Development of Single-Phase Microbial Cementation Method and to Investigate its Efficacy on Bearing Capacity, UCS, and Permeability of Sandy Soils

Authors

  • Prakash Bhaskarrao Kulkarni D Y Patil Institute of Engineering and Technology, Pune 410507 , Maharashtra,
  • Pravin Dinkar Nemade Vishwakarma Institute of Information Technology, Pune - 411048. Maharashtra,
  • Ranjit Chavan Municipal Corporation of Greater Mumbai, Mumbai C.S.T. 400001
  • Manoj Pandurang Wagh Vithalrao Vikhe Patil College of Engineering Ahmednagar, Maharashtra 414111

DOI:

https://doi.org/10.5614/j.eng.technol.sci.2021.53.6.2

Keywords:

bacterial culture and cementation (BCC) solution, microbial induced calcite precipitation (MICP), permeability, plate load test (PLT), ultimate bearing capacity (qu), unconfined compression strength (UCS)

Abstract

Microbially induced calcite precipitation (MICP) is a method based on collaborative knowledge of microbiology, chemistry and geotechnical engineering. The objective of this study was to investigate the increase of the bearing capacity and the unconfined compressive strength (UCS) as well as the reduction of the permeability of sandy soil using MICP. Experiments were carried out using Bacillus Pasteurii, on three different types of sand. The admixture of bacterial culture and cementation (BCC) solution all-in-one with sand by single-phase injection was applied to induce cementation. Three samples of the selected sand were treated with varied concentrations of BCC solution, ranging from 0.05 to 0.2 L/kg, with a curing period of 3, 7 and 14 days. The test results indicated an enhancement of 55% in UCS for sand treated with a BCC content of 0.05 to 0.2 L/Kg and a reduction of 40% in permeability for untreated sand with an effective diameter of 0.5 mm treated with 0.2 L/kg of BCC solution after 14 days of curing. The results of a plate load test (PLT) on MICP treated sand showed an increase in the ultimate bearing capacity (qu) by about 2.95 to 5.8 times and a 1.7 to 3.31-fold reduction in settlement corresponding to the same load applied on untreated footing. Further investigation of the size and shape of the bearing plate on bearing capacity and settlement was carried out through a plate load test. The higher and more favorable results shown by a rectangular plate compared to a circular plate indicate that the first is preferable.

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Author Biography

Prakash Bhaskarrao Kulkarni, D Y Patil Institute of Engineering and Technology, Pune 410507 , Maharashtra,

Vishwakarma Institute of Information Technology, Pune - 411048. Maharashtra, India

References

DeJong, J.T., Mortensen, B.M., Martinez, B.C. & Douglas, C.N., Bio-Mediated Soil Improvement, Ecological Engineering, 36(2), pp. 197-210, 2010.

DeJong, J.T., Michael, B., Fritzges, & Klaus, N., Microbially Induced Cementation to Control Sand Response to Undrained Shear, Journal of Geotechnical and Geo-Environmental Engineering, 132(11), pp. 1381- 1392, 2006. DOI: 10.1061/(ASCE)1090-0241(2006)132:11(1381).

Whiffin, V.S., Leon, A., van Paassen & Harkes, M.P., Microbial Carbonate Precipitation as a Soil Improvement Technique,Geomicrobiology Journal, 24(5), pp. 417-423, 2007.

Stabnikov, V., Maryam, N., Ivanov, V., Chu, J., Formation of Water-Impermeable Crust on Sand Surface Using Biocement, Cement and Concrete Research, 41(11), pp. 1143-1149, 2011.

Yasuhara, H., Hayashi, K. & Okamura, M., Evolution in Mechanical and Hydraulic Properties of Calcite-Cemented Sand Mediated by Biocatalyst, Geo-Frontiers, pp. 3984-3992, 2011. DOI 10.1061/41165(397)407.

Achal, V., Mukherjee, A. & Reddy, M.S., Microbial Concrete: Way to Enhance the Durability of Building Structures, Journal of Materials in Civil Engineering, 23(6), pp. 730-734, 2011. DOI 10.1061/(ASCE)MT.1943-5533.0000159

Montoya, B.M., DeJong, J.T. & Boulanger, R.W., Liquefaction Mitigation Using Microbial Induced Calcite Precipitation, State of the Art and Practice in Geotechnical Engineering, pp. 1918-1927, 2012. DOI 10.1061/9780784412121.197.

Chaerun, S.K., Rahayu, S., Rizki, N.I. & Pane, I., Utilization of a New Locally Isolated Bacterial Strain for Promoting Mechanical Properties of Mortar, Int J Civ Eng pp. 1-7, 2020. DOI: 10.1007/s40999-020-00500-z.

