Antibacterial Curcuma xanthorrhiza Extract and Fractions


  • Hartiwi Diastuti Organic Chemistry Division, Institut Teknologi Bandung, Jalan Ganesha 10, Bandung 40132, Indonesia Chemistry Program, Universitas Jenderal Soedirman, Jl. HR Boenyamin 708 Purwokerto 53122. Indonesia
  • Yana M. Syah Organic Chemistry Division, Institut Teknologi Bandung, Jalan Ganesha 10, Bandung 40132, Indonesia
  • Lia D. Juliawaty Organic Chemistry Division, Institut Teknologi Bandung, Jalan Ganesha 10, Bandung 40132, Indonesia
  • Marlia Singgih School of Pharmacy, Institut Teknologi Bandung, Jalan Ganesha 10, Bandung, Indonesia



α-curcumene, antibacterial, Curcuma xanthorrhiza, curcumin, xanthorrhizol, NMR analysis, TOCSY-1D


An acetone extract of Curcuma xanthorrhiza rhizomes and the n-hexaneand chloroform fractions obtained from it were tested on eightpathogenic bacteria. The results showed that the acetone extract and the nhexanefraction exhibited significant activities against Bacillus subtilis,Pseudomonas aeruginosa, and Staphylococcus aureus, and weak activitiesagainst Shigella dysenteriae and Vibrio cholerae. They were inactive againstEscherichia coli, Enterobacter aerogenes and Salmonella thypi, while thechloroform fraction was devoid of activities. NMR analysis disclosed thepresence of α-curcumene, xanthorrhizol and an unknown monoterpene in the nhexanefraction. In the chloroform fraction, curcumin was found to be the maincompound, together with xanthorrhizol as a minor compound. These resultssuggest that the antibacterial potency of acetone extract of C. xanthorrhiza iscontained in the n-hexane fraction, in which the active constituents are terpenoidcompounds. This is the first report of the use of NMR analysis for compoundidentification contained in an extract or fractions of C. xanthorrhiza.


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