ANTIOXIDANT ACTIVITY OF ROSELA FLOWER EXTRACT LOTION (HIBISCUS SABDARIFA L.) WITH VARIOUS CONCENTRATIONS OF HONEY AS HUMECTANT
DOI:
https://doi.org/10.5614/api.v51i1.26468Keywords:
Antioxidant, Rosella Flower, Humectant, Lotion, HoneyAbstract
Roselle plants (Hibiscus sabdariffa L.) contain several phytochemical compounds including phenols, alkaloids, tannins, flavonoids, saponins and anthocyanins. One of the compounds found in rosella flowers, such as anthocyanin, which is a type of flavonoid, has benefits as an antioxidant. This study aims to determine the effect of variations in the concentration of calliandra honey, used as a humectant, on the physical characteristics and antioxidant activity of Rosela Flower Extract Lotion preparations. The physical characteristics of the preparation include organoleptic test, homogeneity, viscosity, spreadability, pH, stability test, and antioxidant activity test. The antioxidant activity test used the 2,2-diphenyl-1-picryhydrazyl (DPPH) method and then calculated the percent inhibition. Three formulas of lotion were made with a 2.5% concentration of roselle flower extract and a variation in the concentration of calliandra honey (1.5%; 2%; 2.5%). The positive control was 2% ethyl vitamin C lotion. The study showed that the lotion of roselle flower extract with calliandra honey concentration met the SNI standard. The concentration variation of calliandra honey can increase its antioxidant activity, where in F1 with IC50 value of 111.0 ?g/mL; F2 108.29 ?g/mL; F3 95.20 g/mL; F4 an IC50 value of 106.29 ?g/mL; F5 111.71 ?g/mL; and F6 108,13 ?g/mL. Data were statistically analyzed using the Kolmogorov-Smirnov test followed by one-way ANOVA with 95% confidence level. From this study, it can be concluded that the lotion formula with an extract concentration of 2.5% combined with 2.5% honey has an IC50 value of 106.29 ?g/mL.
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