Polyelectrolyte Complex (PEC) of the Alginate-Chitosan Membrane for Immobilizing Urease

Authors

  • Dhony Hermanto Department of Chemistry, Faculty of Mathematics and Fundamental Sciences, University of Mataram, Jalan Majapahit No.62, Mataram 83125, Nusa Tenggara Barat
  • Mudasir Mudasir Department of Chemistry, Faculty of Mathematics and Fundamental Sciences, University of Gadjah Mada, Jalan Sekip Utara BLS 21, Bulaksumur, Daerah Istimewa Yogyakarta 55281
  • Dwi Siswanta Department of Chemistry, Faculty of Mathematics and Fundamental Sciences, University of Gadjah Mada, Jalan Sekip Utara BLS 21, Bulaksumur, Daerah Istimewa Yogyakarta 55281,
  • Bambang Kuswandi Chemo and Biosensor Group, Faculty of Pharmacy, University of Jember, Jalan Kalimantan I No.2, Sumbersari 68121, Jember, Jawa Timur
  • Nurul Ismillayli Department of Chemistry, Faculty of Mathematics and Fundamental Sciences, University of Mataram, Jalan Majapahit No.62, Mataram 83125, Nusa Tenggara Barat

DOI:

https://doi.org/10.5614/j.math.fund.sci.2019.51.3.8

Keywords:

alginate-chitosan, immobilizing, polyelectrolyte membrane, urease

Abstract

PEC of the alginate-chitosan membrane as supporting material for immobilizing urease was produced. This study aimed to develop a supporting material for enzyme immobilization that has high stability, a fast response time and an easy and relatively inexpensive preparation procedure. An alginate-chitosan PEC membrane was produced by reacting alginate hydrosol and chitosan (1:1 in mass) at pH 5.28, followed by mixing and drying at room temperature. The FTIR spectra, XRD patterns and SEM assay confirmed that alginate-chitosan PEC was obtained. The color change of the BTB indicator proved that urease was trapped in the cavities of the alginate-chitosan PEC membrane while the immobilized urease still showed catalytic activity. Thus, the membrane of alginate-chitosan PEC has good characteristics as a matrix for urease immobilization.

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Published

2019-12-20

Issue

Vol. 51 No. 3 (2019)

Section

Articles