Bioremediation of Phenanthrene, Chrysene, and Benzo[a]pyrene by Fungi Screened from Nature


  • Tony Hadibrata 1Department of Applied Bioscience, Faculty of Agriculture, Ehime University. 3-5-7 Tarumi, Matsuyama, Ehime, 790-8566, Japan. 2Laboratory of Pulp and Paper, Faculty of Forestry, Mulawarman University. Kampus Gunung Kelua, Samarinda 75119, Indonesia.
  • Sanro Tachibana 1Department of Applied Bioscience, Faculty of Agriculture, Ehime University. 3-5-7 Tarumi, Matsuyama, Ehime, 790-8566, Japan.



Laccase of Polyporus sp. S133 was able to oxidize most of the 3 different rings amount polycyclic aromatic hydrocarbons (PAHs) tested. Phenanthrene was removed by 89% followed by chrysene and benzo[a]pyrene which were oxidized by 66 and 55%, respectively. Addition of 1-hydroxybenzotriazole (HBT) to the reaction mixture increased oxidation of PAHs, especially phenanthrene was almost completely removed from the reaction mixture. Oxidation of chrysene and benzo[a]anthracene increased 12 and 10% with the mediator to 78 and 65% in the presence of HBT. PAH-quinones as oxidation products were formed from all PAH to different extents. A part of PAH was polymerized in the laccase/mediator system to products of weight-average molecular weight (MW). The correlation of the ionization potentials of PAH with the oxidation of these compounds is limited to the alternating PAH.


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