Comparison of Formulation Methods to Produce Nano-Chitosan as Inhibitor Agent for Bacterial Growth
DOI:
https://doi.org/10.5614/j.eng.technol.sci.2019.51.3.9Keywords:
anti-bacteria, ionic gelation, nano-chitosan, particle size, polyelectrolyte complex, precipitation, zeta potentialAbstract
Chitosan is known as an antibacterial agent. The effective surface area ratio of chitosan can be increased by modification into nanoparticles. Nano-chitosan can be prepared with several simple methods, i.e. precipitation, ionic gelation, or the polyelectrolyte complex method. This study compared these three methods in terms of the targeted product characteristics, i.e. stability of the average nanoparticle size as well as the colloidal dispersion, and the antibacterial characteristics. All three methods resulted in nanoparticle formation, but in the precipitation method significant zeta potential reduction was observed due to the presence of negative ions from the alkali that neutralized the chitosan amine group. The ionic gelation method yielded higher zeta potential and higher inhibition of bacterial growth than those yielded by the polyelectrolyte complex method. Ionic gelation and the polyelectrolyte complex method resulted in much better colloidal dispersion stability than the precipitation method, where a significant particle size increase was observed after one week of storage. This result indicates that both ionic gelation and the polyelectrolyte complex method can be used for forming nano-chitosan for the purpose of food preservation. However, for fishery products it is advisable to use the polyelectrolyte complex method because the TPP usually used in ionic gelation is not allowed to be applied to fish.
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References
Kean, T. & Thanou, M., Biodegradation, Biodistribution and Toxicity of Chitosan, Advanced Drug Delivery Reviews, 62(1), pp. 3-11, 2010.
Rodrigues, S., Dionsio, M., Lopez, C.R., Grenha, A., Biocompatibility of Chitosan Carriers with Application in Drug Delivery, Journal of Functional Biomaterials, 3(3), pp. 615-641, 2012.
Darwesh, O.M., Sultan, Y.Y., Seif, M.M. & Marrez, D.A., Bio-evaluation of Crustacean and Fungal Nano-chitosan for Applying as Food Ingredient, Toxicology Reports, 5, pp. 348-356, 2018.
Qi, L., Xu, Z., Jiang, X., Hu, C. & Zou, X., Preparation and Antibacterial Activity of Chitosan Nanoparticles, Carbohydrate Research, 339(16), pp. 2693-2700, 2004.
Aliasghari, A., Khorasgani, M.R., Vaezifar, S., Rahimi, F. & Khoroushi, M., Evaluation of Antibacterial Efficiency of Chitosan and Chitosan Nanoparticles on Cariogenic Streptococci: an In Vitro Study, Iran. J. Microbiol. 8(2), pp. 93-100, 2016.
Ramezani, Z., Zarei, M. & Raminnejad, N., Comparing the Effectiveness of Chitosan and Nanochitosan Coatings on the Quality of Refrigerated Silver Carp Fillets, Food Control, 51, pp. 43-48, 2015.
Wang, Y., Liu, L., Zhou, J., Ruan, X., Lin, J. & Fu, L., Effect of Chitosan Nanoparticle Coatings on the Quality Changes of Postharvest Whiteleg Shrimp, Litopenaeus vannamei, During Storage at 4 C, Food and Bio-process Technology, 8(4), pp. 907-915, 2015.
Abdou, E.S., Osheba, A.S. & Sorour, M.A., Effect of Chitosan and Chitosan-Nanoparticles as Active Coating on Microbiological Charac-teristics of Fish Fingers, Int. J. of Appl. Sci. and Tech., 2(7), p. 12, 2012.
Demir, G.M. & Degim, I.T., Preparation of Chitosan Nanoparticles by Nano Spray Drying Technology, FABAD J. Pharm. Sci., 38(3), pp. 127-133, 2013.
Chattopadhyay, D.P. & Inamdar, M.S, Studies on Synthesis, Charac-terization and Viscosity Behaviour of Nano Chitosan, Res. J. Engineering Sci., 1(4), pp. 9-15, Oct. 2012.
Agnihotri, S.A., Mallikarjuna, N.N., Aminabhavi, T.M., Recent Advances on Chitosan-based Micro- and Nanoparticles in Drug Delivery, Journal of Controlled Release, 100(1), pp. 5-28, Nov. 2004.
Pinho Neves, A.L. de, Milioli, C. C., M1/4ller, L., Riella, H.G., Kuhnen, N.C. & Stulzer, H.K., Factorial Design as tool in Chitosan Nanoparticles Development by Ionic Gelation Technique, Colloids and Surfaces A: Physicochemical and Engineering Aspects, 445, pp. 34-39, Mar. 2014.
Antoniou, J., Liu, F., Majeed, H., Qi, J., Yokoyama, W. & Zhong, F., Physicochemical and Morphological Properties of Size-controlled chitosan-tripolyphosphate Nanoparticles, Colloids and Surfaces A: Physicochemical and Engineering Aspects, 465, pp. 137-146, Jan. 2015.
Tan, C., Xie, J., Zhang, X., Cai, J. & Xia, S., Polysaccharide-based Nanoparticles by Chitosan and Gum Arabic Polyelectrolyte Complexation as Carriers for Curcumin, Food Hydrocolloids, 57, pp. 236-245, Jun. 2016.
