Induction of Matrix Metalloproteinases in Chondrocytes by Interleukin IL-1β as an Osteoarthritis Model


  • Ervi Afifah Aretha Medika Utama, Biomolecular and Biomedical Research Center, Bandung 40163, Jawa Barat
  • Tjandrawati Mozef Research Center for Chemistry, Indonesian Institute of Sciences, Serpong, Banten 15314
  • Ferry Sandra Department of Biochemistry and Molecular Biology, Faculty of Dentistry, Trisakti University, Jakarta 11440
  • Seila Arumwardana Aretha Medika Utama, Biomolecular and Biomedical Research Center, Bandung 40163, Jawa Barat
  • Dwi Davidson Rihibiha Aretha Medika Utama, Biomolecular and Biomedical Research Center, Bandung 40163, Jawa Barat
  • Hayatun Nufus Aretha Medika Utama, Biomolecular and Biomedical Research Center, Bandung 40163, Jawa Barat
  • Rizal Rizal Aretha Medika Utama, Biomolecular and Biomedical Research Center, Bandung 40163, Jawa Barat
  • Annisa Amalia Aretha Medika Utama, Biomolecular and Biomedical Research Center, Bandung 40163, Jawa Barat
  • Indra Bachtiar Stem Cell and Cancer Institute, Jakarta 13210
  • Harry Murti Stem Cell and Cancer Institute, Jakarta 13210
  • Wahyu Widowati Medical Research Center, Faculty of Medicine, Maranatha Christian University, Bandung 40164, Jawa Barat



interleukin, mesenchymal stem cell, metalloproteases, MMP genes, osteoarthritis


Osteoarthritis (OA) is a chronic disease of the joints and bones due to trauma or other joint-related diseases (secondary). Synovial inflammation commonly causes disturbance in joint homeostasis, which is associated with OA. Enzymes such as aggrecanase and metalloproteinase generate cartilage damage, mediated by tumor necrosis factor (TNF-α) and interleukin (IL)-1. Pro-inflammatory cytokines, including TNF-α, IL-1β, and IL-6, are responsible for regulation of the extracellular matrix, cartilage degradation, and apoptosis of chondrocytes. This study aimed to observe the cell viability and expression level of matrix metalloproteinases (MMP-1 and MMP-3) and tissue inhibitor metalloproteinases (TIMP-1 and TIMP-2) in human chondrocyte cells (CHON-002) induced by IL-1β. CHON-002 was induced with IL-1β (0.1, 1 and 10 ng/mL) as an OA model. The viability of the cells was measured with a 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxyme-thoxyphenyl)-2-(4-sulfophenyl)-2H-tetra zolium (MTS) assay, while expression of MMP-1, MMP-3, TIMP-1, and TIMP-2, was evaluated by RT-PCR. The viability of IL-1β-induced CHON-002 (CHON-002- IL-1β) cells at day 1 and 5 showed that treatment with up to 10 ng/mL of IL-1β was not toxic. Expression of TIMP-1 and TIMP-2 in CHON-002-IL-1β was lower compared to control, while that of MMP-1 and MMP-3 was higher compared to control. These results indicate that CHON-002 treated with 10 ng/mL IL-1β has expression patterns consistent with chondrocyte damage, so the CHON-002-IL-1β system may serve as a model for MMP induction in OA.


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