Ultrastructure of Septa in Sclerotinia sclerotiorum
Abstract
Abstract. An electron microscope study has been done on the structure of septum and its associations ofSclerotiniasclerotiorum(Lib.) de Bary. This septum consists of a cross-wall containing two electron-dense layers separated by an electron-transparent central lamella. The central pore in the cross-wall is partially to completely blocked by cell inclusions called Woronin bodies, which are electron-dense and membrane-bound.
Ringkasan. Pengamatan jamur penyebab penyakit tumbuhan, Sclerotiniasclerotiorum(Lib.) de Bary dengan menggunakan mikroskop elektron telah dikerjakan dalam laporan ini, terutama sekali mengenai dinding melintang dari pada hypha dan benda-benda sel lainnya yang selalu berhubungan erat sekali dengan celah di antara dinding melintang, yang disebut "Woronin bodies". Dinding melintang, tediri dari tiga lapis, yaitu dua lapis yang banyak menerima electron (electron-dense layers) dan dibagian tengahnya dipisahkan oleh selapis bagian yang tembus cahaya (electron-transparent central lamella). Celah pada dinding melintang sering sekali disumbat oleh "Waronin bodies".
References
Bracker, C.E. 1967. Ultrastructure of fungi. Ann. Rev. Phytopathol., 5 : 343-374.
Bracker, C.E. and E.E. Butler, 1963. The Ultrastructure and development of septa in hyphae of Rhizoctonia solani. Mycologia, 55 : 35-58.
Brenner, D.M. and G.C. Carroll. 1968. Fungi-structural Correlates of growth in hyphae of Ascodesmis sphaerospora. J. Bacteriol., 95 : 658-671.
Buller, A.H.R. 1958. Researches of fungi. Vol. V. Hafner Publishing Co., New York.
Calonge, F.D. 1970. Notes on the ultrastructure of the Microconidium and stroma in Sclerotinia sclerotiorum. Arch. Mikrobiol, 71 : 191-195.
Calonge, F.D. Fielding, A.H. and R.J.W. Byrde. 1969. Multivesicular bodies in Sclerotinia fructigena and their possible relation to extracellular enzyme secretion. J. Gen. Microbiol., 55 : 177-184.
Carbonell, L.M. and J. Rodriguez. 1968. Mycelial phase of Paracoccidioides brasiliensis and Blastomyces dermatitidis: an electron microscopis study. J. Bacteriol., 96 : 533-543.
Furtado, J.S. 1971. The septal pore and other Ultrastructural features of the Pyrenomycete Sordaria fimicola. Mycologia, 63 : 104-113.
Hess, W.M. 1966. Fixation and staining of fungus hyphae and host plant root tissues for electron microscopy. Stain Technology, 41 : 27-35.
Jones, D. 1970. Ultrastructure and composition of the cell walls of Sclerotinia sclerotiorum. Trans. BR. mycol. Soc. 54 (3) : 351-360.
Juniper, B.E., Gilchrist, A.J., Cox, G.C. and P.R. Williams, 1970. Techniques for plant electron microscopy. Blackwell scientific Publications. Oxford and Edinburgh.
Littlefield, I.J. and C.E. Bracker, 1971. Ultrastructure of septa in Melampsore lini. Trans. Br. mycol. Soc. 56 (2) : 181-188.
Mercer, E.H. and M.S.C. Birbeck. 1961. Electron Microscopy. A Handbook for Biologists. Blackwell Scientific Publications. Oxford.
Moore, R.T. and J.H. McAlear. 1962. Fine structure of mycota. 7. Observations of septa of Ascomycetes and Basydiomycetes. Am. J. Botany, 49 : 86-100.
Reichle, R.E. and J.V. Alexander. 1965. Multiperforate septations, Woronin bodies and septal plugs in Fusarium. J. Cell Biol., 24 : 489-496.
Reynolds, E.S. 1963. The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J. Biophys. Biochem. Cytol. 17 : 208.
Sabatini, D.D., Bensch, K. and R.J. Barnett. 1963. Cytochemistry and electron microscopy. J. Cell Biol. 17 : 19-58.
Shatkin, A.J. and E.L. Tatum. 1959. Electron microscopy of Neurospora crassa. mycelia. J. Biochem. Biophys. Cytol., 6 : 423-426.
Willetts, H.J. and F.D. Calonge. 1969. The Ultrastructure of the Stroma of the Brown Rot Fungi. Arch. Mikrobiol, 64 : 279-288.
