Ficus elastica Leaf Extract Effect Toward IL-10 and TNF-? as a Preeclampsia Model on Hypoxia-induced EA.hy926


  • Chrismis Novalinda Ginting Faculty of Medicine, Universitas Prima Indonesia, Jalan Belanga No. 1, Medan, 20118, North Sumatera, Indonesia
  • I Nyoman Ehrich Lister Faculty of Medicine, Universitas Prima Indonesia, Jalan Belanga No. 1, Medan, 20118, North Sumatera, Indonesia
  • Ermi Girsang Faculty of Medicine, Universitas Prima Indonesia, Jalan Belanga No. 1, Medan, 20118, North Sumatera, Indonesia
  • Maya Sari Mutia Faculty of Medicine, Universitas Prima Indonesia, Jalan Belanga No. 1, Medan, 20118, North Sumatera, Indonesia
  • Yolanda Eliza Putri Lubis Faculty of Medicine, Universitas Prima Indonesia, Jalan Belanga No. 1, Medan, 20118, North Sumatera, Indonesia
  • Annisa Amalia Biomolecular and Biomedical Research Center, Aretha Medika Utama, Jalan Babakan Jeruk II No. 9, Bandung, 40163, West Java, Indonesia
  • Wahyu Setia Widodo Biomolecular and Biomedical Research Center, Aretha Medika Utama, Jalan Babakan Jeruk II No. 9, Bandung, 40163, West Java, Indonesia
  • Dwi Surya Artie Biomolecular and Biomedical Research Center, Aretha Medika Utama, Jalan Babakan Jeruk II No. 9, Bandung, 40163, West Java, Indonesia
  • Rizal Rizal Biomedical Engineering, Department of Electrical Engineering, Faculty of Engineering, Universitas Indonesia, Jalan Kampus UI, Depok, 16424, West Java, Indonesia
  • Wahyu Widowati Faculty of Medicine, Maranatha Christian University, Jalan Surya Sumantri No. 65, Bandung, 40164, West Java Indonesia



EA.hy926, Ficus elastica, hypoxia, IL-10, preeclampsia, TNF-?


Preeclampsia is a pregnancy complication characterized by proteinuria, hypertension, hypoxia as well an increased systemic inflammatory response. It affects 5 to 10% of all births and is a leading factor in fetal and maternal morbidity and death. Ficus elastica L. leaves are known to have natural compounds with anti-inflammatory and antioxidant properties. This research assessed the potential of F. elastica leaf extract (FEE) as anti-inflammatory agent in a hypoxic-induced human endothelial cell line (EA.hy926) as preeclampsia model. F. elastica leaves were extracted using 70% ethanol. Cytotoxic assay of the FEE in different concentrations (3.13 to 100 g/ml) against EA.hy926 was done using MTS assay. The FEE?s anti-inflammatory activity was measured with ELISA for the TNF-? and IL-10 parameters. The FEE did not cause cytotoxicity to the EA.hy926 cells, with viability above 80% at the highest concentration. The FEE could decrease the pro-inflammatory TNF-? level and increase the IL-10 level as anti-inflammatory markers in the preeclampsia model at significance level p<0.05. This study suggests that FEE is a safe alternative herbal medicine for preeclampsia treatment.


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