Li, L., Chun-xiang, Q. & Yong-hao, Z., Pore Structures and Mechanical Properties of Microbe-Inspired Cementing Sand Columns, Int J Civ Eng 12(2), pp. 174-179, 2014.

Nemati, M., Greene, E.A. & Voordouw, G., Permeability Profile Modification Using Bacterially Formed Calcium Carbonate: Comparison with Enzymic Option, Process Biochemistry, 40(2), pp. 925-933, 2005. DOI: 10.1016/j.procbio.2004.02.019.

Harkes, M.P., van Paassen, L.A. & Booster, J.L., Fixation and Distribution of Bacterial Activity in Sand to Induce Carbonate Precipitation for Ground Reinforcement, Ecological Engineering, 36(2), pp. 112-117, 2010. DOI 10.1016/j.ecoleng.2009.01.004.

Dejong, J.T. & Martinez, B., Upscaling of Bio-mediated Soil Improvement, Proceedings of the 17th International Conference on Soil Mechanics and Geotechnical Engineering, pp. 2300-2303, 2009.

Cheng, L. & Cord-Ruwisch, R., Upscaling Effects of Soil Improvement by Microbially Induced Calcite Precipitation by Surface Percolation, Geomicrobiology Journal, 131(5), pp. 396-406, 2014. DOI: 10.1080/ 01490451.2013.836579.

Deepika, K. & Wei-Ning Xiang, W., Review On Biologically Based Grout Material to Prevent Soil Liquefaction for Ground Improvement, International Journal of Geotechnical Engineering, 13(1), pp. 48-53, 2019. DOI: 10.1080/19386362.2017.1318478.

Dhami, N.K., Reddy, M.S. & Mukherjee, A., Biomineralization of Calcium Carbonates and Their Engineered Applications: A Review, Frontiers in Microbiology, 13(4), pp. 1-13, 2013. DOI: 10.3389/fmicb.2013.00314.

Anbu, P., Kang, C-H., Shin, Y-J. & So, J-S., Formations of Calcium Carbonate Minerals by Bacteria and Its Multiple Applications, Springer Plus, 5(1), pp. 1-26, 2016. DOI: 10.1186/s40064-016-1869-2.

Mobley, H.L. & Hausinger, R.P., Microbial Ureases: Significance, Regulation, and Molecular Characterization, Microbiol Rev, 53(1), pp. 85-108, 1989.

Mitchell, J.K. & Santamarina, J.C., Biological Considerations in Geotechnical Engineering, Journal of Geotechnical and Geoenvironmental Engineering, 131(10), pp. 1222-1233, 2005. DOI: 10.1061/(ASCE)1090-0241(2005)131:10(1222).

Al Qabany, A., Soga, K. & Santamarina, C., Factors Affecting Efficiency of Microbially Induced Calcite Precipitation, J. Geotech. Geoenviron. Eng., 138, pp. 992-1001, 2012. DOI: 10.1061/(ASCE)GT.1943-5606.0000666.

Nemati, M. & Voordouw, G., Modification of Porous Media Permeability, Using Calcium Carbonate Produced Enzymatically in Situ, Enzyme and Microbial Technology, 33(5), pp. 635-642, 2003. DOI: 10.1016/S0141-0229(03)00191-1.

Nemati, M., Greene, E.A. & Voordouw, G., Permeability Profile Modification Using Bacterially Formed Calcium Carbonate: Comparison with Enzymic Option, Process Biochemistry, 40, pp. 925-933, 2005. DOI: 10.1016/j.procbio.2004.02.019.

Wani, S.K. & Mir, B.A., Influence of Microbial Geo-Technology in The Stabilization of Dredged Soils, International Journal of Geotechnical Engineering, pp. 1-10, 2019. DOI: 10.1080/19386362.2019.1643099.

Martinez, B.C. & DeJong J.T., Bio-Mediated Soil Improvement: Load Transfer Mechanisms at the Micro- and Macro-scales, US-China Workshop on Ground Improvement Technologies, Advances in Ground Improvement, pp. 241-251, 2009. DOI 10.1061/41025(338)26.

Raghunandan, M., Juneja, A. & Hsiung, B., Preparation of Reconstituted Sand Samples in the Laboratory, International Journal of Geotechnical Engineering, 6(1), pp. 125-131, 2012. DOI: 10.3328/IJGE.2012.06.01. 125-131.

