Flavonoid Compounds from the Herb of Krokot (Lygodium microphyllum) and their antioxidant activity against DPPH


  • Hadi Kuncoro Faculty of Pharmacy, Mulawarwan University, Samarinda, 75119, Kalimantan Timur
  • Kindi Farabi Faculty of Pharmacy, Mulawarwan University, Samarinda, 75119, Kalimantan Timur
  • Laode Rijai Faculty of Pharmacy, Mulawarwan University, Samarinda, 75119, Kalimantan Timur
  • Euis Julaeha Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Padjadjaran, Jatinangor 45363, Sumedang
  • Unang Supratman Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Padjadjaran, Jatinangor 45363, Sumedang
  • Yoshihito Shiono Department of Bioresources Engineering, Faculty of Agriculture, Yamagata University, Tsuruoka-shi, Yamagata 997-8555




Antioxidant, DPPH, flavonoids, Lygodium microphyllum, Lygodiaceae


Six flavonoid compounds, kaempferol (1), quercetin (2), acacetin (3), quercetin-3-O-β-D-glucopyranoside (4), kaempferol-3-O-β-D-glucopyranoside (5), and isorhamnetin-3-O-β-D-glucopyranoside (6), were isolated from the methanol extract of the Lygodium microphyllum herb. The chemical structures of compounds 1-6 were identified on the basis of spectroscopic evidence and comparison with previously reported spectral data. Compounds 1-6 were isolated for first time from this plant. Their antioxidant activity against DPPH (2,2-Diphenyl-1-picrylhydrazyl) was evaluated. Among the compounds, quercetin (2) showed high antioxidant activity with IC50 values of 6.94 0.03 μg/mL.


Zeng-Fu, L., Huil, H., Hang-Yi, Z. & Jun-Chen, Z., Review on the Extraction of Flavonoids from Fern, Journal of Sanming University, 25(2), pp. 200-203, 2008.

Guo-Gang, Z., Ying-Cui, H., Hong-Xia, L., Lin-Xia, Z. & Li-Juan, C., The Research of Lygodium, Intechopen, Shanghai, 2012.

Zheng, X.L. & Xing, F.W., Ethnobotanical Study on Medicinal Plants around Mt. Yinggeling, Hainan Island, China, Journal of Ethnopharmacology, 124(2), pp. 197-210, 2009.

Zhang, L., Yin, Z. & Ye, W., Flavonoids from Lygodium Japonicum, Biochemical Systematics and Ecology, 34, pp. 885-886, 2006.

Ye, W., Fan, C., Zhang, L., Yin, Z. & Zhao, S., A New Phenolic Glycoside from the Roots of Lygodium Japonicum, Fitoterapia, 78(7-8), pp. 600-601, 2007.

Chen, L., Zhang, G., He, J., Jin Guan, J., Pan, C., Mi, W. & Wang, Q., New Naphthoquinone from the Root of Lygodium Japonicum (Thunb.) Sw. Journal of Natural Medicine, 64, pp. 114-116, 2010.

Zhu, L., Zhang, G., Chen, L., Wang, S., Li, P. & Li, L., A New Ecdysteroside from Lygodium japonicum (Thunb.) Sw, Journal of Natural Medicinal, 63, pp. 215-219, 2009.

Katja, D.G., Sonda, A.A., Harneti, D.P., Mayanti, T. & Supratman, U., 7-hydroxy-6-metoxy cumarin (schopoletin) from the Bark of Chisocheton celebicus (Meliaceae), Jurnal Kimia Indonesia, 1, pp. 10-14, 2017. (Text in Indonesian)

Duan, Y., Dai, He, Y.R., Kurihara, H., Li, Y. & Yao, X., A New Phenylpropanoid Glucoside from the Aerial Parts of Lygodium Japonicum, Journal of Asian Natural Products Research, 14(3), pp. 286-292, 2012.

Lei, J., Xiao, Y., Huang, J., Tang, M. & Deng, P., Two New Kaempferol Glycosides from Androsace Umbellata, Helvetica Chimica Acta, 92(7), pp. 1439-1444, 2009.

Chaturvedula, V.S.R. & Prakash, I., Isolation of Stigmasterol and -sitosterol from the Dichloromethane Extract of Rubus Suavissimus, International Current Pharmaceutical Journal, 1(9), pp. 239-242, 2012.

Mouffok, S., Haba, H., Lavaud, C., Long, C. & Benkhaled, M., Chemical Constituents of Centaurea omphalotricha Coss. & Durieu ex Batt. & Trab, Records of Natural Products, 6(3), pp. 292-295, 2012.

Gomes, R.A., Ramirez, R.R.A., Maciel, J.K.S., Agra, M.F., Souza, M.F.V. & Falcao-silva, V.S., Phenolic Compounds from Sidastrum micranthum (A. St.-Hil.) fryxell and Evaluation of Acacetin and 7,4'-Di-O-methylisoscutellarein as Modulator of Bacterial Drug Resistence, Quimica Nova, 34(8), pp. 283-286, 2011.

Islam, M., Al-Amin, M.D., Siddiqi, M.M.A., Akter, S., Haque, M.M., Sultana, N. & Chowdhury, A.S., Isolation of Quercetin-3-O-beta-D-Glucopyranoside from the Leaves of Azadirachta Indica and Antimicrobial and Cytotoxic Screening of the Crude Extracts, Dhaka University Journal of Science, 60(1), pp. 11-14, 2012.

Sianturi, J., Farabi, K., Mayanti, T., Harneti, D., Darwati, Supratman, U., Awang, K. & Hayashi, H., Antioxidant Constituents from the Bark of Aglaia eximia (Meliaceae), Makara Journal of Science, 20(1), pp. 1-6, 2016.

Liu, H., Mou, Y., Zhao, J., Wang, J., Zhou, L., Wang, M., Wang, D., Han, J., Yu, Z. & Yang, F., Flavonoids from Halostachys caspica and their Antimicrobial Antioxidant Activities, Molecules, 15(11), pp. 7933-7945, 2010.