Optimization of Laccase Production using White Rot Fungi and Agricultural Wastes in Solid-State Fermentation

Authors

  • Hendro Risdianto Center for Pulp and Paper, Ministry of Industry Jl. Raya Dayeuhkolot 132, Bandung, Jawa Barat 40258, Indonesia
  • Elis Sofianti Department of Chemical Engineering, Faculty of Industrial Technology, Institut Teknologi Bandung, Labtek X, Jl. Ganesa 10 Bandung, Jawa Barat 40132, Indonesia
  • Sri Harjati Suhardi School of Life Sciences and Technology, Institut Teknologi Bandung Labtek XI, Jl. Ganesha 10, Bandung, Jawa Barat 40132, Indonesia
  • Tjandra Setiadi Department of Chemical Engineering, Faculty of Industrial Technology, Institut Teknologi Bandung, Labtek X, Jl. Ganesa 10 Bandung, Jawa Barat 40132, Indonesia

DOI:

https://doi.org/10.5614/itbj.eng.sci.2012.44.2.1

Abstract

Abstract. Laccase has been produced in a solid-state fermentation (SSF) process using white rot fungi and various lignocellulose-based substrates. The white rot fungi used were Marasmius sp., Trametes hirsuta, Trametes versicolor and Phanerochaete crysosporium. The solid substrates employed in this research were collected from agricultural waste, specifically empty fruit bunches (EFB), rice straw, corncobs and rice husks. The objective of this research was to determine the most promising fungus, the best solid substrate and the optimal conditions for the production of laccase. The results showed that Marasmius sp. on all solid substrates displayed a higher laccase activity than that of any other strain of white rot fungi. Marasmius sp. and a solid substrate of rice straw demonstrated the highest laccase activity of 1116.11 U/L on day 10. Three significant factors, i.e. pH, temperature and yeast extract concentration, were studied by the response surface method on laccase production using Marasmius sp. and rice straw. The optimized conditions were a pH, temperature and yeast extract concentration of 4.9, 31C and 0.36 g/L, respectively. The fermentation of Marasmius sp. in SSF on agricultural waste shows a great potential for the production of laccase.

Downloads

Download data is not yet available.

References

Badan Pusat Statistik, Statistics Indonesia, http://dds.bps.go.id/tnmn_pgn.php?eng=1, (22 February 2011).

Hambali, E., Thahar, A., & Komarudin, A., The Potential of Oil Palm and Rice Biomass as Bioenergy Feedstock, in Proceeding of 7th Biomass Asia Workshop. Jakarta, Indonesia, 2010.

Couto, S.R. & Sanroman, M.A., Application of Solid-State Fermentation to Ligninolytic Enzyme Production. Biochemical Engineering Journal, 22, pp. 211-219, 2005.

Mitchell, D.A., Krieger, N. & BeroviA , M., Solid-State Fermentation Bioreactors: Fundamentals of Design and Operation, Berlin: Springer,2006.

Herrera, J.L.T., Osma, J.F. & Couto, S.R., Potential of Solid-State Fermentation for Laccase Production, Communicating Current Research and Educational Topics and Trends in Applied Microbiology, 2007.

Niladevi, K.N, Sukumaran, R.K. & Prema, P., Utilization of Rice Straw for Laccase Production by Streptomyces Psammoticus in Solid-State Fermentation, J. Ind. Microbiol. Biotechnol., 34, pp. 665-674, 2007.

Revankar, M.S., Desai, K.M. & Lele, S.S., Solid-State Fermentation for Enhanced Production of Laccase Using Indigenously Isolated Ganoderma sp., Appl. Biochem. Biotechnol., 143, pp. 16-26, 2007.

N1/4ske, J., Scheibner, K., Dornberger, U. & Hofrichter, M., Large Scale Production of Manganese-Peroxidase Using Whit-Rot Fungi, in Proceedings of the 8th International Conference on Biotechnology in the Pulp and Paper Industry, 4-8 June, Helsinki, Finland, 2001.

Gonzalez, G.V., Torres, E.F., Aguilar, C.N., Gomez, S.J.R., Godnez, G.D. & Augur, C., Advantages of Fungal Enzyme Production in Solid State Over Liquid Fermentation Systems, Biochemical Engineering Journal, 13, pp. 157-167, 2003.

Osma, J.F., Herrera, J.L.T. & Couto, S.R., Banana Skin: A Novel Waste for Laccase Production by Trametes Pubescens under Solid-State Conditions Application to Synthetic Dye Decolouration, Dyes and Pigments, 75, pp. 32-37, 2007.

Kunamneni, A., Ballesteros, A., Plou, F. J. & Alcalde, M., Fungal Laccase-A Versatile Enzyme for Biotechnological Applications, Communicating Current Research and Educational Topics and Trends in Applied Microbiology, pp. 233-245, 2007.

Krishna, C., Solid-State Fermentation Systems-An Overview, Critical Reviews in Biotechnology, 25, pp. 1-30, 2005.

Elisashvili, V., Kachlishvili, E. & Penninckx, M., Effect of Growth Substrate, Method of Fermentation, and Nitrogen Source on Lignocellulose-Degrading Enzymes Production by White-Rot Basidiomycetes, J. Ind. Microbiol. Biotechnol., 35, pp. 1531-1538, 2008.

Couto, S., Domnguez, A. & Sanroman, A., Utilisation of Lignocellulosic Waste for Lignin Peroxidase Production By Semi-Solid-State Culture of Phanerochaete Crysosporium, Biodegradation, 12, pp. 283-289, 2001.

Rosales, E., Couto, S.R. & Sanroman, A., New Uses of Food Waste: Application to Laccase Production by Trametes Hirsuta, Biotechnology Letters, 24, pp. 701-704, 2002.

Couto, S., Moldes, D., Liebanas, A. & Sanroman, M., Investigation of Several Bioreactor Configurations for Laccase Production by Trametes Versicolor Operating in Solid-State Conditions, Biochemical Engineering Journal, 15, pp. 21-26, 2003.

Bhmer, U., Kirsten, C., Bley, T. & Noack, M., White-Rot Fungi Combined with Lignite Granules and Lignitic Xylite to Decolorize Textile Industry Wastewater, Eng. Life Sci., 10, pp. 26-34, 2010.

Downloads

Issue

Section

Articles