Induction of Somatic Embryos from Leaf and Stem Nodal Section Explants of Potato (Solanum tuberosum L.)


  • I. Iriawati School of Life Sciences & Technology Institut Teknologi Bandung
  • Fitri Aprilianty Biology Department of School of Life Sciences and Technology, Institut Teknologi Bandung Jl. Ganesha 10 Bandung 40132, Indonesia



Somatic embryogenesis can be used as an alternative method to obtain high-quality potato seedlings. This research was conducted in order to evaluate: the potential of different types of explants to produce somatic mbryos;combinations of 2,4-D and BAP for somatic embryo induction; and the ability of BAP to act as growth regulator for the somatic embryo maturation process.Young leaf and stem nodal sections were cultured in MS media supplemented with 2,4-D and BAP. The developing calli were then transferred into media containing several concentrations of 2,4-D and BAP. The embryogenic calli were then transferred into media containing 1-5 μM BAP. The results show that MS media containing 2,5 μM 2,4-D + 5 μM BAP and μM 2,4-D + 0.5 μM BAP were the most suitable for inducing somatic embryos from leaf explants and stem nodal section explants, respectively. The somatic embryos were well-developed in the MS media supplemented with 5 μM BAP. The stem nodal section explants were able to produce a higher percentage of globular, heart and torpedo stage embryos compared with the leaf explants. Based on this research, stem nodal section explants have a high potential for producing somatic embryos induced in medium containing 1 μM 2,4-D + 0.5 μM BAP.


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