanto Elham Sumarga <div><p class="IOPKwd"><span lang="HR">Natural disasters and anthropogenic disturbances have caused several locations in the Papandayan Mountain Nature Reserve to be more vulnerable and exposed further triggering the presence of invasive species. This presence impact</span><span lang="IN">s</span><span lang="HR"> the environment, the economy, and the health of humans and animals. This research aims to assess the risks of these invasive species, followed by various recommendation strategies towards their species management. </span><span lang="IN">Data</span><span lang="HR"> on species diversity </span><span lang="IN">was collected</span><span lang="HR"> by vegetation analysis, using the quadratic method by </span><span lang="IN">purposively </span><span lang="HR">sampling plot</span><span lang="IN">s</span><span lang="HR">. Results showed the existence of six invasive species in Papandayan i.e. </span><span lang="EN-GB"><a href=""><em><span lang="HR">Ageratina riparia </span></em><span lang="HR">(Regel) R.M.King &amp; H.Rob</span></a></span><span lang="HR">.<em>, </em></span><span lang="EN-GB"><a href=""><em><span lang="HR">Ageratina adenophora </span></em><span lang="HR">(Spreng.) R.M.King &amp; H.Rob.,</span></a></span><em></em><span lang="EN-GB"><a href=""><em><span lang="HR">Austroeupatorium</span></em><span lang="HR"> <em>inulaefolium</em> (Kunth) R.M.King &amp; H.Rob</span></a></span><span lang="HR">.<em>, </em></span><span lang="EN-GB"><a href=""><em><span lang="HR">Imperata</span></em><span lang="HR"> <em>cylindrica</em> (L.) Raeusch.</span></a></span><span lang="HR">,<em> </em></span><span lang="EN-GB"><a href=""><em><span lang="HR">Rubus</span></em><span lang="HR"> <em>moluccanus</em> L.</span></a></span><span lang="HR">, and<em> </em></span><span lang="EN-GB"><a href=""><em><span lang="HR">Ageratum</span></em><span lang="HR"> <em>conyzoides</em> (L.) L.</span></a></span><em><span lang="HR">. </span></em><span lang="HR">The assessment of<em> </em>i</span><span lang="HR">nvasive species was assessed through two indices; Risk Index and Feasibility Index. Each of them was calculated based on the Risk Assessment Protocol to determine their strategy of control. Based on the Risk and Feasibility Indices, several recommendation strategies to manage invasive species are proposed.</span></p></div> 2020-10-05T00:00:00+07:00 Copyright (c)