Evaluation of Batch Culture Phytoremediation Process using Local Hydromacrophytes to Reduce Synthetic Pesticide Residue in Contaminated Irrigation Water


  • Catur Retnaningdyah Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Brawijaya, Jalan Veteran, Malang 65145
  • Endang Arisoesilaningsih Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Brawijaya, Jalan Veteran, Malang 65145




diatom biotic indices, evaluation, irrigation water, phytoremediation, Prevathon


The aim of this study was to evaluate the effectivity of batch culture phytoremediation to remediate irrigation water that was contaminated with 6.4 L/L synthetic pesticide Prevathon. This was a true experimental research study using a completely randomized design, conducted in a 30 L bucket with sand and gravel as the substrate. The treatment comprised five types of hydromacrophytes (emergent, floating leaf, submerged, polyculture and control without plants), repeated three times at the same time. The effectiveness of the phytoremediation model was determined by water physicochemical parameters, periphyton diversity, percentage of pollution tolerant values (%PTV) and trophic diatom index (TDI) on day 6, 13, 29 and 37 after incubation. The research results showed significantly decreased values of biochemical oxygen demand, turbidity, bicarbonate, nitrate, orthophosphate, total phosphate and free chlorine after incubation for 6 days; decreased concentration of chemical oxygen demand after incubation for 13 days. The water quality improved from slightly and moderately polluted (diversity index 1.6-2.8) to clean and slightly polluted (diversity index 2.8-4.2), from moderately and heavily organically polluted (PTV 40.0-93.7%) to slightly organically polluted and free of organic pollution (PTV 2.4-34.1%), and from meso-eutrophic and eutrophic (TDI 37.4-70.4) to oligo and meso-eutrophic (TDI 13.7-26.4).


JICA, The Study for Formulation Development Program in the Republic of Indonesia, Final Report, Volume I. Executive Summary, November 1995.

Wimbaningrum, R., Indriyani, S., Retnaningdyah, C. & Arisoesilaningsih, E., Monitoring Water Quality Using Biotic Indices of Benthic Macroinvertebrates along Surfaces Water Ecosystems in Some Tourism Areas in East Java, Indonesia, J. Ind. Tour. Dev. Std., 4(2), pp. 81-90, 2016.

Habiebah, R.A.S. & Retnaningdyah, C., Evaluation of Water Quality due to Human Activities in the Sumber Awan Spring and Its Chanel, Singosari Malang, 2(1), pp. 40-45, 2014. (Text in Indonesia)

Kartikasari, D., Retnaningdyah, C. & Arisoesilaningsih, E., Application of Water Quality and Ecology Indices of Benthic Macroinvertebrate to Evaluate Water Quality of Tertiary Irrigation in Malang District, J.Trop. Life Science, 3(3), pp. 193-201, 2013.

Retnaningdyah, C. & Arisoesilaningsih E., Water Sources Pollution Indication in the Watershed of Malang Raya Brantas using Biotic Index of Macroinvertebrate Bentos, Prosiding Seminar Nasional Biodiversitas, Ridwan, M., Pamungkas, D.W., Kharismamurti, K., Widiyanti, N., Putri, R.D.A., Noviana, Y., (Eds), 3(1), pp. 210-213, 2014. (Text in Indonesia)

Daam, M.A. & Brink, P.J.V., Implications of Differences Between Temperate and Tropical Freshwater Ecosystems for The Ecological Risk Assessment of Pesticides, Ecotoxicol., 19, pp. 24-37, 2010.

Keller, A.E., Acute Toxicity of Several Pesticides, Organic Compounds, and A Wastewater Effluent to the Freshwater Mussel, Anodontai Mbecilis Ceriodaphnia Dubia, and Pimephalesp Romelas, Environ. Contain. Toxicol., 51, pp. 696-702, 1993.

Damasio J, Navarro-Ortega, A., Tauler, R., Lacorte, S., Barcelo, D., Soares, A.M.V.M., Lopez, M.A., Riva, M.C. & Barata, C., Identifying Major Pesticides Affecting Bivalve Species Exposed to Agricultural Pollution Using Multi-Biomarker and Multivariate Methods, Ecotoxicol., 19, pp. 1084-1094, 2010.

Bantu, N. & Vakita, V. R., Acute Toxicity of Chlorantraniliprole to Freshwater Fish Channa Punctatus (Bloch), Advances in Zoology and Botany, 1(4), pp. 78-82, 2013.

Yongqiang, L., Gao, Y., Liang, G. & Lu, Y., Chlorantraniliprole as A Candidate Pesticide Used in Combination with The Attracticides for Lepidopteran Moths, PLoS One, 12(6), pp.1-10, 2017.

Competent Authority of Organic Food, Guidelines for Certification of Organic Food Products, Indonesian Ministry of Agriculture Jakarta, 2008. (Text in Indonesia)

Chaudhry Q, Schroder, P., Werck-Reichhart, D., Grajek, W. & Marecik, R., Prospects and Limitations of Phytoremediation for the Removal of Persistent Pesticides in the Environment, Environ. Sci. & Pollut. Res., 9(4), pp. 4-17, 2002.

