Adjusting Time of Flight in Ultrasound B-mode Imaging for Accurate Measurement of Fat using Image Segmentation Technique
DOI:
https://doi.org/10.5614/itbj.ict.res.appl.2017.11.1.1Keywords:
chicken fat, histogram mean, percentage of fat pixels, phase error, Soxhlet method, ultrasound B-mode.Abstract
This research attempted to measure chicken intramuscular fat content using improved ultrasound B-mode images and image segmentation. Adapted B-mode imaging is proposed to increase the positioning accuracy of B-mode images with the objective to correct the phase error due to the use of predetermined ultrasonic velocity in conventional B-mode imaging. The pre-determined velocity is replaced by actual velocity measured using A-mode imaging. The positioning accuracy of adapted and conventional B-mode imaging was validated using 144 chicken samples. The adapted B-mode image had better positioning accuracy compared to a conventional B-mode image since the method used was able to detect the thickness of the chicken sample with a lower mean difference (0.0360.034mm vs. 0.1130.010). Both methods were then applied for measurement of intramuscular fat content. The histogram mean and the percentage of fat pixels were the B-mode image characteristics that were extracted and their correlation with the fat content, measured using the Soxhlet method, was analyzed. The properties of the adapted B-mode images correlated better with the Soxhlet-measured fat content compared to the properties of the conventional B-mode images as reflected in the correlation coefficient, r, for the histogram mean (0.357 vs. 0.129) and the percentage of fat pixels (0.406 vs. 0.289). The results indicate the potential of using ultrasound adapted B-mode imaging to measure chicken intramuscular fat.Downloads
References
Miles, C., Shore, D. & Langley, K., Attenuation of Ultrasound in Milks and Creams, Ultrasonics, 28(6), pp. 394-400, 1990.
Javanaud, C., Gladwell, N., Gouldby, S. & Hibberd, D., Experimental and Theoretical Values of the Ultrasonic Properties of Dispersions: Effect of Particle State and Size Distribution, Ultrasonics, 29(4), pp. 331-337, 1991.
McClements, D., Povey, M. & Dickinson, E., Absorption and Velocity Dispersion due to Crystallization and Melting of Emulsion Droplets, Ultrasonics, 31(6), pp. 433-437, 1993.
McClements, D. & Povey, M., Ultrasonic Analysis of Edible Fats and Oils, Ultrasonics, 30(6), pp. 383-388, 1992.
Mason, T., Paniwnyk, L. & Lorimer, J., The Uses of Ultrasound in Food Technology, Ultrasonic Sonochemistry, 3(3), pp. S253-S260, 1996.
Abdul Halim, M.H., Ultrasound Velocity Measurement to Determine Fat Content in Chicken, Meat and Fish Fillet, Bachelor Degree Dissertation, Faculty of Electrical Engineering, Universiti Teknologi MARA, Malaysia, 2012.
Wild, J., The Use of Ultrasonic Pulses for the Measurement of Biologic Tissues and the Detection of Tissue Density Changes, Surgery, 27(2), pp. 183-188, 1950.
Hazel, L. & Kline, E., Ultrasonic Measurement of Fatness in Swine, J. Anim. Sci., 18(2), pp. 815-819, 1959.
Qu, X., Azuma, T., Liang, J. & Nakajima, Y., Average Sound Speed Estimation Using Speckle Analysis of Medical Ultrasound Data, Int. J. Comput. Assist Radiol Surg., 7(6), pp. 891-899, 2012.
Park, S., Lee, J., Lee, W. & Yoo, Y., Mean Sound Speed Estimation with Focusing Quality Evaluation for Medical Ultrasound Imaging, IEEE International Ultrasonics Symposium (IUS), Florida, United Statesm, pp. 2205-2208, 2011.
Krucker, J. & Fowlkes, J., Sound Speed Estimation using Automatic Ultrasound Image Registration, IEEE Transactions on Ultrasonics, Ferroelectrics, and Frequency Control, 51(9), pp. 1095-1106, 2004.
Abdul Halim, M.H., Buniyamin, N. & Shari, M., The Use of Ultrasound as a Fat Measurement Sensor for the Food Industry: A Review, 2013 IEEE International Conference on Smart Instrumentation, Measurement and Application (ICSIMA), Kuala Lumpur, Malaysia, 2013.
Ribeiro, F., Tedeschi, L. & Stouffer, J., Technical Note: A Novel Technique to Assess Internal Body Fat of Cattle by using Real-Time Ultrasound, Journal of Animal Science, 86(3), pp. 763-767, 2008.
Peres, A., Dias, L. & Joy, M., Assessment of Goat Fat Depots Using Ultrasound Technology and Multiple Multivariate Prediction Models, Journal of Animal Science, 88(2), pp. 572-580, 2010.
Bodwell, C. & Anderson, B., Nutritional Composition and Value of Meat and Meat Products, in Muscle as Food, P.J. Bechtel, P.J. Academic Press, Inc., Orlando, FL, pp. 321-369, 1986.
Halim, M. & Buniyamin, N., Improving Intramuscular Fat Measurement by Considering the Thickness of Protective Layer in Ultrasonic Transducer, 2014 International Conference on Electrical, Electronics, and System Engineering (ICEESE), pp. 101-107, 2014.
Worthy, G.A & Speakman, J.R., Body Composition Analysis of Animals: A Handbook of Non-Destructive Methods, Cambridge University Press, pp. 124-124, 2001.
Manirakiza, P., Covaci, A. & Schepens, P., Comparative Study on Total Lipid Determination using Soxhlet, Roese-Gottlieb, Bligh & Dyer, and Modified Bligh & Dyer Extraction Methods, Journal of Food Composition and Analysis, 14(1), pp. 93-100, 2001.
Allen, P. & McGeehin, B., Measuring the Lean Content of Carcasses Using TOBEC, The National Food Centre Research Report No. 40, Teagasc, Dublin, 2001.
Fiorotto, M., Cochran, W. & Funk, R., Total Body Electrical Conductivity Measurements: Effects of Body Composition and Geometry, American Journal of Physiology-Regulatory, Integrative and Comparative Physiology, 252(4), pp. R794-800, 1987.
Chanamai, R. & McClements, D., Ultrasonic Determination of Chicken Composition, Journal of Agricultural and Food Chemistry, 47(11), pp. 4686-4692, 1999.
Miles, C., Fursey, G., Fisher, A. & Page, S., Estimation of Lamb Carcass Composition from Measurements of the Speed of Ultrasound in the Soft Tissues of Live Animals and Carcasses, Meat Science, 30(3), pp. 245-256, 1991.
Houghton, P. & Turlington, L., Application of Ultrasound for Feeding and Finishing Animals: A Review, Journal of Animal Science, 70(3), pp. 930-941.1992.
Shannon, R., Probert-Smith, P. & Lines, J., Ultrasound Velocity Measurement to Determine Lipid Content in Salmon Muscle; the Effects of Myosepta, Food Research International, 37(6), pp. 611-620, 2004.
Whittaker, A., Park, B. & Thane, B., Principles of Ultrasound and Measurement of Intramuscular Fat, Journal of Animal Science, 70(3), pp. 942-952, 1992.
Ng, J., Rohling, R. & Lawrence, P., Automatic Measurement of Human Subcutaneous Fat with Ultrasound, IEEE Transactions on Ultrasonics, Ferroelectrics, and Frequency Control, 56(8), pp. 1642-1653, 2009.