ANTIOXIDANT CAPACITY OF THE ETHYL ACETATE FRACTION OF JATROPHA CURCAS L. LEAVES USING THE FERRIC REDUCING ANTIOXIDANT POWER (FRAP) METHOD
DOI:
https://doi.org/10.5614/api.v51i1.25186Keywords:
Jatropha curcas leaves, Jatropha curcas, Antioxidant, FRAP methodAbstract
Jatropha curcas L. leaves are known to be rich in bioactive compounds with strong antioxidant activity. This study aimed to evaluate the antioxidant capacity of the ethyl acetate fraction of Jatropha curcas leaf extract using the Trolox Equivalent Antioxidant Capacity (TEAC) method. The process began with extraction using 96% ethanol, followed by fractionation with ethyl acetate. Antioxidant activity was measured using a UV-Vis spectrophotometer, and the antioxidant concentration was determined using the Trolox standard curve. The results showed that the ethyl acetate fraction exhibited exceptionally high antioxidant capacity, with an average value of 9.192 0.380 ?mol TE/g. This value far exceeded the highest category in Bartolini?s classification. Phenolic compounds were identified as the primary contributors to this antioxidant activity, while flavonoids also contributed, albeit to a lesser extent. Differences in antioxidant capacity compared to other studies may be attributed to variations in extraction methods, solvent types, and raw material conditions. This underscores the importance of selecting appropriate extraction methods and solvents to maximize the antioxidant potential of natural products. With its significant antioxidant capacity, the ethyl acetate fraction of Jatropha curcas leaves holds great potential for further development in the pharmaceutical and health sectors as a natural source of antioxidants.
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