The Potency of Trigona’s Propolis Extract as Reactive Oxygen Species Inhibitor in Diabetic Mice
Keywords:alloxan, diabetes mellitus, hyperglycemia, oxidative stress, Trigona’s propolis, reactive oxygen species (ROS).
AbstractHyperglycemia has been proven to increase oxidative stress due to the production of reactive oxygen species (ROS) that exceed the capabilities of the natural antioxidant defenses, causing a deficiency in insulin receptors and insulin resistance. In this study, the effect of propolis on ROS was observed. Fifty five (55) male mice (Mus musculus SW.) were divided into 5 groups, i.e. KN (normal control), KDM (diabetes control), and P1, P2, P3. Propolis solution 50, 100 and 175 mg/kg bw was given to groups P1, P2 and P3 respectively, while distilled water was given to groups KN and KDM by oral gavage for 21 days. Density of ROS was measured every 7 days, while measurement of plasma insulin was carried out every 3 days. The results show that the density of ROS in the groups treated with propolis was lower than in the KDM group. However, the plasma insulin levels in the propolis groups were higher than in the KDM group. It was concluded that propolis can decrease ROS density and causes an increase in plasma insulin levels.
Rohilla, A. & Ali, S., Alloxan Induced Diabetes: Mechanism and Effects, International Journal of Research in Pharmaceutical and Biomedical Science, 3(2), pp. 819-822, 2012.
Shaw, J.E., Saltiel, A.R. & Zimmet, P.Z., Global Estimates of the Prevalence of 2010 and 2030, Diabetes Research and Clinical Practice, 87(1), pp. 4-14, 2010.
Shulman, G.I., Cellular Mechanism of Insulin Resistance, The Journal of Clinical Investigation, 106(1), pp. 19-26, 2000.
Can, A., Nuriye, Ozov, N., Bolkent, S. & Yanardag, R., Effect of Aloe Vera Leaf and Pulp Extracts on the Liver in Type II Diabetic Rat Models, Biological and Pharmaceutical Bulletin, 27(5), pp. 694-698, 2004.
Evan, J.L., Goldfine, I.D., Maddux, B.A. & Grodsky, G.M., Oxidative Stress-Activated Signaling Pathway Mediators of Insulin Resistance and -cells Dysfunctions?, Diabetes, 52(1), pp. 1-8, 2003.
Robertson, R.P., Harmon, J., Tran, P.O., Tanaka, Y. & Takahashi, H., Glucose Toxycity in Beta-Cells, Type 2 Diabetes, Good Radical Gone Bad, and the Gluthation Connection, Diabetes, 52(3), pp. 581-587, 2003.
Jee, S.H., Kim, H.J. & Lee, J., Obesity Insulin Resistance and Cancer Risk, Yonsei Medical Journal, 46(4), pp. 449-455, 2005.
Okutan, H., Ozcelik N., Yilmaz, H.R. & Uz, E., Effect of Caffeic Acid Phenethyl Ester on Lipid Peroxidation and Antioxidant Enzymes in Diabetic Rat Heart, Clinical Biochemistry, 38(2), pp. 192-196, 2005.
Kaneto, H., Fuji, J., Myint, T., Miyazawa, N., Islam, K.N., Kawasaki, Y., Suzuki, K., Nakamura, M., Tatsumi, H., Yamasaki, Y. & Taniguchi, N., Reducing Sugars Trigger Oxidative Modification and Apoptosis in Pancreatic -Cells by Provoking Oxidative Stress Through the Glycation Reaction, Biochemical Journal, 320(3) pp. 855-863, 1996.
Kaneto, H., Kajimoto, Y., Miyagawa, J. & Hori, M., Beneficial Effects of Antioxidants in Diabetes: Possible Protection of Pancreatic -cells againts Glucose Toxicity, Diabetes, 48(12), pp. 2398-2406, 1999.
Houstis, N., Rosen, E.D. & Lander, E.S., Reactive Oxygen Species Have a Causal Role in Multiple Forms of Insulin Resistance, Nature, 440(7), pp. 944-948, 2006.
