Production of Ethanol from Cocoa Pod Hydrolysate
DOI:
https://doi.org/10.5614/itbj.sci.2011.43.2.2Abstract
Cocoa pod (Theobroma cacao L.) hydrolysate was hydrolyzed into glucose using hydrochloric, sulphuric and nitric acids, respectively. The concentration of each acid was set at 0.25 M, 0.50 M, 0.75 M, 1.00 M and 1.25 M. They were treated under two different temperatures and time at 75C and 90C for 2 h and 4 h, respectively. The results showed that hydrolysis in 1.00 M of hydrochloric acid at 75C for 4 h had produced the highest glucose content of 30.7% w/v compared to all others acids treated under similar conditions. The pods hydolysate was then fermented in batch culture using Saccharomyces cerevisiae for 48 h at 30C. A maximum ethanol production of 17.3%v/v was achieved after 26 h of fermentation time.References
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