Induction of Somatic Embryos from Leaf and Stem Nodal Section Explants of Potato (Solanum tuberosum L.)
DOI:
https://doi.org/10.5614/j.math.fund.sci.2014.46.3.8Abstract
Somatic embryogenesis can be used as an alternative method to obtain high-quality potato seedlings. This research was conducted in order to evaluate: the potential of different types of explants to produce somatic mbryos;combinations of 2,4-D and BAP for somatic embryo induction; and the ability of BAP to act as growth regulator for the somatic embryo maturation process.Young leaf and stem nodal sections were cultured in MS media supplemented with 2,4-D and BAP. The developing calli were then transferred into media containing several concentrations of 2,4-D and BAP. The embryogenic calli were then transferred into media containing 1-5 μM BAP. The results show that MS media containing 2,5 μM 2,4-D + 5 μM BAP and μM 2,4-D + 0.5 μM BAP were the most suitable for inducing somatic embryos from leaf explants and stem nodal section explants, respectively. The somatic embryos were well-developed in the MS media supplemented with 5 μM BAP. The stem nodal section explants were able to produce a higher percentage of globular, heart and torpedo stage embryos compared with the leaf explants. Based on this research, stem nodal section explants have a high potential for producing somatic embryos induced in medium containing 1 μM 2,4-D + 0.5 μM BAP.References
Wattimena, G.A., Propagule Development of Qualified and Superior Potato Cultivar for Improving Potato Production in Indonesia, Full-time Professorship Scientific paper in Horticultural Science, Faculty of Agriculture, Bogor Agriculture Institute, 2000. (Text in Indonesian)
Kuntjoro, A.S., Virus Free G0 Potato Minituber Production through Plantlet Micropropagation in PT. Intidaya Agrolestari (Inagro) Bogor-West Java, Theses, Agricultural Cultivation, Faculty of Agriculture, Bogor Agriculture Institute, 2000. (Text in Indonesian).
Purnamaningsih, P., Plant Regeneration through Somatic Embryogenesis and Its Controlling, Buletin Agrobiologi, 5(2), pp. 51-58, 2002. (Text in Indonesian).
JayaSree, T., Pavan, U., Ramesh, M., Rao, A.V., Jagan Mohan Reddy, K. & Sadanandam, A., Somatic Embryogenesis from Leaf Cultures of Potato, Plant Cell, Tissue and Organ Culture, 64, pp. 13-17, 2001
Newman, P.G., Krishnaraj, S. & Saxena, P.K., Regeneration of Tomato (Lycopersicon esculentum Mill): Somatic Embryogenesis and Shoot Organogenesis from Hypocotyl Explants Induced with 6-benzyladenine, International Journal of Plant Sciences, 157, pp. 554-560, 1996.
Dudits, D., Gyorgyey, J., Bogre, L. & Bako, L. Molecular Biology of Somatic Embryogenesis, in Thorpe T.A. (ed.), In vitro Embryogenesis in Plants, Kluwer Academic Publishers, Dordrecht, Boston, London, pp. 267-308, 1995.
Krishna, R.S. & Vasil, I.K., Somatic Embryogenesis in Herbaceous Monocots, in Thorpe, T.A. (ed.), In Vitro Embryogenesis in Plants, Kluwer, Dordrecht, pp. 417-470, 1995.
Neumann, K-H., Kumar, A. & Imani, J., Plant Cell and Tissue Culture - a Tool in Biotechnology, Principles and Practice, Springer-Verlag, Berlin, Heidelberg, pp. 96, 2009.
Litz, R.R. & Gray, D.J., Somatic Embryogenesis for Agricultural Improvement, World Journal of Microbiology and Biotechnology, 11, pp. 416-425, 1995.
Murashige, T. & Skoog, F., A Revised Medium for Rapid Growth and Bioassays with Tobacco Tissue Cultures, Physiol. Plant, 15, pp. 473-497, 1962.
Purnamaningsih, R., Callus Induction and Optimization of Regeneration in Four Rice Varieties through In Vitro Culture, J. Agronomi & Biogenetik, 2, pp. 74-80, 2006. (Text in Indonesian)
Sellars, R.M., Southward, G.M. & Philips, G.C., Adventitious Somatic Embryogenesis from Culture Immature zygotic Embryos of Peanut and Soybean, Crop Science, 30, pp. 408-413, 1990.
Maillot, P., Kieffer, F. & Walter, B., Somatic Embryogenesis from Stem Nodal Sections of Grapevine, Vitis, 45, pp. 185-189, 2006.
George, E.F., Hall, M.A. & De Klerk, G-J., Plant Propagation by Tissue Culture, 3rd Ed., Springer, Dordrecht, p. 177, 2008.
Kiyosue, S., Satoh, S., Kamada, H. & Harada, H., Somatic Embryogenesis in Higher Plants, Journal Plant Research Spec. Issue, 3, pp. 75-82, 1993.
Philips, G.C., Hubstenberger, J.F. & Hansen, E.E., Plant Regeneration from Callus and cell suspension Cultures by Somatic Embryogenesis, Plant Cell, Tissue and Organ Culture, Springer Lab. Manual, pp. 81-101. 1995.
Priyono & Danimihardja, S., Somatic Embryo Reproduction of Coffee Arabica by Using BAP and Kinetin, and Plantlet Regeneration by Using Ca-P and Biotin, in Proceedings of Seminar in Plantation Biotechnology and Industrial Biopolymer Workshop, Inter University Center, Bogor Agriculture Institute, 1991. (Text in Indonesian)
