Comparative Study of Bacterial Cellulose Film Dried Using Microwave and Air Convection Heating


  • I. Indriyati Research Unit for Clean Technology, Indonesian Institute of Sciences, Jalan Sangkuriang Komplek LIPI Gedung 50, Bandung, Indonesia 2Research Center for Physics, Indonesian Institute of Sciences, Kawasan Puspiptek Serpong, Tangerang Selatan,
  • Yuyun Irmawati Research Center for Physics, Indonesian Institute of Sciences, Kawasan Puspiptek Serpong, Tangerang Selatan,
  • Tita Puspitasari Center for Application of Isotope and Radiation, National Nuclear Agency of Indonesia, Jalan Lebak Bulus Raya No. 49, Jakarta



bacterial cellulose, convection oven, edible film, microwave, swelling, vacuum filtration


An investigation was conducted to analyze and compare the properties of bacterial cellulose (BC) films dried using microwave and air convection heating. Prior to the drying process, 90% of the water content inside the BC pellicles was removed through vacuum filtration. After that, the required drying time was only 3-5 min using microwave heating, 95% shorter than that observed for air convection heating. The properties of the BC sheets were characterized by Fourier transform infrared (FTIR), X-ray diffractometer (XRD), color difference meter, and tensile tester machine. The results showed that the structure of the BC films was the same for the BC films dried by microwave and air convection heating, i.e. cellulose I, as observed from FTIR spectra and XRD diagrams. Moreover, their color (L*, a*, and b* values) and mechanical properties were also almost identical. However, a slightly lower crystallinity and a higher swelling degree were observed for the BC film dried using microwave heating. These results suggest that microwave heating could be an alternative method of drying BC pellicles in order to shorten the processing time and reduce energy consumption when compared to air convection heating.


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Saibuatong, O. & Philasapong, M., Novo Aloe Vera-Bacterial Cellulose Composite Film from Biosynthesis, Carbohydrate Polymers, 79, pp. 455-460, 2010.

Wan, Y.Z., Hong, L., Jia, S.R., Huang, Y., Zhu, Y., Wang, Y.L. & Jiang, H.J., Synthesis and Characterization of Hydroxyapatite - Bacterial Cellulose Nanocomposites, Compost Science and Technology, 66, pp. 1825-1832, 2006.

Czaja, W.K., Young, D.J., Kawecki, M. & Brown, R.M., The Future Prospects of Microbial Cellulose in Biomedical Applications, Biomacromolecules, 8, pp. 1-12, 2006.

Svensson, A., Nicklasson, E., Harrah, T., Panilaitis, B., Kaplan, D.L., Brittberg, M. & Gatenholm, P., Bacterial Cellulose as A Potential Scaffold for Tissue Engineering of Cartilage. Biomaterials, 26(4), pp. 419-431, 2005.

Klemm, D., Schumann, D., Udhardt, U. & Marsch, S., Bacterial Synthesized Cellulose - Artificial Blood Vessels for Microsurgery, Progress in Polymer Science, 26(9), 1561-1603, 2001.

Nishi, Y., Uryu, M., Yamanaka, S., Watanabe, K., Kitamura, N., Iguchi, M., & Mitsuhashi, S., The Structure and Mechanical Properties of Sheets Prepared from Bacterial Cellulose, Part 2 Improvement of The Mechanical Properties of Sheets and Their Applicability to Diaphragms of Electroacoustic Transducers, Journal of Materials Science, 25(6), 2997-3001, 1990.

Indrarti, L. & Indriyati, Incorporation of Citrus Essential Oils into Bacterial Cellulose-Based Edible Films and Assessment of Their Physical Properties, IOP Conference Series: Earth and Environmental Science, 60, p. 012018, 2017.

Zeng, M., Laromaine, A. & Roig, A., Bacterial Cellulose Films: Influence of Bacterial Strain and Drying Route on Film Properties, Cellulose, 21, pp. 4455-4469, 2014.

Pa'e, N., Abd Hamid, N.I., Khairuddi, N., Zahan, K.A., Seng, K.F., Siddique, B.M. & Muhamad, I.I., Effect of Different Drying Methods on The Morphology, Crystallinity, Swelling Ability and Tensile Properties of Nata De Coco, Sains Malaysiana, 43(5), pp. 767-773, 2014.

Zhang, C.J., Wang, L., Zhao, J.C. & Zhu, P., Effect of Drying Methods on Structure and Mechanical Properties of Bacterial Cellulose Films, Advanced Materials Research, 239-242, pp. 2667-2670, 2011.

Kaya, S. & Kaya, A., Microwave Drying Effects on Properties of Whey Protein Isolate Edible Films, Journal of Food Engineering, 43, pp. 91-96, 2000.

Cardenas, G., Daz, V.J., Melendrez, M.F. & Cruzat, C.C., Physicochemical Properties of Edible Films from Chitosan Composites Obtained by Microwave Heating, Polymer Bulletin, 61, pp. 737-748, 2008.

