Dynamics of Cocoa Bean Pulp Degradation during Cocoa Bean Fermentation: Effects of Yeast Starter Culture Addition
Keywords:cocoa bean, fermentation, pulp degradation, starter, yeast.
AbstractFermentation is a crucial step in the post-harvest processing of cocoa beans. This process comprises mixed culture microbial activities on the cocoa bean pulp, producing metabolites that act as important precursors for cocoa flavour development. Variations in the microbial population dynamics during the fermentation process may induce changes in the overall process. Thus, the introduction of a specific microbial starter culture may improve the quality of the fermentation. This article discusses the effects ofthe addition of Saccharomyces cerevisae var. Chevalieri starter culture on cocoa bean fermentation. The dynamics in the yeast concentration, sugary pulp compounds and metabolic products were measured during fermentation. The alterations in the dynamic metabolite profile were significant, although only a slight difference was observed in the yeast population. A higher fermentation index was measured for the cocoa bean fermentation with yeast starter culture, 1.13 compared to 0.84. In conclusion, this method can potentially be applied to shorten the cocoa bean fermentation time.
Schwan, R.F. & Wheals, A.E., The Microbiology of Cocoa Fermentation and its Role in Chocolate Quality, Crit Rev Food Sci Nutr., 44(4), pp. 205-21, 2004.
Thompson, S., Miller, K. & Lopez, A., Cocoa and Coffee, in Food Microbiology Fundamentals and Frontiers, M.P. Doyle, L.R. Beuchat & Montville, T.J., eds., Washington DC: ASM Press, pp. 721-736, 2001.
Ardhana, M.M. & Fleet, G.H., The Microbial Ecology of Cocoa Bean Fermentations in Indonesia, Int. J. Food Microbiol., 86(1-2), pp. 87-99, 2003.
Kustyawati, M.E. & Setyani, S., Effects of Mixed Starter Addition to the Chemicals and Microbiological Changes during Cocoa Bean Fermentation (Text in Indonesian), Jurnal Teknologi Industri dan Hasil Pertanian, 13(2), pp. 73-84, 2008.
Away, Y., Evaluation on The Effect of Some Microorganism Genera In The Fermentation Process of Cocoa Seeds to Flavor Quality and Fermentation Index (Text in Indonesian), Master Thesis, Bandung: Institut Teknologi Bandung,1989.
Atlas, R.M., Handbook of Microbiological Media, 4th ed., CRC Press, Boca Raton, 2010.
Stoll, L., Biochemische Indikatoren f1/4r Keimung und Fermentation in Samen von Kakao (Theobroma cacao L.), Universitat Hamburg, Dissertation, 2010.
Kresnowati, M.T.A.P., Ardina, A.B. & Oetomo,V.P., From Palm Oil Waste to Valuable Products: Microbial Production of Xylitol, in 19th Regional Symposium of Chemical Engineering, Bali, Indonesia, 2012.
Gourieva, K. & Tserevitinov, O., Method of Evaluating the Degree of Fermentation of Cocoa Beans, U. Patent, Editor, 1979.
Slinkard, K. & Singleton, V.L., Total Phenol Analysis: Automation and Comparison with Manual Methods, American Journal of Enology and Viticulture, 28(1), pp. 49-55, 1977.
Nielsen, D.S., Teniola, O.D., Ban-Koffi, L., Owusu, M., Andersson, T.S. & Holzapfel, W.H., The Microbiology of Ghanaian Cocoa Fermentations Analysed Using Culture-Dependent and Culture-Independent Methods, International Journal of Food Microbiology, 114(2), p. 168-186, 2007.
Lambert, S., Cocoa Fermentation: General Aspects, 2007, http://www.canacacao.org (15 April 2013).