The Potency of Camellia Sinensis L. to Reduce Proinflammatory Cytokine Levels in the Acute Respiratory Distress Syndrome Rat Model
DOI:
https://doi.org/10.5614/j.math.fund.sci.2023.55.1.6Keywords:
acute respiratory distress syndrome, Camellia sinensis, COVID-19, inflammation, lungAbstract
This study was conducted in order to ascertain how green tea extract (GTE) could affect inflammatory markers, including level of interleukin-(IL)-12, IL-18 of serum and lung, tumor necrosis factor (TNF)-?, gene expression of NLR family-pyrin-domain containing 3 (NLRP3) of lung, nuclear factor kappa B (NF-?B), lung histopathology, and IL-6 expression of lung tissue in lipopolysaccharide (LPS)-treated rats as ARDS animal model. Rats were given GTE at dosages of 0, 50, 400, 800 mg/kg of body weight for 28 days to boost their immune systems. The rats were then stimulated with LPS (5 g/kg of BW) and after that continued to receive GTE for 28 days. Levels of serum or lung IL-18, IL-12, TNF-?, were measured using the ELISA method; expression of lung NF-?B and NLRP3 was measured by qRT-PCR; immunohistochemistry (IHC) was implemented to assess lung IL-6 expression; and lung histopathology was evaluated through the bleeding, inflammation, and alveolus scores. GTE had the ability to lower serum IL-18, lung TNF-?, and lung IL-12 levels; suppress the lung gene expression of NF-?B, NLRP-3, IL-6 expression; and improve lung histopathology. Green tea extract inhibited inflammation in the ARDS rat model by decreasing the proinflammatory cytokine level and proinflammatory gene expression.
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