The Effect of Acidity on the Growth and Chlorophyll a Content of Latoh (Caulerpa racemosa)

Authors

  • Caretta Trisari Vidyaningrum
  • Dicky Harwanto
  • Seto Windarto Universitas Diponegoro

DOI:

https://doi.org/10.5614/3bio.2026.8.1.3

Keywords:

Acidification, Chlorophyta, HCl, Macroalgae, Seagrapes

Abstract

Caulerpa racemosa is an edible green macroalga rich in chlorophyll. pH is a key environmental factor influencing seaweed performance, including growth and chlorophyll content. This study tested how pH affects growth and chlorophyll a in C. racemosa and identified the optimal pH. Experiments were conducted from November 2024 to January 2025 at the Center for Brackish Water Aquaculture (BBPBAP), Jepara, Central Java, using a completely randomised design with four treatments and three replicates: P0 (ambient pH), P1 (pH 8.25), P2 (pH 8.00), and P3 (pH 7.75). The highest biomass gain occurred at pH 8.25 (P1: 133.47 g), with the greatest specific growth rate also at pH 8.25 (P1: 3.02 % day-). The highest chlorophyll a content was observed under ambient pH (P0: 303.61 5.56 mg L-). pH significantly affected both growth and chlorophyll a of C. racemosa (ANOVA, P < 0.05). Water-quality variables (dissolved oxygen, temperature, salinity, light intensity, nitrate, and phosphate) remained within ranges suitable for C. racemosa throughout the study.

References

] Tapotubun AM, Theodora EAA, Matrutty, Jhohan R, Elizabeth JT, Eirene GF, Meigy NM, Welly AR, Beni S, Fredrik R. Seaweed Caulerpa sp position as functional food. MSFT [Internet]. 2020; 517,1-8. doi: 10.1088/1755- 1315/517/1/012021

] Windarto S, Kamaludin DM, Bella W, Sarjito, Pinandoyo, Susilowati T, Haditomo AHC, Harwanto D. Effect of coconut (Cocos nucifera) water, and aqueous extract of Mung bean (Vigna radiata) sprouts and Moringa (Moringa oleifera) leaf on the growth and nutrition of Caulerpa racemosa. International Journal of Aquatic Biology [Internet]. 2023; 11(6), 513?522. doi: 10.22034/ ijab.v11i6.2044

] Windarto S, Herawati VE, Wijaya YJ, Indriati DA, Rachmasari Y, Elfitasari T. Exploring the Physicochemical Properties and Nutritional Value of Abundant Seaweed Species Along the Jepara Coast, Indonesia. Thalassas [Internet]. 2025; 41, 82. Available from: doi: 10.1007/ s41208-025-00846-y

] Ciko? A, ?ubari? D, Roje M, Babi? J, Jerkovi? I, Joki? S. Recent advances on macroalgal pigments and their biological activities (2016?2021). Algal Research [Internet]. 2022; 65, 102748. doi: 10.1016/j. algal.2022.102748

] Muz-Miranda LA, Iguez-Moreno M. An extensive review of marine pigments: Sources, biotechnological applications, and sustainability. Aquatic Sciences [Internet]. 2023; 85(3), 68. doi: 10.1007/s00027-023-

-8

] Manzoor MF, Afraz MT, Y?lmaz BB, Adil M, Arshad N, Goksen G, Ali M, Zeng X. Recent progress in natural seaweed pigments: Green extraction, health- promoting activities, techno-functional properties and role in intelligent food packaging. Journal of Agriculture and Food Research [Internet]. 2024; 15, 100991. doi: 10.1016/j.jafr.2024.100991

] Rahman SM, Neaz S, Alam MM, Nur J. Hypolipidemic activity of ethanolic extract of Caulerpa racemosa. BIRDEM Medical Journal [Internet]. 2019; 9(3), 197-

doi: 10.3329/birdem.v9i3.43080

] Ebrahimi P, Shokramraji Z, Tavakkoli S, Mihaylova D,

Lante A. Chlorophylls as Natural Bioactive Compounds Existing in Food By-Products: A Critical Review. Plants [Internet]. 2023; 12(7), 1533. doi: 10.3390/ plants12071533