Grenha, A., Chitosan Nanoparticles: A Survey of Preparation Methods, Journal of Drug Targeting, 20(4), pp. 291-300, 2012.
Hosseinnejad, M. & Jafari, S.M., Evaluation of Different Factors Affecting Antimicrobial Properties of Chitosan, International Journal of Biological Macromolecules, 85, pp. 467-475, 2016.
Sreekumar, S., Goycoolea, F.M., Moerschbacher, B.M. & Rivera-Rodriguez, G.R., Parameters Influencing the Size of Chitosan-TPP Nano- and Microparticles, Scientific Reports, 8(1), pp. 1-11, 2018.
Bhumkar, D.R. & Pokharkar, V.B., Studies on Effect of pH on Cross-Linking of Chitosan with Sodium Tripolyphosphate: A Technical Note, AAPS PharmSciTech, 7(2), pp. E138-E143, 2006.
Gonalves, A.A. & Ribeiro, J.L.D., Do Phosphates Improve the Seafood Quality? Reality and Legislation, Pan-American Journal of Aquatic Sciences, 3(3), pp. 237-247, 2008.
Pal, J., Shukla, N., Maurya, A.K., Verma, H.O., Pandey, G. & Amitha, A Review on Role of Fish in Human Nutrition with Special Emphasis to Essential Fatty Acid, International Journal of Fisheries and Aquatic Studies, 6(2), pp. 427-430, 2018.
Panzarasa, G., Osypova, A., Sicher, A., Bruinink, A. & Dufresne, R., Controlled Formation of Chitosan Particles by a Clock Reaction, Soft Matter, 14(31), pp. 6415-6418, 2018.
Primaningtyas, A., Budhijanto, W., Fahrurrozi, M. & Kusumastuti, Y., The Effects of Surfactant and Electrolyte Concentrations on the Size of Nanochitosan during Storage, AIP Conference Proceedings 1840, 080004, 2017.
Zhang, W. & Xia, W., Dissolution and Stability of Chitosan in a Sodium Hydroxide/urea Aqueous Solution, Journal of Applied Polymer Science, 131(3), Feb. 2014.
Mazancova, P., Nemethova, V., TreA3/4ova, D., KleaA kova, L., Lack, I. & Razga, F., Dissociation of Chitosan/tripolyphosphate Complexes into Separate Components upon pH Elevation, Carbohydrate Polymers, 192, pp. 104-110, Jul. 2018.
Coelho, S., Moreno-Flores, S., Toca-Herrera, J.L., Coelho, M.A.N., Pereira, M.C. & Rocha, S., Nanostructure of Polysaccharide Complexes, Journal of Colloid and Interface Science, 363(2), pp. 450-455, 2011.
Tsai, R.-Y., Kuo, T-Y., Hung, S-C., Lin, C-M., Hsien, T-Y., Wang, D-M. & Hsie, H-J., Use of Gum Arabic to Improve the Fabrication of Chitosan-gelatin-based Nanofibers for Tissue Engineering, Carbohydrate Polymers, 115, pp. 525-532, 2015.
Morris, G.A., Castile, J., Smith, A.G., Adams, G. & Harding, S.E., The Effect of Prolonged Storage at Different Temperatures on the Particle Size Distribution of Tripolyphosphate (TPP)-chitosan Nanoparticles, Carbohydrate Polymers, 84(4), pp. 1430-1434, 2011.
Salopek, B., Krasi, D. & Filipovi, S., Measurement and Application of Zeta-Potential, Rudarsko-geoloSko-naftni Zbornik, 4, pp. 147-151, 1992.
Gumustas, M., Sengel-Turk, C.T., Gumustas, A., Ozkan, S.A. & Uslu, B., Effect of Polymer-Based Nanoparticles on the Assay of Antimicrobial Drug Delivery Systems, in Multifunctional Systems for Combined Delivery, Biosensing and Diagnostics, Elsevier, pp. 67-108, 2017.
Gierszewska-DruA1/4yA,ska, M., Ostrowska-Czubenko, J. & Kwiatkowska, A., Effect of Ionic Crosslinking on Density of Hydrogel Chitosan Membranes, Progress on Chemistry and Application of Chitin and its Derivatives, 18, pp. 49-58, 2013.
Espinosa-Andrews, H., Baez-Gonzalez, J.G., Cruz-Sosa, F. & Vernon-Carter, E.J., Gum Arabic-Chitosan Complex Coacervation, Biomacro-molecules, 8(4), pp. 1313-1318, 2007.
Gonalves, N.D., Grosso, C.R.F., Rabelo, R.S., Hubinger, M.D. & Prata, A.S., Comparison of Microparticles Produced with Combinations of Gelatin, Chitosan and Gum Arabic, Carbohydrate Polymers, 196, pp. 427-432, 2018.
Holappa, J., Hjalmarsdottir, M., Masson, M., Runarsson, -., Asplund, T., Soininen, P., Nevalainen, T. & Jarvinen, T., Antimicrobial Activity of Chitosan N-betainates, Carbohydrate Polymers, 65(1), pp. 114-118, Jul. 2006.
Ibekwe, C.A., Oyatogun, G.M., Esan, T.A. & Oluwasegun, K.M., Synthesis and Characterization of Chitosan/Gum Arabic Nanoparticles for Bone Regeneration, American Journal of Materials Science and Engineering, 5(1), pp. 28-36, Sep. 2017.