Polyelectrolyte Complex (PEC) of the Alginate-Chitosan Membrane for Immobilizing Urease
DOI:
https://doi.org/10.5614/j.math.fund.sci.2019.51.3.8Keywords:
alginate-chitosan, immobilizing, polyelectrolyte membrane, ureaseAbstract
PEC of the alginate-chitosan membrane as supporting material for immobilizing urease was produced. This study aimed to develop a supporting material for enzyme immobilization that has high stability, a fast response time and an easy and relatively inexpensive preparation procedure. An alginate-chitosan PEC membrane was produced by reacting alginate hydrosol and chitosan (1:1 in mass) at pH 5.28, followed by mixing and drying at room temperature. The FTIR spectra, XRD patterns and SEM assay confirmed that alginate-chitosan PEC was obtained. The color change of the BTB indicator proved that urease was trapped in the cavities of the alginate-chitosan PEC membrane while the immobilized urease still showed catalytic activity. Thus, the membrane of alginate-chitosan PEC has good characteristics as a matrix for urease immobilization.References
Gabrovska, K., Georgieva, A., Godjevargova, T. Stoilova, O. & Manolova, N., Poly(Acrylonitrile)Chitosan Composite Membranes for Urease Immobilization, J. Biotechnol., 129(4), pp. 674-680, 2007.
David, A.E., Sun, N., Yang, V.C. & Yang, A.J., Chemically Surface Modified Gel (CSMG): An Excellent Enzyme-Immobilization Matrix for Industrial Processes, J. Biotechnol., 125(6), pp. 395-407, 2006.
Yabuki, S., Polyelectrolyte Complex Membranes for Immobilizing Biomolecules, and Their Applications to Bio-Analysis, Anal. Sci., 27(7), pp. 695-702, 2011.
Mi, F., Sung, H. & Shyu, S., Drug Release from Chitosan-Alginate Complex Beads Reinforced by a Naturally Occurring Cross-Linking Agent, Carbohydr. Polym., 48, pp. 61-72, 2002.
Berger, J., Reist, M., Mayer, J.M., Felt, O., Peppas, N.A. & Gurny, R., Structure and Interactions in Covalently and Ionically Crosslinked Chitosan Hydrogels for Biomedical Applications, Eur. J. Pharm. Biopharm., 57(1), pp. 19-34, 2004.
Sankalia, M.G., Mashru, R.C., Sankalia, J.M. & Sutariya, V.B., Reversed Chitosan-Alginate Polyelectrolyte Complex for Stability Improvement of Alpha-Amylase: Optimization and Physicochemical Characterization, Eur. J. Pharm. Biopharm., 65(2), pp. 215-232, 2007.
Meng, X., Tian, F., Yang, J., He, C.N., Xing, N. & Li, F., Chitosan and Alginate Polyelectrolyte Complex Membranes and Their Properties for Wound Dressing Application, J. Mater. Sci. Mater. Med., 21(5), pp. 1751-1759, 2010.
Becheran-Maron, L. Peniche, C. & Arg1/4elles-Monal, W., Study of the Interpolyelectrolyte Reaction Between Chitosan and Alginate: Influence of Alginate Composition and Chitosan Molecular Weight, Int. J. Biol. Macromol., 34(1-2), pp. 127-133, 2004.
Kulig, D., Zimoch-Korzycka, A., Jarmoluk, A. & Marycz, K., Study on Alginate-Chitosan Complex Formed with Different Polymers Ratio, Polymers, 8(5), pp. 1-17, 2016.
Jiang, C., Wang, Z., Zhang, X., Zhu, X., Nie, J. & Ma, G., Crosslinked Polyelectrolyte Complex Fiber Membrane Based on Chitosan-Sodium Alginate By Freeze-Drying, RSC Adv., 40(78), pp. 41551-41560, 2014.
Hermanto, D., Mudasir, M., Siswanta, D. & Kuswandi, B., Synthesis of Alginate-Chitosan Polyelectrolite Complex (PEC) Membrane and Its Physical-Mechanical Properties, J. Kim. Sains dan Apl., 22(1), pp. 11-16, 2019.
Smitha, B., Sridhar, S. & Khan, A. A., Chitosan-Sodium Alginate Polyion Complexes As Fuel Cell Membranes, Eur. Polym. J., 41(8), pp. 1859-1866, 2005.
Wang, Z., Zhang, X., Gu, J., Yang, H., Nie, J. & Ma, G., Electro-deposition of Alginate/Chitosan Layer-By-Layer Composite Coatings on Titanium Substrates, Carbohydr. Polym., 103(1), pp. 38-45, 2014.
Qi, L., Xu, Z., Jiang, X., Hu, C. & Zou, X., Preparation and Antibacterial Activity oif Chitosan Nanoparticles, Carbohydr. Res., 339(16), pp. 2693-2700, 2004.
Kim, S.G., Lim, G.T., Jegal, J. & Lee, K.H., Pervaporation Separation of MTBE (Methyl Tert-Butyl Ether) and Methanol Mixtures through Polyion Complex Composite Membranes Consisting of Sodium Alginate/ Chitosan, J. Memb. Sci., 174(1), pp. 1-15, 2000.
Cheong, M. & Zhitomirsky, I., Colloids and Surfaces A"i: Physicochemical and Engineering Aspects Electrodeposition of Alginic Acid and Composite Films, 328, pp. 73-78, 2008.
Lee, J.W., Kim, S.Y., Kim, S.S., Lee, Y.M., Lee, K.H. & Kim, S.J., Synthesis and Characteristics of the Interpenetrating Polymer Network Hydrogel Composed of Chitosan and Poly(Acrylic Acid), J. Appl. Polym. Sci., 73, pp. 113-120, 1999.
Tsuchida, E., Formation of Polyelectrolyte Complexes and Their Structures, J. Macromol. Sci. Part A, 31(1), pp. 1-15, 1994.
Homaei, A.A., Sariri, R., Vianello, F. & Stevanato, R., Enzyme Immobilization"i: An Update, J. Chem. Biol., 6, pp. 185-205, 2013.
Kress, J., Zanaletti, R., Amour, A., Ladlow, M., Frey, J.G. & Bradley, M., Enzyme Accessibility and Solid Supports: Which Molecular Weight Enzymes can be Used on Solid Supports"i? An Investigation using Confocal Raman Microscopy, Chem. Eur. J., 8(16), pp. 3769-3772, 2002.