Development of Gold Nanoparticle (AuNP)-based Colorimetric Aptasensor for Penicillin G Detection

Authors

  • Darmawati Darmawati Department of Chemistry, Faculty of Mathematics and Natural Science, Universitas Indonesia, Depok, 16424, Indonesia
  • Apon Zaenal Mustopa Research Center for Genetic Engineering, National Research and Innovation Agency (BRIN), Bogor, 16911, West Java, Indonesia
  • Bugi Ratno Budiarto Research Center for Genetic Engineering, National Research and Innovation Agency (BRIN), Bogor, 16911, West Java, Indonesia
  • Siti Irma Rahmawati Research Center for Vaccine and Drug, Research Organization for Health, National Research and Innovation Agency (BRIN), Cibinong, Indonesia
  • Fauzia Nurul Izzati Research Center for Vaccine and Drug, Research Organization for Health, National Research and Innovation Agency (BRIN), Cibinong, Indonesia
  • Rikno Harmoko Research Center for Genetic Engineering, National Research and Innovation Agency (BRIN), Bogor, 16911, West Java, Indonesia
  • Endang Saepudin Department of Chemistry, Faculty of Mathematics and Natural Science, Universitas Indonesia, Depok, 16424, Indonesia
  • Anis H. Mahsunah Research Center for Applied Microbiology, National Research and Innovation Agency (BRIN), Bogor, Indonesia

DOI:

https://doi.org/10.5614/j.eng.technol.sci.2022.54.4.13

Keywords:

aptamer, aptasensor, colorimetry, gold nanoparticles, penicillin G

Abstract

Antibiotics are chemical or biological substances that have the ability to kill pathogens selectively. Currently, high-performance liquid chromatography (HPLC) is used routinely in the detection of antibiotics. However, the cost of analysis and running time are bottlenecks for HPLC to be used for routine tests to detect antibiotics. Alternative methods need to be developed to overcome this issue. In this study, the development of a penicillin G specific biosensor by using a DNA aptamer and gold nanoparticles (AuNPs) was done. Optimal aptasensor conditions were achieved with the concentrations of NaCl and aptamer at 0.25 M and 2 ?M, respectively. An aptasensor of this type showed LOD for penicillin G at 3 mg/L and was able to detect penicillin G in the range of 3 to 27 mg/L. The established aptasensor showed specific sensitivity toward penicillin G after testing with several antibiotics, i.e., ampicillin, kanamycin, chloramphenicol, and erythromycin. The aptasensor could detect the presence of penicillin G from culture medium of wild-type, ultraviolet irradiation mutant, gamma irradiation mutant, and ultraviolet irradiation and gamma irradiation mutant strains of P. chrysogenum, at detection concentrations of 9.75 0.004; 25.25 0.005; 37.5 0.005; and 45 0.004 mg/L, respectively.

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Author Biography

Darmawati Darmawati, Department of Chemistry, Faculty of Mathematics and Natural Science, Universitas Indonesia, Depok, 16424, Indonesia

Aquaculture Study Program, Faculty of Fisheries, Universitas Madako Tolitoli,

Jalan Madako 1, Kelurahan Tambun, Tolitoli, 94515, Indonesia

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Published

2022-07-18

How to Cite

Darmawati, D., Mustopa, A. Z., Budiarto, B. R., Rahmawati, S. I., Izzati, F. N., Harmoko, R., Saepudin, E., & Mahsunah, A. H. (2022). Development of Gold Nanoparticle (AuNP)-based Colorimetric Aptasensor for Penicillin G Detection. Journal of Engineering and Technological Sciences, 54(4), 220413. https://doi.org/10.5614/j.eng.technol.sci.2022.54.4.13

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