Bioremediation of Phenanthrene, Chrysene, and Benzo[a]pyrene by Fungi Screened from Nature

Authors

  • Tony Hadibrata 1Department of Applied Bioscience, Faculty of Agriculture, Ehime University. 3-5-7 Tarumi, Matsuyama, Ehime, 790-8566, Japan. 2Laboratory of Pulp and Paper, Faculty of Forestry, Mulawarman University. Kampus Gunung Kelua, Samarinda 75119, Indonesia.
  • Sanro Tachibana 1Department of Applied Bioscience, Faculty of Agriculture, Ehime University. 3-5-7 Tarumi, Matsuyama, Ehime, 790-8566, Japan.

DOI:

https://doi.org/10.5614/itbj.sci.2009.41.2.3

Abstract

Laccase of Polyporus sp. S133 was able to oxidize most of the 3 different rings amount polycyclic aromatic hydrocarbons (PAHs) tested. Phenanthrene was removed by 89% followed by chrysene and benzo[a]pyrene which were oxidized by 66 and 55%, respectively. Addition of 1-hydroxybenzotriazole (HBT) to the reaction mixture increased oxidation of PAHs, especially phenanthrene was almost completely removed from the reaction mixture. Oxidation of chrysene and benzo[a]anthracene increased 12 and 10% with the mediator to 78 and 65% in the presence of HBT. PAH-quinones as oxidation products were formed from all PAH to different extents. A part of PAH was polymerized in the laccase/mediator system to products of weight-average molecular weight (MW). The correlation of the ionization potentials of PAH with the oxidation of these compounds is limited to the alternating PAH.

References

Mueller, J.G., Lantz, S.E., Blattmann B.O. & Chapman, P.J., Bench-Scale Evaluation of Alternative Biological Treatment Process for The Remediation of Pentachlorophenol and Creosote Contaminated Materials: Solid Phase Bioremediation, Environ. Sci. Technol., 25, 1045-1055, 1991.

Kastner, M. & Mahro, B., Microbial Degradation of Polycyclic Aromatic Hydrocarbons (PAHs) in Soils Affected by The organic Matrix Of Compost, Appl. Microbiol. Biotechnol., 44, 668-675, 1996.

Park, K.S., Sims, R.C. & Dupont, R., Transformation of PAHs in Soil Systems. J. Environ. Eng., 116, 632-640, 1990.

Cerniglia, C.E., Biodegradation of Polycyclic Aromatic Hydrocarbon, Biodegradation, 3, 351-368, 1992.

Couto R.S., Herrera, J.T., Industrial Biotechnological Applications of Laccases: A Review, Biotechnol. Adv., 24, 500-513, 2006.

Baldrian P., Purification and Characterization of Laccase from The White-Rot Fungus Daedalea Quercina and Decolorization of Synthetic Dyes by The Enzyme, Appl. Microbiol. Biotechnol., 63, 560-563, 2004.

Thurston C.F., The Structure and Function of Fungal Laccases, Microbiol., 140, 19-26, 1994.

Lowry, O.H., Rosebrough, N.J, Farr, A.L. & Randall, R.J., Protein Measurement with The Folin Phenol Reagent, J. Biol. Chem., 193, 265-275, 1951.

Hatvani, N. & Mecs, I., Production of Laccase and Manganese Peroxidase by Lentinus Edodes on Malt Containing by Product of the Brewing Process, Process Biochem., 37, 491-496, 2001.

Laessoe, T., Lincoff, G., Edwards, E.H. & Fletcher, N., Mushrooms, Dorling Kindersley Publishers Ltd., London, 2002.

Pace, G., Mushrooms of the World: With 20 Photographs and 634 Full Color Illustrations of Species and Varieties, Firefly Books Ltd., New York, 1998.

Johannes, C., Majcherczyk, A. & Huttermann, A., Degradation of Anthracene by Laccase of Trametes Versicolor in the Presence of Different Mediator Compounds, Appl. Microbiol. Biotechnol., 46, 313-317, 1996.

Collins, P.J., Kotterman, M.J.J., Field, J.A. & Dobson, A.D.W., Oxidation of Anthracene and Benzo[A]Pyrene by Laccases from Trametes Versicolor, Appl. Environ. Microbiol., 62, 4563-4567, 1996.

Kawai, S., Nakagawa, M. & Ohashi, H., Aromatic Ring Cleavage of A Non-Phenolic O-4 Lignin Model Dimer by Laccase of Trametes Versicolor in The Presence of 1-Hydroxybenzotriazole, FEBS Lett., 446, 355-358, 1999.

Tominaga, J., Michizoe, J., Kamiya, N., Ichinose, H., Maruyama, T. & Goto, M., Factors Affecting The Oxidative Activity of Laccase Towards Biphenyl Derivatives in Homogeneous Aqueous-Organic Systems, J. Biosci. Bioeng., 98, 14-19, 2004.

Fabbrini, M., Galli, C. & Gentili, P., Comparing The Catalytic Efficiency of Some Mediators of Laccase, J. Mol. Catal. B: Enzym., 16, 231-240, 2002.

Gibson, D.T. & Subramanian, V., Microbial Degradation of Aromatic Hydrocarbons, In: Gibson DT, editor. Microbial degradation of organic compounds. Marcel Dekker Inc., New York, pp. 181-252, 1984.

Cerniglia, C.E. & Yang, S.K., Stereoselective Metabolism of Anthracene and Phenanthrene by The Fungus Cuninghamella Elegans, Appl. Environ. Microbiol., 47, 119-24, 1984.

Cerniglia, C.E., Biodegradation of Polycyclic Aromatic Hydrocarbons, Biodegradation, 3, 351-368, 1992.

Downloads

Issue

Vol. 41 No. 2 (2009)

Section

Articles