Hataf & Rahimi, Experimental Investigation of Bearing Capacity of Sand Reinforced with Randomly Distributed Tire Shreds, Construction and Building Materials 20, pp. 910-916, 2006. DOI: 10.1016/j.conbuildmat. 2005.06.019

Abu El-Soud & Bella, A.M., Bearing Capacity of Rigid Shallow Footing On Geogrid-Reinforced Fine Sand-Experimental Modeling, Arabian Journal of Geosciences, 11, 247, 2018. DOI: 10.1007/s12517-018-3597-0.

Fattah, M.Y., Shlash, K.T. & Mohammed, H.A., Experimental Study on the Behaviour of Strip Footing on Sandy Soil Bounded by a Wall, Arabian Journal of Geosciences, 8, 4779, 2015. DOI: 10.1007/s12517-014-1564-y.

Lin, H., Suleiman, M.T. & Jabbour, H.M., Enhancing The Axial Compression Response of Pervious Concrete Ground Improvement Piles Using Bio Grouting, Journal of Geotechnical and Geoenvironmental Engineering, 142(10), 04016045, 2016. DOI: 10.1061/(ASCE)GT. 1943-5606.0001515.

Suleiman, M.T., Lusu, N. & Raich, A., Development of Pervious Concrete Pile Ground-Improvement Alternative and Behaviour under Vertical Loading, Journal of Geotechnical and Geoenvironmental Engineering, 140(7), 04014035, 2014. DOI: 10.1061/(ASCE)GT. 1943-5606.0001135.

Cheng Liang, Shahin, M.A. & Chu, J., Soil Bio-Cementation Using a New One-Phase Low-pH Injection Method, Acta Geotechnica, 14(3), pp. 615-626, 2019. DOI: 10.1007/s11440-018-0738-2.

Mujah, D., Cheng, L. & Shahin, M.A., Microstructural and Geomechanical Study on Biocemented Sand for Optimization of MICP Process, J. Mater Civ. Eng., 31(4), pp. 1-10, 2019. DOI: 10.1061/(ASCE) MT.1943-5533.0002660.

Mahawish, A., Bouazza, A. & Gates, W.P., Improvement of Coarse Sand Engineering Properties by Microbially Induced Calcite Precipitation, Geomicrobiology Journal 35(10), pp. 887-897, 2018. DOI: 10.1080/ 01490451.2018.1488019.

Khaleghi, M. & Rowshanzamir, M.A., Biologic Improvement of a Sandy Soil Using Single and Mixed Cultures: A Comparison Study, Soil & Tillage Research, 186, pp. 112-119, 2019. DOI: 10.1016/j.still.2018.10.010.

Pakbaz, M.S., Behzadipour, H. & Ghezelbash, G.R., Evaluation of Shear Strength Parameters of Sandy Soils Upon Microbial Treatment, Geomicrobiology Journal, 35(8), pp. 721-726, 2018.

Changming, B., Kejun, W., Liu, S. & Ogbonnaya, U., Development of Bio-Cemented Constructional Materials Through Microbial Induced Calcite Precipitation, Materials and Structures, 51(1), pp. 30, 2018. DOI: 10.1617/ s11527-018-1157-4.

Chu, J., Stabnikov, V. & Ivanov, V., Microbially Induced Calcium Carbonate Precipitation on Surface or in the Bulk of Soil, Geomicrobiology Journal, 29(6), pp. 544-549, 2012. DOI: 10.1080/ 01490451.2011.592929.

Cheng, L., Cord-Ruwisch, R. & Shahin, M.A., Cementation of Sand Soil by Microbially Induced Calcite Precipitation at Various Degrees of Saturation, Canadian Geotechnical Journal, 50(1), pp. 81-90, 2013. DOI: 10.1139/cgj-2012-0023.

Nader, H. & Jamali, R., Effect of Fine-Grain Percent on Soil Strength Properties Improved by Biological Method, Geomicrobiology Journal, 35(8), pp. 695-703, 2018. doi 10.1080/01490451.2018.1454554.

Geonha, K., Lee, S. & Kim, Y., Subsurface Biobarrier Formation by Microorganism Injection for Contaminant Plume Control, Journal of Bioscience and Bioengineering, 101(2), pp. 142-148, 2006. DOI: 10.1263/ jbb.101.142.

Manav, P. & Manas, B., Effect of Different Shape of Footing on its Load-Settlement Behaviour (Circular, Square and Rectangular), Proceedings of the 4th World Congress on Civil, Structural, and Environmental Engineering (CSEE?19) Rome, Italy ? April, 2019. DOI: 10.11159/icgre19.168.

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Published

2021-12-17

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