] Martins T, Barros AN, Rosa E, Antunes L. Enhancing Health Benefits through Chlorophylls and Chlorophyll- Rich Agro-Food: A Comprehensive Review. Molecules [Internet]. 2023; 28(14), 5344. doi: 10.3390/

molecules28145344

] IPCC. Climate Change 2014: Synthesis Report. Contribution of Working Groups I, II and III to the Fifth Assessment Report of the Intergovernmental Panel on Climate Change [CoreWriting Team, R. K. Pachauri and

L. A. Meyer (eds.)] 2014.151 pp.

] Gutow L, Mohammed MR, Kevin B, Reinhard S, Inka B, Christian W. Ocean acidification affects the growth but not nutritional quality of seaweed Fucus vesiculosus (Phaeophyceae, Fucales). Journal of Experimental Marine Biology and Ecology [Internet]. 2014; 453, 84-

doi: 10.1016/j.jembe.2014.01.005

] Kumar JGS, Umamaheswari S, Kavimani S dan Ilavarasan R. Pharmacological potential of green algae Caulerpa: A review. International Journal Pharmacy Science and Research [Internet]. 2019; 10 (3), 1014-

doi: 10.13040/IJPSR.0975-8232.10(3).1014-24

] Tito KC, Susilo E. Pengasaman Laut Di Perairan Indonesia. Journal of Fisheries and Marine Research [Internet]. 2021; 5(2), 419-426. doi: 10.21776/ ub.jfmr.2021.005.02.29

] Wanda P, Wibowo MA, Destiarti L. Enkapsulasi dan Uji Stabilitas Ekstrak Metanol Daun Pepaya (Carica papaya. Linn). JK [Internet]. 2017; 6(1), 25?29.

] Andika Y, Kawaroe M, Effendi H, Zamani NP. Pengaruh Kondisi pH Terhadap Respons Fisiologis Daun Lamun Jenis Cymodocea rotundata. Jurnal Ilmu dan Teknologi Kelautan Tropis [Internet]. 2020; 12(2), 487- 495. doi: 10.29244/jitkt.v12i2.21632

] Alda H, Ansar M, Arbitm NIS. Effect Different pH On Growth Performance Gracilaria changii. Jurnal Agribisnis [Internet]. 2022; 1(1), 41-50. doi: 10.33830/Agridev.v1i1.2891.2022

] Koca N, Karadeniz F, Burdurlu HS. Effect of pH on chlorophyll degradation and colour loss in blanched green peas. Food Chemistry [Internet]. 2006; 100(2), 609-615. doi: 10.1016/j.foodchem.2005.09.079

] Yilmaz C, Gokmen V. Chlorophyll. In Encyclopedia of Food and Health. Caballero B, Finglas PM, Toldra F. Waltham (US): Academic Press; 2016.

] Rifandi RA, Gunawan WS, Ali R. Pengaruh Konsentrasi Asam Klorida (HCl) Terhadap Mutu Alginat Rumput Laut Coklat Sargassum sp. Dari Perairan Teluk Awur Kab.Jepara Dan Poktunggal Kab. Gunungkidul. Journal Of Marine Research [Internet]. 2014; 3(4), 676-684. doi: 10.14710/jmr.v3i4.11430

] Lee WK, Lim YY, Ho CL. pH affects growth, physiology and agar properties of agarophyte Gracilaria changii (Rhodophyta) under low light intensity from Morib, Malaysia. Regional Studies in Marine Science [Internet]. 2019; 30, 100738. doi:10.1016/j.rsma.2019.100738

] Windarto S, Susilowati T, Haditomo AHC, Harwanto

D. Effect of exogenous natural plant growth regulators (PGRs) on the morphology, growth, and nutrient of sea grapes (Caulerpa racemosa). Aquaculture International [Internet]. 2024; 32, 3545?3562. doi: 10.1007/s10499-

-01337-8

] Harwanto D, Saputro P, Susilowati T, Haditomo AHC, Windarto S. Effect of different N:P ratios application on the cultivation media for the growth and fiber content of Calulerpa racemosa reared in tarpaulin ponds. AACL Bioflux [Internet]. 2020; 13(5) 3117 ? 3125.

