https://journals.itb.ac.id/index.php/acta/issue/feed Acta Pharmaceutica Indonesia 2021-06-30T18:18:11+07:00 Dr. Kusnandar Anggadireja actapharm_id@fa.itb.ac.id Open Journal Systems <a href="https://journals.itb.ac.id/index.php/acta"><img class="imgdesc" src="https://journals.itb.ac.id/public/site/images/admin_acta/cover_issn.jpg" alt="" /></a><p style="text-align: justify;">Acta Pharmaceutica Indonesia merupakan jurnal resmi yang dipublikasikan oleh Sekolah Farmasi Institut Teknologi Bandung. Jurnal ini mencakup seluruh aspek ilmu farmasi sebagai berikut (namun tidak terbatas pada): farmasetika, kimia farmasi, biologi farmasi, bioteknologi farmasi, serta farmakologi dan farmasi klinik.</p><p style="text-align: justify;">Acta Pharmaceutica Indonesia is the official journal published by School of Pharmacy Institut Teknologi Bandung. The journal covers all aspects of pharmaceutical issues which includes these following topics (but not limited to): pharmaceutics, pharmaceutical chemistry, biological pharmacy, pharmaceutical biotechnology, pharmacology and clinical pharmacy.</p><p>ISSN: 0216-616X (printed); 2776-0219 (online)</p><p></p> https://journals.itb.ac.id/index.php/acta/article/view/16629 Front Matter Vol 46 No 1 (2021) 2021-06-30T17:06:50+07:00 Acta Pharmaceutica Indonesia actapharm_id@fa.itb.ac.id 2021-06-30T00:00:00+07:00 Copyright (c) 2021 Acta Pharmaceutica Indonesia https://journals.itb.ac.id/index.php/acta/article/view/14917 Development and Validation of Spectrofluorometric Method for the Determination of Ascorbic Acid in Several Dosage Forms by Using Methylene Blue 2021-01-14T04:27:44+07:00 Benny Permana BennyPer12345@gmail.com Debora Ronauli benny_permana@fa.itb.ac.id Ilma Nugrahani ilma_nugrahani@fa.itb.ac.id <p>Ascorbic acid or vitamin C is one of the most widely consumed supplement and available at the market in several preparations such as tablet, sweetlet, effervescent tablet, and ready to drink product. Spectrofluorometric method has been developed in this study for quantitative determination of ascorbic acid in various forms of products based on the reaction between ascorbic acid and methylene blue. Methylene blue concentration decreased along with the addition of ascorbic acid due to a redox reaction which caused the formation of colorless leuco-methylene blue and dehydroascorbic acid. The fluorescence intensity of methylene blue was measured at excitation and emission wavelengths of 664 nm and 686 nm respectively. The proposed method was found to have a good selectivity, linearity, accuracy, and precision. Validation studies demonstrated a good linearity for the method with a correlation coefficient &gt; 0.999 and Vx0 &lt; 2%. The accuracy test also met the requirements with the recoveries were not less than 90.0% and not more than 110.0%. Precision test gave a relative standard deviation (% RSD) not more than 2%. The ascorbic acid content in several marketed products was evaluated by this new spectrofluorometric method and the result was in good agreement for effervescent tablet, isotonic drink and lozenges with the label claim and specification. Due to its simplicity, the method can be used to analyze vitamin C in different product forms.