Bonnard. C., Durand. A., Peyrol. S., Chanseaume. E., Chauvin M.A., Mario. B., Vidal. H. & Rieusset. J., Mitochondrial Dysfunction Results from Oxidative Stress in the Skeletal Muscle of Diet-Induced Insulin-Resistance Mice, Journal of Clinical Investigation, 118(2), pp. 789-800, 2008.
Kumashiro, N., Tamura, Y., Uchida, T., Ogihara, T., Fujitani, Y., Hirose, T., Mochizuki, H., Kawamori, H. & Watada, H., Impact of Oxidative Stress and Peroxixome Proliferator-Activated Receptor " Coactivator-1 in Hepatic Insulin Resistance, Diabetes, 57(8) pp. 2083-2091, 2008.
James, D.E., The Role of ROS in Insulin Resistance. Australian Physiological Society, 41, pp. 187-189, 2010.
Tabrani, M.M., Level of Plasma Insulin and Blood Sugar after Administration of Neem (Azadirachta Indica A. Juss) Extract to Diabetic Male Mice (Mus Musculus L.). Magister Biology Thesis, SITH-ITB, 2007 (Text in Indonesian).
Riany, H., Effect of Polyphenol to Expression Insulin- Receptor at Muscle of Diabetic Mice. Magister Biology Thesis, SITH-ITB, 2011. (Text in Indonesian)
Ridwan, A., Astrian, R.T. & Barlian, A. Measurement of Antidiabetic Polyphenol (Polyphenol 60) based on Blood Sugar Levels and Pancreatic Histology of Diabetic Male Mice (Mus Musculus L.). Jurnal Matematika dan Sains. 17(2), pp. 78- 82, 2012. (Text in Indonesian)
Burdock, G.A., Review of the Biological Properties and Toxicity of Bee Propolis, Food and Chemical Toxicology, 36(4), pp. 347-363, 1998.
Radiati, L.E., Thohari, I. & Agustina, N.H., Study of Propolis, Pollen, and Royal Jelly of Honey Products as Natural Antioxidant, Jurnal Ilmu dan Teknologi Hasil Ternak, 2(1), pp. 35-39, 2006. (Text in Indonesian)
Benguedouar, L., Boussenane, H.N., Wided, K., Alyane, M., Rouibah, H. & Lahouel, M., Efficiency of Propolis Extract Against Mitochondrial Stress Induced by Antineoplasic Agents (Doxorubicin And Vinblastin) in Rats, Indian Journal Exploration Biology, 46(2), pp. 112-119, 2008.
Margeretha, I., Study of Bioactive Ingredient of Trigona spp. as Anticaries Agent through Bioassay Guided Chemical Analysis Approach. Doctoral Degree Dissertation. Fakultas Kedokteran Gigi, Universitas Indonesia, 2012. (Text in Indonesian)
Sosnowski, Z.M., Method for Extracting Propolis and Water Soluble Dry Propolis Powder, US Patent 4382886 A, 1983.
Dikalov, A., Griendling, K.K. & Harrison, D.G., Measurement of Reactive Oxygen Species in Cardiovascular Studies, Hypertension, 49, pp. 717-727, 2007.
Szkudelski, T., The Mechanism of Alloxan and Streptozotocin Action -Cells of the Rat Pancreas. Physiology, 50, pp. 536-546, 2001.
Mani, F., Damasceno, H.C., Novelli, E.L., Martins, E.A. & Sforcina, J.M., Propolis: Effect of Different Concentrations, Extracts and Intake Period. Journal Enthopharmacology, 105(2), pp. 95-98, 2006.
Nijveldt, R.J., van Nood, E.E., van Hoorn, D.E., Boelens, P.G. & van Leeuwen, P.A., Flavonoids: A Review of Probable Mechanisms of Action and Potential Applications, Diabetes, 74(4), pp. 418-425, 2001.
Sari, A.N. Effect of Propolis on Reactive Oxygen Species (ROS) Density in Diabetic Mice, Magister Biology Thesis, SITH-ITB, 2013. (Text in Indonesian)