Tiwari, K.K, Dwivedi, S., Mishra, S., Srivastava, S., Tripathi, R.D., Singh, N.K. & Chakraborty, S., Phytoremediation Efficiency of Portulaca Tuberosa Rox and Portulaca Oleracea L. Naturally Growing in an Industrial Effluent Irrigated Area in Vadodra, Gujrat, India, Environ. Monit. Assess., 147(1-3), pp. 15-22, 2008.

Retnaningdyah, C., Arisoesilaningsih, E. & Samino, S., Use of Local Hydromacrophytes as Phytoremediation Agent in Pond to Improve Irrigation Water Quality Evaluated by Diatom Biotic Indices, Biodiversitas, 18(4), pp. 1611-1617, 2017.

Ivansyah, K. & Retnaningdyah C., Potential of Local Hydromacrophytes for Improving the Quality of NPK Fertilizer Polluted Irrigation Water Quality with Batch Culture System, J. Biotropika, 1(3), pp. 80-84, 2013. (Text in Indonesia)

Retnaningdyah, C., The Improvement of the Quality of Polluted Irrigation Water Through a Phytoremediation Process in A Hydroponic Batch Culture System, in AIP Conference Proceedings 1908 of 8th International Conference on Global Resource Conservation (ICGRC 19-20 July 2017) Salunkhe, C., Kikuchi, A. & Govinda, B. (ed.), Green Campus Movement for Global Conservation, Malang, 2017. DOI: 10.1063/1.5012703

Retnaningdyah, C. & Arisoesilaningsih, E., Using Benthic Diatom to Assess the Success of Batch Culture System Phytoremediation Process of Water Irrigation, J. Trop. Life. Science, 8(3), pp. 259-268, 2018, DOI: 10.11594/jtls.08.03.08

Krebs, C.J., Ecological Methodology, Harper and Row Publ., New York, 1989.

EPA, Pesticide Fact Sheet: Chlorantraniliprole, Office of Prevention, Pesticides and Toxic Substances (7505P), April 2008.

Barbee, G.C., McClain, W.R., Lanka, S.K. & Stout, M.J., Acute Toxicity of Chlorantraniliprole to Non-Target Crayfish (Procambarus Clarkii) Associated with Rice-Crayfish Cropping Systems, Pest. Manag. Sci, 66(9), pp. 996-1001, 2010. DOI:10.1002/ps.1972

Clesceri, L.S., Greenberg, A.E. & Eaton, A.D., Standard Methods for the Examination of Water and Waste Water, 20th Ed., Washington, 1998.

Edmondson W.T., Freshwater Biology, Second Ed., John Wiley and Sons Inc., New York, 1959.

Prescott, G.W., How to Know the Fresh Water Algae, 3rd Edition, Wm.C. Brown Company Publisher, Iowa, 1978.

Wu, N., Schmalz, B. & Fohrer, N., Study Progress in Riverine Phytoplankton and Its Use as Bio-Indicator-A Review, Austin J. Hydrol., 1(1), p. 9, 2014.

Kelly, M.G., Use of The Trophic Diatom Index to Monitor Eutrophication in Rivers, Wat. Res., 32(1), pp. 236-242, 1998.

Kelly, M.G. & Whitton, B.A., The Trophic Diatom Index: A New Index for Monitoring Eutrophication in Rivers, J. of Appl. Phyco., 7(4), pp. 433-444, 1995.

Wurts, W.A. & Durborow, R.M., Interactions of pH, Carbon Dioxide, Alkalinity and Hardness in Fish Ponds, SRAC Publication, (464), Southern Regional Aquaculture Center, December 1992.

Goldman, C.R. & Horne A.J., Limnology, Mc. Graw Hill International Book Co., New York, 1983.

Ayers R.S. & Westcot, D.W., Water Quality for Agriculture. FAO Irrigation and Drainage Paper, 29 Rev. 1, Rome, 1994.

Retnaningdyah, C. & Arisoesilaningsih, E., Ecological Significance of Irrigation Channel Riparian to Improve Benthic Macroinvertebrate Diversity, Oral presentation on International Conference on Global Resource Conservation (ICGRC), Malang, Indonesia, 7-8 February 2013.

Reynolds, C.S., The Ecology of Phytoplankton, Cambridge University Press, Cambridge, 2006.

Pasisingi, N., Pratiwi, N.M.T.M. & Krisanti, M., The Use of Trophic Diatom Index to Determine Water Quality in the Upstream of Cileungsi River, West Java, Ind. Fish. Res. J., 20(1), pp. 11-16, 2014.

Ren, Y., Wei, J., Ren, Z., Pei, H., Hu, W. & Feng, Y., A Three-Year Investigation of Planktonic Diatoms and Their Indicative Potential in Dongping Lake, China. Fresenius Env. Bulletin, 25(9), pp. 3570-3578, 2016.