Bacteria as Self-Healing Agent in Mortar Cracks

Ananto Nugroho; Iman Satyarno; Subyakto Subyakto

doi:10.5614/j.eng.technol.sci.2015.47.3.4

Authors

Ananto Nugroho Research Center for Biomaterials, Indonesian Institute of Sciences, Jalan Raya Bogor Km. 46, Cibinong, Bogor 16911,
Iman Satyarno Department of Civil and Environmental Engineering, Gadjah Mada University Jalan Grafika No. 2 Kampus UGM, Yogyakarta,
Subyakto Subyakto Research Center for Biomaterials, Indonesian Institute of Sciences, Jalan Raya Bogor Km. 46, Cibinong, Bogor 16911,

DOI:

https://doi.org/10.5614/j.eng.technol.sci.2015.47.3.4

Abstract

This study was aimed at finding the possibility to apply Bacillus subtilis integrated into mortar matrix to act as a self-healing agent to seal cracks. Bacterial spores at concentrations of 10⁴, 10⁵, and 10⁶ cells/ml were directly added into pulverized fly ash as medium to protect bacteria in high alkaline conditions. The results show that the addition of Bacillus subtilis spores into the mortar mixture enhanced the compressive strength, especially at a cell concentration of 10⁵ cells/ml. The bacterial mortar had a small ability to recover the stiffness of the mortar, amounting to 34.85% of its original stiffness. The effectiveness of crack sealant and resistance to water flow were limited to a maximum crack width size of 0.22 mm. Physical observation showed that the bacterial mortar is characterized by calcite precipitation as a product of ureolytic bacteria. The quantity and distribution of calcite precipitate depended on the precipitation weight, gravity direction and oxygen availability. Meanwhile, chemical analysis using XRD and EDX showed that the bacterial mortar had a better crystallinity.

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References

Tittelboom, K.V., Muynck, W.D., Belie, N.D. & Verstraete, W., Bacteria Protect and Heal Concrete and Stone, WTA Schriftenreihe, 33(2), pp. 439-457, Munich, Germany, 2009.

Ghosh, P., Mandal, S., Chattopadhyay, B.D. & Pal, S., Use of Microorganism to Improve the Strength of Cement Mortar, Cement and Concrete Research, 35(10), pp. 1980-1983, 2005.

Rodriguez, N.C., Rodriguez, G.M., Chekround, K.B. & Gonzalez, M.M.T., Conservation of Ornamental Stone by Myxococcus xanthus-induced Carbonate Biomineralization, Appl Environm Microbiol, 69(4), pp. 2182-2193, 2003.

Bang, S.S., Galinat, J.K. & Ramakrishnan, V., Calcite Precipitation Induced by Polyurethane-Immobilized Bacillus pasteurii, Enzyme and Microbial Technology, 28(4), pp. 404-409, 2001.

Ramachandran, S.K., Ramakrishnan, V. & Bang, S.S., Remediation of Concrete Using Micro-organisms, ACI. Materials, 98(1) pp. 3-9, 2001.

Jonkers, H.M. & Schlangen, E., Development of bacteria-based self-healing concrete, Tailor Made Concrete Structures, pp. 425-430, Amsterdam, the Netherlands, 2008.

Reddy, V.S., Rao, M.V.S., Aparna, P. & Sasikala, C., Performance of Standard Grade Bacterial (Bacillus subtilis) Concrete, Asian Journal of Civil Engineering, Building and Housing, 11(1), pp.43-55, 2010.

Chanal, N., Siddique, R. & Rajor, A., Influence of Bacteria on the Compressive Strength, Water Absorption and Rapid Chloride Permeability of Fly Ash Concrete, Construction and Building Material, 28(1), pp. 351-356, 2010.

Jonkers, H.M., Healing Agent Cement-Based Materials and Structures, and Process for its Preparation, Patent Application Publication, US 2011/0011303A1, 2011.

ASTM C-109/C109M, Standard Test Method for Compressive Strength of Hydraulic Cement Mortars (Using 2-in. or 50-mm Cube Specimens), West Conshohocken, Pennsylvania, USA, 2002.

ASTM C-348, Standard Test Method for Flexural Strength of Hydraulic-Cement Mortars, West Conshohocken, Pennsylvania, USA, 2002.

Kumar, B.G., Prabhakara P. & Pushpa H., Effect of Bacterial Calcite Precipitation on Compressive Strength of Mortar Cubes, International Journal of Engineering and Advanced Technology, 2(3), pp. 486-491, 2013.

Chandra, S., Waste Materials Used in Concrete Manufacturing, 1st ed., New Jersey, USA, Noyes Publications, 1997.