</p> 2021-06-30T00:00:00+07:00 Copyright (c) 2021 Acta Pharmaceutica Indonesia https://journals.itb.ac.id/index.php/acta/article/view/14915 NMR Study of Methyl β-orsellinate from Dirinaria sp. was Collected from Coastal Areas of Teluk Nipah, Pulau Pangkor, Perak Malaysia 2021-01-18T02:06:59+07:00 Andi Rifki Rosandy andirifki914@gmail.com Nurul Nadiah Binti Abdul Rahman rifqi@bbrc.itb.ac.id Rozida Khalid rifqi@bbrc.itb.ac.id Agus Chahyadi rifqi@bbrc.itb.ac.id Elfahmi Elfahmi elfahmi@fa.itb.ac.id <p>The research was carried out to isolate and characterize the methyl <em>β</em>-orsellinate of lichen <em>Dirinaria</em> sp. collected from the coastal areas of Teluk Nipah, Pulau Pangkor, Perak Malaysia. The sample was extracted in methanol for three times at room temperature. Subsequently, the crude extracts and further analysed using thin-layer chromatography (TLC) and vacuum liquid chromatography (VLC). To derive the pure compounds, the isolation step was performed using radial chromatography (RC). The chemical structure of the isolated compound was determined by several spectroscopies i.e. infra-red (IR), nuclear magnetic resonances (NMR), and mass spectrometric (MS). The compound was identified as methyl <em>β</em>-orsellinate.</p> <p> </p> 2021-06-30T00:00:00+07:00 Copyright (c) 2021 Acta Pharmaceutica Indonesia https://journals.itb.ac.id/index.php/acta/article/view/13052 Identifikasi Mikroba dan Analisis Kandungan Gizi dari Bahan Pangan Tradisional Gatot dan Modifikasinya 2020-12-11T09:09:54+07:00 Imas Ambarsari aleedaria@gmail.com Marlia Singgih Wibowo marlia@fa.itb.ac.id Rahmana Emran Kartasasmita kartasasmita@fa.itb.ac.id <p>Gatot merupakan pangan olahan tradisional dari singkong yang mempunyai potensi sebagai bahan pangan pengganti, namun kualitas hasil produksi dari industri kecil sering kali kurang terjamin dan berpotensi merugikan kesehatan. Penelitian ini bertujuan untuk mengidentifikasi mikroba dan nutrisi yang terdapat dalam gatot tradisional serta serta membandingkannya dengan gatot modifikasi yang dibuat sendiri. Gatot modifikasi dibuat melalui fermentasi fase padat menggunakan pembungkus yang berbeda yaitu plastik, daun pisang dan kain. Hanya gatot modifikasi yang difermentasi dalam plastik yang menunjukkan hasil yang mendekati dengan gatot yang diproduksi di daerah asli yaitu Pacitan, dengan warna hitam pada bagian dalam dan luar, tekstur kenyal, aroma khas tidak menyengat, dan rasa gurih. Terdapat pertumbuhan <em>Aspergillus </em><em>flavus</em> dan <em>Aspergillus niger</em>, <em>Pseudomonas aeruginosa </em>dan bakteri asam laktat <em>Pediococcus sp </em>pada gatot tradisional. Sampel gatot modifikasi yang difermentasi di dalam plastik teridentifikasi mengandung bakteri asam laktat <em>Pediococcus sp </em>dan ragi. Sedangkan sampel yang difermentasi di dalam daun pisang mengandung <em>A. flavus</em> dan <em>Saccharomyces cerevicae</em> dan Gatot yang difermentasi di dalam kain mengandung <em>A. niger. </em>Gatot hasil fermentasi dalam plastik memiliki kadar protein paling tinggi dibandingkan sampel lain yaitu sebesar 3,8 %, meningkat jika dibandingkan kadar protein singkong mentah. Logam berat Pb dan Cd tidak ditemukan pada semua sampel gatot. Kandungan Fe ditemukan dengan kadar sebesar 8,19 ppm pada gatot industri tradisional sedangkan gatot modifikasi memiliki kadar besi yang sangat rendah.