Active Fractions of Black Rice Bran cv Cempo Ireng Inducing Apoptosis and S-phase Cell Cycle Arrest in T47D Breast Cancer Cells


  • Rarastoeti Pratiwi Laboratory of Biochemistry, Faculty of Biology, Universitas Gadjah Mada, Jalan Teknika Selatan, Sekip Utara,Yogyakarta, 55281, Indonesia
  • Alfi Rizqi Amalia Postgraduate student, Faculty of Biology, Universitas Gadjah Mada, Jalan Teknika Selatan, Sekip Utara, Yogyakarta, 55281
  • Woro Anindito Sri Tunjung Laboratory of Biochemistry, Faculty of Biology, Universitas Gadjah Mada, Jalan Teknika Selatan, Sekip Utara,Yogyakarta, 55281
  • Rumiyati Rumiyati Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Gadjah Mada, Sekip Utara, Yogyakarta, 55281



apoptosis, cell cycle, Cempo Ireng, cytotoxicity, S-phase


Indonesian black rice cv Cempo Ireng was evaluated for cancer prevention using a T47D cell line model. Methanolic extract of black rice bran (BRB) showed cytotoxicity with an IC50 of 522.13 g/ml. This result was in contrast to water extract of BRB, which yielded an IC50 of more than 6000 g/ml. From the methanolic extract of BRB, its 6 fractions were found from the preparative TLC performance, with Rf values ranging from 0 to 0.97. According to the MTT assay of all fractions, fraction 3 (F3) had the lowest IC50 (60.17 1.72g/ml), while fraction 2 (F2) had an IC50 of 64.3 0.61g/ml. However, F2 showed the highest induction of apoptosis, i.e. about 75.39 0.43% compared to the T47D cells without treatment (control) (about 8.39 0.16%) and with doxorubicin treatment (about 41.30 0.08%). Furthermore, fraction 5 (F5) not only induced apoptosis but also increased the S-phase arrest percentage (60.36 2.07%), which was significantly higher than for the other treatments. Therefore, each fraction of BRB showed different cytotoxic responses to T47D cells.


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