] Windarto S, Prastiwahyudi AH, Susilowati T, Haditomo AHC, Harwanto D. Effect of Different Substrates on Growth and Protein Content of Caulerpa racemosa. Journal of Hunan University Natural Sciences [Internet]. 2021; 48(7): 266?275.

] Dini PSR, Susanto AB, Rini P. Pengaruh Konsentrasi Pupuk Cair Terhadap Pertumbuhan Dan Kandungan Klorofil-a Rumput Laut Gracilaria verrucosa (Harvey). Journal of Marine Research. 2021; 10(3): 327-332. doi: 10.14710/jmr.v10i3.29183

] Darson K, Yandhika SAG, Abd R. Citra Identifikasi Warna dengan Metode Nilai Hue Secara Realtime Berbasis Web Camera dan Raspberry Pi. Seminar Nasional Teknik Elektro, Sistem Informasi, dan Teknik Informatika [Internet]. 2023; 41-46. doi: 10.31284/p. snestik.2023.4100

] Utami M, Putra ED. Deteksi Objek Kualitas Daun Sawi Menggunakan Metode HSV Color dan Color Blob. JUSIBI (Jurnal Sistem Informasi Dan Bisnis) [Internet]. 2023; 5(2): 85?93.

] Pandiangan HSM. Segmentasi Citra Untuk Pencarian Kode Warna Cat Menggunakan Metode Thershold Hsv. Bulletin of Information Technology (BIT) [Internet]. 2020; 1(3), 134?143.

] Zonneveld N, Huisman EA, Boon JH. Prinsip-pinsip Budidaya Ikan. Jakarta: PT. Gramedia Pustaka Utama; 1991.

] Effendie MI. Metode Biologi Perikanan. Bogor: Yayasan Dewi Sri Bogor; 1979. 112p.

] Osorio C, Susana M, Juliana P, Silvia B, Filpa BP, Rita CA, Beatriz PM, Oliveira P. Pigments Content (Chlorophylls, Fucoxanthin and Phycobiliproteins) of Different Commercial Dried Algae. Separation [Internet]. 2013; 7(33): 1-14. doi: 10.3390/separations7020033

] Syad AN, Pandian S, Kasi PD. Seaweeds as nutritional supplements: Analysis of nutritional profile,

physicochemical properties and proximate composition of G. acerosa and S. wightii. Biomedicine & Preventive Nutrition [Internet]. 2013; (3):139?144. doi: 10.1016/j. bionut.2012.12.002

] Arnon DI. Copper enzymes in isolated chloroplasts: Polyphenoloxidase in Beta vulgaris. Plant Physiology [Internet]. 1949; 24(1), 1. doi: 10.1104/pp.24.1.1

] SNI 8567:2018. Penentuan kadar fosfat (PO 3-) pada air budidaya perikanan payau dan laut dengan metode spektrofotometri molibdat-asam askorbat.

] Stuthmann LE, Beatrice S, Springer K, Kunzmann A. Sea grapes (Caulerpa lentillifera J. Agardh, Chlorophyta) for human use: Structured review on recent research in cultivation, nutritional value, and post-harvest management. Journal of Applied Phycology [Internet]. 2023; 2957?2983. doi: 10.1007/s10811-023-03031-x

] Estrada JL, Bautista NS, Dionisio-sese ML. Morphological variation of two common sea grapes (Caulerpa lentillifera and Caulerpa racemosa) from selected regions in the Philippines. Biodiversitas Journal of Biological Diversity [Internet]. 2020; 21(5), 1823?

doi: 10.13057/biodiv/d210508

] Epifania VC, Eko S. Pencarian File Gambar Berdasarkan Dominasi Warna. Jurnal Buana Informatika [Internet]. 2011; 2(1),1-10. doi: 10.24002/jbi.v2i1.303

] Puspita D, Windu M, Arisia PSM. Pengaruh Konsentrasi Klorofil Krim Sup Caulerpa racemosa yang Dikeringkan Dengan Vacuum Drying Oven. Jurnal Teknologi Pangan dan Gizi [Internet]. 2021; 20(2), 94-101. doi: 10.33508/ jtpg.v20i2.3045/

] Susilowati A, Mulyawan AE, Yaqin K, Rahim SW. Kualitas air dan unsur hara pada pemeliharaan Caulerpa lentilifera dengan menggunakan pupuk kascing. Prosiding Seminar Nasional. 2017; 03, 275?282.