Hou, A., Wang, X., & Wu, L., Effect of Microwave Irradiation on The Physical Properties and Morphological Structures of Cotton Cellulose, Carbohydrate Polymers, 74, pp. 934-937, 2008.

Rao, P.P., Nagender, A., Rao, L.J. & Rao, D.G., Studies on The Effects of Microwave Drying and Cabinet Tray Drying on the Chemical Composition of Volatile Oils of Garlic Powders, European Food Research and Technology, 224, pp. 791-795, 2007.

Kouchakzadeh, A. & Shafeei, S., Modeling of Microwave Convective Drying of Pistachios, Energy Convertion and Management, 51, pp. 2012-2015, 2010.

Embuscado, M.E., Marks, J.S. & BeMiller, J.N., Bacterial Cellulose. I. Factors Affecting the Production of Cellulose by Acetobacter Xylinum, Food Hydrocolloids, 8, pp. 407-418, 1994.

Indriyati, Yudianti, R. & Indrarti, L., Development of Bacterial Cellulose/Activated Carbon Composites Prepared by in Situ and Cast-drying Methods, Asian Transaction on Basic and Applied Sciences, 02, pp. 21-24, 2012.

Segal, L., Creely, J.J., Martin Jr., A.E. & Conrad, C.M., An Empirical Method for Estimating the Degree of Crystallinity of Native Cellulose Using the X-Ray Diffractometer, Textile Research Journal, 29(10), pp. 786-794, 1959.

Wong, R.S.H., Ashton, M. & Dodou, K., Investigation of Crosslinking Agent Concentration on the Properties of Unmedicated Hydrogels, Pharmaceutics, 7, pp. 305-319, 2015.

Lidstrom, P., Tierney, J., Wathey, B. & Westman, J., Microwave Assisted Organic Synthesis: A Review, Tetrahedron, 57, pp. 9225-9283, 2001.

Zhang, M., Tang, J., Mujumdar, A.S. & Wang, S., Trends in Microwave-Related Drying of Fruits and Vegetables, Trends in Food Science and Technology, 17(10), pp. 524-534, 2006.

Hazervazifeh, A., Moghaddam, P.A. & Nikbakht, A.M., Microwave Dehydration of Apple Fruit: Investigation of Drying Efficiency and Energy Cost, Journal of Food Process Engineering, 40(3), p. e12463, 2016.

Ul-Islam, M., Shah, N., Ha, J.H. & Park, J.K., Effect of Chitosan Penetration on Physic-Chemical and Mechanical Properties of Bacterial Cellulose, Korean Journal of Chemical Engineering, 28, pp. 1736-1743, 2011.

Halib, N., Mohd Amin, M.C.I. & Ahmad, I., Physicochemical Properties and Characterization of Nata De Coco from Local Food Industries as A Source of Cellulose, Sains Malaysiana, 41(2), pp. 205-211, 2012.

Sugiyama, J., Persson, J. & Chanzy, H., Combined Infrared and Electron Diffraction Study of the Polymorphism of Native Celluloses, Macromolecules, 24, pp. 4168-4175, 1991.

Ul-Islam, M., Khan, T. & Park, J.K., Nanoreinforced Bacterial Cellulose-Montmorillonite Composites for Biomedical Applications, Carbohydrate Polymers, 89(4), pp. 1189-1197, 2012.

Czaja, W., Romanovicz, D. & Brown, R.M., Structural Investigations of Microbial Cellulose Produced in Stationary and Agitated Culture, Cellulose, 11, pp. 403-411, 2004.

Wada, M. & Okano, T., Localization of I and I Phases in Algal Cellulose Revealed by Acid Treatments, Cellulose, 8, pp. 183-188, 2001.

Santos, S.M., Carbajo, J.M., Quintana, E., Ibarra, D., Gomez, N., Ladero, M., Eugenio, M.E. & Villar, J.C., Characterization of Purified Bacterial Cellulose Focused on Its Use on Paper Restoration, Carbohydrate Polymers, 116, pp. 173-181, 2015.

Phisalaphong, M., Suwanmajo, T. & Sangtherapitikul, P., Novel Nanoporous Membranes from Regenerated Bacterial Cellulose, Journal of Applied Polymer Science, 107, pp. 292-299, 2008.

Clasen, C., Sultanova, B., Wilhelms, T., Heisig, P. & Kulicke, W-M., Effects of Different Drying Processes on the Material Properties of Bacterial Cellulose Membranes, Macromolecular Symposia, 244, pp. 48-58, 2006.

Mayachiew, P. & Devahastin, S., Comparative Evaluation of Physical Properties of Edible Chitosan Films Prepared by Different Drying Methods, Drying Technology: An International Journal, 26(2), pp. 176-185, 2008.

Gea, S., Reynol, C.T., Roohpour, N., Wirjosentono, B., Soykeabkaew, N., Billoti, E. & Peijs, T., Investigation into The Structural, Morphological, Mechanical and Thermal Behaviour of Bacterial Cellulose after A Two-Step Purification Process, Bioresource Technology, 102(19), pp. 9105-9110, 2011.