Pengembangan Perangkat Surface Plasmon Resonance (SPR) sebagai Transduser Biosensor

Fadli  Hastito; Endang  Juliastuti; Brian  Yuliarto

doi:10.5614/joki.2025.17.2.3

Authors

Fadli Hastito Institut Teknologi Sumatera, 35365, Lampung, Indonesia
Endang Juliastuti Institut Teknologi Bandung, 40132, Bandung, Indonesia
Brian Yuliarto Institut Teknologi Bandung, 40132, Bandung, Indonesia

Keywords:

surface plasmon resonance (SPR), biosensor, kretschmann configuration, glucose detection, portable optical device

Abstract

This study developed a simple and cost-effective laboratory-scale Surface Plasmon Resonance (SPR) device called β SPR. SPR is a sensitive, real-time, and non-labeling technique widely used to detect the concentration and quality of solutions. However, the very high price of commercial SPR devices is a barrier, so a portable and affordable version was developed. The β SPR device uses a Kretschmann configuration with a 670 nm laser, a polarizer, and a modified Porro BA4010 prism for a simpler and more efficient optical configuration. A thin gold film (~50 nm) is placed on the prism using immersion oil, and the test solution is flowed through a flow cell. The laser is fired at a 90° angle to induce p-polarized waves that trigger surface plasmon resonance. This phenomenon decreases the light reflectance, forming a dip curve used for analysis. The device was tested using glucose solution (0.05–0.27 M) and compared with a commercial SPR device (α SPR). The results show a shift in the angle with increasing concentration. The highest error was 6.53% at 0.05 M, and the lowest was 0.94% at 0.27 M. The β SPR sensitivity was recorded at 4.41⁰/M, showing promising performance for cost-effective biosensor applications.

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