] Umam K, Arisandi A. Pertumbuhan Rumput Laut Eucheuma cottonii Pada Jarak Pantai yang Berbeda Di Desa Aengdake, Kabupaten Sumenep. Juvenil: Jurnal Ilmiah Kelautan dan Perikanan [Internet]. 2021; 2(2),115-124. doi: 10.21107/juvenil.v2i2.10672

] Nadlir A, Susilowati T, Adi K, Harwanto D, Haditomo AHC, Windarto S. Production Performance of Gracilaria verrucosa Using Verticulture Method with Various Wide Planting Area in Karimunjawa. Omni-Akuatika [Internet]. 2019; 15(1), 47-58. doi: 10.20884/1.oa.2019.15.1.671

] Darmawati, Rahmi JEA. Optimasi Pertumbuhan Caulerpa sp Yang Dibudidayakan Dengan Kedalaman Yang Berbeda di Perairan Laguruda Kabupaten Takalar. Octopus: Jurnal Ilmu Perikanan [Internet]. 2016; 5, 435?

] Taise A, Krieger E, Bury SJ, Christopher EC. Physiological responses of Caulerpa spp. (with different dissolved inorganic carbon physiologies) to ocean acidification. New Zealand Journal of Botany [Internet].

; 1-25. doi: 10.1080/0028825X.2023.2289432

] Damayanti T, Riris A, Fauziyah. Laju Pertumbuhan Rumput Laut Caulerpa racemosa Dengan Bobot Bibit Awal Berbeda Menggunakan Metode Rakit Apung dan Long Line Di PerairanTeluk Hurun, Lampung. Maspari Journal [Internet]. 2019; 11(1), 17 ? 22.

] Rendiansyah R, Arbit NIS, Saharuddin S. Pengaruh Pemberian Pupuk Urea Dengan Dosis Berbeda Terhadap Pertumbuhan Rumput Laut (Caulerpa lentillifera). Jurnal Teknologi Perikanan Dan Kelautan [Internet]. 2024; 15(1), 11-20. doi: 10.24319/jtpk.15.11-20

] Dimara L, Popy ILA, Efray W. Fotodegradasi, Uji pH dan Kandungan in Vivo Pigmen Klorofil-a Lamun Thalasia hemprichii. Jurnal Ilmu Kelautan dan Perikanan Papua [Internet]. 2018; 1(2), 76-83. doi: 10.31957/acr.v1i2.932

] Sasadara MMV, Ni Made DMWN, Putu ESK, Erna C, Ni Luh KAAD. Pengaruh Pemilihan Pelarut dalam Ekstraksi Klorofil pada Rumput Laut Gracilaria sp. dan Caulerpa sp. Segar dan Kering. Jurnal Ilmiah Medicamento [Internet]. 2023; 9(1), 22-28. doi: 10.36733/medicamento.v9i1.5344

] SNI 8554: 2018 Produksi Rumput Laut Lawi-Lawi (Caulerpa spp.) di Tambak.

] SNI 9049:2022 Bibit Rumput Laut Untuk Budidaya. [49.] Fauziah F. 2017. Pertumbuhan Sargassum Sp. Pada Tipe Habitat Dan Berat Koloni Berbeda Di Pantai Sakera Bintan. Skripsi. Universitas Maritim Raja Ali Haji. Tanjung Pinang.

] Pereira DT, Simioni C, Filipin EP, Bouvie F, Ramlov F, Maraschin M, Bouzon ZL, Schmidt C. Effects of salinity on the physiology of the red macroalga, Acanthophora spicifera (Rhodophyta, Ceramiales). Acta Botanica Brasilica [Internet]. 2017; 31 (4), 555-565. doi: 10.1590/0102-33062017abb0059

] Azizah MN, Rahman A, Balubi AM. Pengaruh Jarak Tanam Bibit yang Berbeda Terhadap Kandungan Agar Rumput Laut (Gracilaria verrucosa) Menggunakan Metode Longline di Tambak. Jurnal Media Akuatika [Internet]. 2018; 3(1), 556-563. doi: 10.33772/jma. v3i1.4379

Downloads

Published

2026-01-04