</p> <p> </p> 2021-06-30T00:00:00+07:00 Copyright (c) 2021 Acta Pharmaceutica Indonesia https://journals.itb.ac.id/index.php/acta/article/view/16264 Role of the Arachidonic Acid Cascade in the Expression of Withdrawal Signs in Alcohol Dependent Mice 2021-05-07T14:59:30+07:00 Kusnandar Anggadireja kusnandar@fa.itb.ac.id Razana Bt. Adam Lee kusnandar@fa.itb.ac.id <p>Withdrawal symptom in alcohol dependence is one the main problems in treating alcohol addiction. This study was aimed to clarify the role of the arachidonic acid cascade in the expression of withdrawal signs in animal model of alcohol dependence. Dependence was induced by repeated administration of 15% ethanol in 0.2% sucrose solution for 17 days. On the 18<sup>th</sup> day, the ethanol was not given, and one hour after the supposed time of ethanol administration, diclofenac at 10 mg/kg was injected i.p. to induce withdrawal. Observation of behavioral withdrawal signs was performed for 30 minutes, starting 25 minutes after diclofenac injection. Following behavioral observation, forced swimming test ensued to assess depression during withdrawal. In different group of mice, ibuprofen at 7.5 mg/kg was given every other day during ethanol regimen, 30 minutes prior to ethanol. Results showed that diclofenac induced the expression of withdrawal signs in mice receiving repeated alcohol. Incidences of facial preening (P&lt;0.01), forepaw licking (P&lt;0.01), grooming (P&lt;0.01) and forepaw tremor (P&lt;0.001) increased significantly by at least three times the normal value. In mice pretreated with ibuprofen significant decreases in body weight reduction (300%, P&lt;0.01) and immobility time (50%, P&lt;0.05) were observed compared to that of ethanol group. Taken together, the data indicates that suppressed arachidonic acid cascade signaling is involved in the expression of alcohol withdrawal.</p> 2021-06-30T00:00:00+07:00 Copyright (c) 2021 Acta Pharmaceutica Indonesia https://journals.itb.ac.id/index.php/acta/article/view/12330 Perbedaan Pola Makan yang Mengandung Vitamin D Terkait Latar Belakang Pendidikan Mahasiswa Farmasi dengan Nonfarmasi 2021-01-14T04:40:04+07:00 Amelia Lorensia amelia.lorensia@gmail.com Dian Natasya Raharjo amelia.lorensia@gmail.com Sri Rahayu amelia.lorensia@gmail.com <p>Defisiensi vitamin D sering dikaitkan dengan beberapa penyakit kronis. Indonesia merupakan Negara tropis yang sepanjang tahun disinari matahari. Sinar matahari merupakan sumber terbesar vitamin D, tetapi defisiensi vitamin D masih banyak dialami di negara tropis . Sumber utama lain vitamin D merupakan makanan, perilaku konsumsi makanan yang salah pada masa remaja menyebabkan ketidakseimbangan antara konsumsi gizi dengan kecukupan gizi. Penelitian ini dilakukan untuk analisis perbedaan asupan dan faktor resiko defisiensi vitamin D dengan menggunakan metode <em>cross sectional </em>pada 100 mahasiswa farmasi dan 100 mahasiswa nonfarmasi di suatu unviersitas di Surabaya. Metode pengambilan menggunakan <em>Food Frequency Questionnaire</em> (FFQ). Hasil penelitian menunjukkan nilai p yang didapat adalah sebesar 0,568 (nilai p&gt;0,05) Ho diterima sehingga dapat dikatakam bahwa tidak ada perbedaan asupan makanan yang mengandung vitamin D pada mahasiswa farmasi dan nonfarmasi. Selain itu didapatkan nilai POR 1,244 yang dapat disimpulkan asupan makanan terkait resiko defisiensi vitamin D memiliki kecenderungan resiko yang sama pada kedua kelompok responden (farmasi dan nonfarmasi ). Oleh karena itu, diperlukan peningkatan kesadaran yang lebih tinggi pada mahasiswa farmasi sebagai calon apoteker yang merupakan tenaga kesehatan.</p> 2021-06-30T00:00:00+07:00 Copyright (c) 2021 Acta Pharmaceutica Indonesia